Article
Oncology
Jacob Bickels, Yair Gortzak, Amir Sternheim
Summary: The use of 5-ALA photodynamic therapy can decrease the likelihood of local tumor recurrence in fibroblastic soft-tissue tumors.
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2023)
Article
Oncology
Abel Ferres, Alberto Di Somma, Alejandra Mosteiro, Thomaz Eduardo Topczewski, Pedro Roldan, Leire Pedrosa, Dioulde Diao, Estela Pineda, Angels Sierra, Joaquim Ensenat, Jose Juan Gonzalez-Sanchez
Summary: In this study, an inadvertent effect of 5-ALA-mediated photodynamic therapy has been detected in vivo. This finding opens up possibilities for further research on this promising therapeutic tool.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Elizaveta Kozlikina, Igor S. Trifonov, Mikhail Sinkin, Vladimir V. Krylov, Victor B. Loschenov
Summary: The treatment of glial brain tumors is a challenging problem in neuro-oncology. This case report demonstrates the potential of using a combination of two photosensitizers for fluorescence-guided resection and photodynamic therapy to achieve total tumor removal and additional treatment.
BIOENGINEERING-BASEL
(2022)
Review
Pharmacology & Pharmacy
Liang Lou, Shizhe Zhou, Sijia Tan, Menghua Xiang, Wei Wang, Chuang Yuan, Liqian Gao, Qicai Xiao
Summary: ALA is a clinically approved prodrug that can be used to synthesize the natural photosensitizer PpIX. However, its weak lipophilicity, low stability, and poor bioavailability limit its clinical performance. Nanotechnology offers a solution to improve the therapeutic outcomes of ALA-based prodrugs.
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Pharmacology & Pharmacy
Chunpeng Zhang, Xuewen Zhao, Danlu Li, Fengqi Ji, Andi Dong, Xi Chen, JiaHui Zhang, Xiaoxiong Wang, Yilei Zhao, Xin Chen
Summary: Photodynamic therapy (PDT) is a novel therapy that uses specific wavelengths of light to activate photosensitizers and treat tumors. 5-aminolevulinic acid (5-ALA) is a commonly used photosensitizer, but its permeability and targeting are limited. Researchers have developed various 5-ALA nano-delivery systems and achieved good efficacy in tumor treatment.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hung Wei Lai, Kiwamu Takahashi, Motowo Nakajima, Tohru Tanaka, Shun-ichiro Ogura
Summary: The study demonstrated that ALA-PDT is more effective in cancer cells of higher malignancy, with increased PpIX production, upregulation of ALA uptake transporters and YAP expression in correlation with higher cancer cell density.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Optics
Anastasia Ryabova, Igor Romanishkin, Alexey Skobeltsin, Inessa Markova, Daria Pominova, Kirill Linkov, Victor Loschenov
Summary: By analyzing the fluorescence lifetime of macrophages, the polarization state of macrophages in tumors can be detected non-invasively, which is important for post-surgery tissue inspection.
Article
Oncology
Stefanie Lietke, Michael Schmutzer, Christoph Schwartz, Jonathan Weller, Sebastian Siller, Maximilian Aumiller, Christian Heckl, Robert Forbrig, Maximilian Niyazi, Rupert Egensperger, Herbert Stepp, Ronald Sroka, Jorg-Christian Tonn, Adrian Ruhm, Niklas Thon
Summary: Malignant glioma, especially in recurrent situations, has a poor prognosis. Interstitial photodynamic therapy (iPDT) using 5-aminolevulinic acid-induced protoporphyrin IX as a cytotoxic photosensitizer shows promising results in treating recurrent malignant gliomas. Heterogeneous treatment response was observed without significant association with patient characteristics, treatment-related factors, or molecular markers. Further prospective evaluation is needed to identify mechanisms and prognostic factors of favorable treatment response.
Article
Biochemistry & Molecular Biology
Marlene Aparecida Ferreira Pinto, Cassia Bosi Ribeiro Ferreira, Barbara Evelyn Santos de Lima, Angela Cristina Molon, Ana Melissa Coppa Ibarra, Rebeca Boltes Cecatto, Adriana Lino dos Santos Franco, Maria Fernanda Setlibal Destro Rodrigues
Summary: The present study found that PDT using 5-aminoulevulinic acid as a photosensitizer can reduce the proportion of cancer stem cells and decrease their functional capabilities, inducing differentiation.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Airong Li, Chenglin Liang, Lihua Xu, Yiyang Wang, Wei Liu, Kaixiang Zhang, Junjie Liu, Jinjin Shi
Summary: A liposomal nanomedicine with iron ion regulation property has been developed to enhance the photodynamic therapy of 5-ALA. By co-encapsulating 5-ALA and DFO into membrane fusion liposomes, the drug delivery efficiency to tumor cells was improved, leading to the accumulation of photosensitive substances and aggravated DNA damage through iron ion inhibition.
ACTA PHARMACEUTICA SINICA B
(2021)
Article
Oncology
Nian Chen, Xia Lei, Qionghui Cheng
Summary: This article reports a case of intractable Queyrat erythroplasia involving the urethral meatus. The study suggests that 5-aminolaevulinic acid photodynamic therapy is effective and safe in the treatment of Queyrat erythroplasia, providing a new choice for patients with poor therapeutic effect.
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2023)
Article
Dermatology
Jon Chim Bai-Habelski, Annette Ko, Christoph Ortland, Marcus Stocker, Andrea Ebeling, Uwe Reinhold
Summary: Photodynamic therapy with 5-aminolevulinic acid hydrochloride is an established method for treating actinic keratosis (AK). Occlusion during ALA incubation can improve the clinical outcome of PDT for AK on hands and arms. Two PD P 506 A-PDT sessions 1-2 weeks apart are an efficacious treatment for AK on hands and arms.
EXPERIMENTAL DERMATOLOGY
(2022)
Article
Oncology
Hang Shi, Jiyang Li, Cheng Peng, Beiyun Xu, Hongying Sun
Summary: The study demonstrated that PDT using 5-aminolevulinic acid as a photosensitizer had significant inhibitory activity against C. albicans biofilms. It induced cell apoptosis, cytoplasm degradation, nuclear condensation, and mitochondrial swelling, and reduced mRNA expressions of specific genes. This suggests that ALA-PDT may serve as a potential antifungal strategy for eliminating C. albicans biofilms.
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2021)
Article
Chemistry, Physical
Fei Huang, Guiyan Han, Xingjie Zhang, Yu Li, Zhenyuan Miao, Jianzhong Yao
Summary: In this study, a novel chlorin-based photosensitizer was designed and synthesized for tumor photodynamic therapy. The photosensitizer showed high phototoxicity, high dark toxicity/phototoxicity ratio, and excellent PDT antitumor efficacy in vitro and in vivo.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Review
Chemistry, Medicinal
Yo Shinoda, Daitetsu Kato, Ryosuke Ando, Hikaru Endo, Tsutomu Takahashi, Yayoi Tsuneoka, Yasuyuki Fujiwara
Summary: The study reviewed and analyzed the effectiveness of 5-ALA PDT in vitro experiments for different cancer cell types, finding that the sensitivity to 5-ALA PDT varies across cancer classifications, with stomach cancer showing significantly higher sensitivity compared to other cancers. The analysis suggests the need for a standardized in vitro experimental protocol for 5-ALA PDT in future research.
Editorial Material
Biochemistry & Molecular Biology
Yasutoshi Koga, Yau-Huei Wei, Masashi Tanaka
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2020)
Article
Biochemistry & Molecular Biology
Hsin-Chung Lin, Yu-Jen Chen, Yau-Huei Wei, Yu-Ting Chuang, Su-Heng Hsieh, Jing-Yu Hsieh, Yi-Lin Hsieh, David M. Ojcius, Kuo-Yang Huang, I-Che Chung, Sheng-Ning Yuan, Yu-Sun Chang, Lih-Chyang Chen
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Review
Biochemistry & Molecular Biology
Chih-Hao Wang, Yau-Huei Wei
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Immunology
Hsin-Chung Lin, Yu-Jen Chen, Yau-Huei Wei, Hsin-An Lin, Chien-Chou Chen, Tze-Fan Liu, Yi-Lin Hsieh, Kuo-Yang Huang, Kuan-Hung Lin, Hsueh-Hsiao Wang, Lih-Chyang Chen
Summary: The study found that lactic acid fermentation is essential for NLRP3 inflammasome activation, while pyruvate oxidation is dispensable. Inhibiting lactic acid fermentation can reduce inflammasome activation, while inhibiting pyruvate oxidation enhances inflammasome activation.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Chih-Hao Wang, Yau-Huei Wei
Summary: Obesity and related metabolic disorders like type 2 diabetes are becoming increasingly prevalent worldwide, prompting the exploration of strategies to combat these conditions by activating brown adipose tissue and converting white adipose tissue to beige fat to increase energy expenditure. Recent advancements in utilizing thermogenic adipocytes through genetic, pharmacological, and cell-based approaches for the treatment of obesity and metabolic disorders in mice and humans are promising for therapeutic potential.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Wei-Kuang Yu, Wei-Lun Hwang, Yi-Chuan Wang, Chieh-Chih Tsai, Yau-Huei Wei
Summary: Curcumin inhibits TGF-beta 1-induced myofibroblast differentiation and attenuates the pro-angiogenic activity of orbital fibroblasts. This suggests the potential application of curcumin for the treatment of Graves' ophthalmopathy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Yao-An Shen, Chang-Cyuan Chen, Bo-Jung Chen, Yu-Ting Wu, Jiun-Ru Juan, Liang-Yun Chen, Yueh-Chun Teng, Yau-Huei Wei
Summary: Cancer stem cells (CSCs) play a crucial role in cancer treatment and progression, with unique metabolic characteristics that differ from normal cells. Studying the metabolic pathways that CSCs rely on can provide new opportunities and methods for cancer treatment.
Article
Biochemistry & Molecular Biology
Tzu-Ching Chang, Hui-Ting Lee, Siao-Cian Pan, Shih-Han Cho, Chieh Cheng, Liang-Hung Ou, Chia- Lin, Chen-Sung Lin, Yau-Huei Wei
Summary: We investigated the roles of mitochondrial DNA (mtDNA) alterations and mitochondrial respiratory dysfunction in gastric adenocarcinoma (GAC) using gastric cancer cell line AGS and clinical samples. The study found that decreased mtDNA copy number and D310 mutation were associated with the development and prognosis of GAC, and mitochondrial dysfunction affected the biological characteristics of GAC cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Ching-Mao Chang, Po-Chang Wu, Jr-Rung Lin, Yeong-Jian Jan Wu, Shue-Fen Luo, Yin-Tzu Hsue, Joung-Liang Lan, Tai-Long Pan, Yu-Ting Wu, Kuang-Hui Yu, Yau-Huei Wei, Hen-Hong Chang
Summary: The study evaluated the therapeutic efficacy of herbal formula SS-1 for patients with Sjogren's syndrome. SS-1 exhibited anti-proliferative activity in B cells and promoted anti-inflammatory effects. Clinical trials demonstrated significant improvements in symptoms and reduction in artificial tear use, suggesting SS-1 as a potential complementary treatment for Sjogren's syndrome.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Cell Biology
Chih-Hao Wang, Chen-Hung Wang, Pen-Jung Hung, Yau-Huei Wei
Summary: The prevalence and healthcare burden of obesity and its related metabolic disorders such as type 2 diabetes (T2D) are increasing rapidly. Mitochondria and endoplasmic reticulum (ER) play important roles in insulin resistance and T2D, and their dysfunction may contribute to the progression of these diseases. In this study, the authors found that the formation of mitochondria-associated ER membranes (MAMs) is increased during the differentiation of preadipocytes, and disruption of MAMs impairs cell differentiation and insulin signaling. Additionally, knocking down the protein GRP75, which is involved in MAMs formation, also impairs thermogenesis and glucose utilization in brown adipocytes. These findings suggest that MAMs formation is essential for the proper functioning of adipocytes and insulin signaling, and may be a potential target for therapeutic interventions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Yu-Ting Wu, Hui-Yi Tay, Jung-Tse Yang, Hsiao-Hui Liao, Yi-Shing Ma, Yau-Huei Wei
Summary: In this study, patient-derived iPSCs were used to model neurons and investigate the pathogenic mechanism of neurological disorders in MERRF syndrome. The results showed that MERRF neural cells harboring the m.8344A > G mutation exhibited impaired mitochondrial bioenergetic function, elevated ROS levels and imbalanced expression of antioxidant enzymes. Electrophysiological recordings also revealed impairment of neuronal activities in neurons with a high level of the m.8344A > G mutation.
JOURNAL OF BIOMEDICAL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Jagdeep K. Sandhu, Caroline Sodja, Maria Ribecco-Lutkiewicz, Yu-Ting Wu, Yi-Shing Ma, Yau-Huei Wei, Marianna Sikorska
Summary: Mitochondrial biogenesis occurs during neuronal differentiation, leading to improved cellular energy status and enhanced tolerance to oxidative stress, casting doubt on the potential for additional enhancement of this phenomenon for neuroprotection.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2022)
Review
Urology & Nephrology
Tzu-Yu Chuang, Te-Wei Chang, Shiou-Sheng Chen, Chan-Chi Chang, Wei-Ming Cheng, Yau-Huei Wei
Summary: Mitochondria are essential organelles responsible for energy production and regulating various cellular processes. The prevalence of primary mitochondrial disease is estimated to be around 1:5000, with implications in bladder dysfunction, reproductive disorders, and bladder cancer due to mtDNA mutations.
UROLOGICAL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Haifeng Jiao, Dong Jiang, Xiaoyu Hu, Wanqing Du, Liangliang Ji, Yuzhuo Yang, Xiaopeng Li, Takami Sho, Xuan Wang, Ying Li, Yu-Ting Wu, Yau-Huei Wei, Li Yu, Xiaoyu Hu
Summary: Mitocytosis is a migrasome-mediated mitochondrial quality-control process that helps maintain mitochondrial quality in migrating cells. The process involves positioning damaged mitochondria at the cell periphery, which avoids binding to inward motor proteins. Enhanced mitocytosis protects cells from mitochondrial stressor-induced loss of mitochondrial membrane potential and mitochondrial respiration, while blocking mitocytosis causes loss of these functions under normal conditions. Physiologically, mitocytosis is required for maintaining mitochondrial membrane potential and viability in neutrophils in vivo.
Article
Surgery
Chen-Sung Lin, Yi-Chen Yeh, Siao-Cian Pan, Shih-Yu Lu, Yann-Jang Chen, Wen-Yu Chueh, Yau-Huei Wei
FORMOSAN JOURNAL OF SURGERY
(2020)
