Journal
OPTICS EXPRESS
Volume 19, Issue 12, Pages 11732-11739Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.19.011732
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- National Science Council (NSC) of Taiwan [NSC 97-3112-B-006-013, NSC 97-3111-B-006-004]
- Advanced Optoelectronic Technology Center in National Cheng Kung University
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In this study, the intensity of two-photon excited fluorescence (TPEF) of xanthene dye, Rose Bengal (RB), was significantly enhanced via bovine serum albumin (BSA) microstructures fabricated by the two-photon crosslinking (TPC) technique. The RB was utilized as the photoactivator in the TPC processing and the enhanced TPEF intensity correlates with the concentration of fabricated crosslinked BSA microstructures via the power control and pulse selection of the employed femtosecond laser. As a result, fabrication of three-dimensional BSA microstructures can be simultaneously monitored by the use of TPEF intensity. The crosslinked BSA microstructures synthesized may be used as an ordered biomaterial for fluorescence enhancement. (C) 2011 Optical Society of America
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