Journal
OPTICS EXPRESS
Volume 18, Issue 19, Pages 20049-20058Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.18.020049
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Funding
- NSF [DBI 0852891]
- Center for Biophotonics Science and Technology, NSF Science and Technology Center, University of California, Davis [PHY0120999]
- Direct For Biological Sciences
- Div Of Biological Infrastructure [0852891] Funding Source: National Science Foundation
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A time gating system has been constructed that is capable of recording high quality Raman spectra of highly fluorescing biological samples while operating below the photodamage threshold. Using a collinear gating geometry and careful attention to power conservation, we have achieved all-optical switching with a one picosecond gating time and 5% peak gating efficiency. The energy per pulse in this instrument is more than 3 orders of magnitude weaker than previous reports. Using this system we have performed proof-of-concept experiments on a sample composed of perylene dissolved in toluene, and the stem of a Jasminum multiflorum plant, the latter case being particularly important for the study of plants used in production of cellulosic biofuels. In both cases, a high SNR spectrum of the high-wavenumber region of the spectrum was recorded in the presence of an overwhelming fluorescence background. (C) 2010 Optical Society of America
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