Article
Computer Science, Interdisciplinary Applications
Mohammadreza Amjadian, Seyed Masood Mostafavi, Jiangbo Chen, Zahra Kavehvash, Jingyi Zhu, Lidai Wang
Summary: This paper proposes a novel super-resolution volumetric photoacoustic microscopy based on structured illumination theory, utilizing optical excitation and Fourier-domain reconstruction algorithm to improve lateral resolution and reduce computational load. Experimental results demonstrate significant enhancements in lateral resolution and signal-to-noise ratio of the imaging system.
IEEE TRANSACTIONS ON MEDICAL IMAGING
(2021)
Article
Optics
Jiazhen Dou, Chen Dong, Siqing Dai, Jingyu Mi, Xiangyuan Luo, Jianglei Di, Jiwei Zhang, Jianlin Zhao
Summary: This research proposes a method to enhance the spatial resolution of SPRHM by exciting surface plasmon resonance in two symmetrical directions, and successfully establishes reconstruction schemes for high-resolution amplitude and phase-contrast SPR images.
OPTICS AND LASERS IN ENGINEERING
(2022)
Article
Optics
Yao Fan, Jiasong Sun, Yefeng Shu, Zuxin Zhang, Guoan Zheng, Wenjian Chen, Jin Zhang, Kun Gui, Kehui Wang, Qian Chen, Chao Zuo
Summary: Fourier ptychographic microscopy (FPM) is a computational high-throughput technique that achieves high-resolution and wide field-of-view (FOV) imaging. This paper proposes an efficient synthetic aperture scheme for FPM, called ESA-FPM, which utilizes both coherent and incoherent illuminations to maximize data utilization and achieve high-resolution reconstruction with few acquisitions. The experiment demonstrates that ESA-FPM achieves theoretical resolution with only 1.6% of the data of conventional FPM.
LASER & PHOTONICS REVIEWS
(2023)
Article
Optics
Nisan Siegel, Gary Brooker
Summary: This new super-resolution microscopy technique utilizes a simple and versatile approach to achieve better resolution than other complex methods after a single image capture. It can work with any objective power and numerical aperture, is easy to operate and maintain, and can create super-resolved images using any type or wavelength of light.
Article
Multidisciplinary Sciences
Jeonghun Oh, Herve Hugonnet, YongKeun Park
Summary: The authors propose a holographic image sensor that can reconstruct the complex amplitude of scattered light from a single-intensity image using reciprocal diffractive imaging. This allows for holographic imaging of three-dimensional diffusive objects and potential applications in both static and dynamic conditions.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Michael J. Wester, David J. Schodt, Hanieh Mazloom-Farsibaf, Mohamadreza Fazel, Sandeep Pallikkuth, Keith A. Lidke
Summary: In this study, a robust drift correction method is proposed for super-resolution methods based on single molecule localization, which combines 3D registration with a fast post-processing algorithm, ensuring stability and drift correction for an indefinite time period without specialized hardware.
SCIENTIFIC REPORTS
(2021)
Article
Environmental Sciences
Nertjana Ustalli, Michelangelo Villano
Summary: This paper proposes two high-resolution, wide-swath SAR acquisition modes for ship monitoring that tolerate ambiguities and do not require digital beamforming. Examples show that these modes can achieve outstanding detection performance for small ships and can be widely used in open sea surveillance.
Article
Environmental Sciences
Xiaofeng Wang, Yaduan Ruan, Xinggan Zhang
Summary: This paper proposes a high-resolution wide-swath (HRWS) imaging algorithm for a spaceborne SAR with a low PRF based on optimal spectrum shift processing, in order to solve the problem of azimuth spectrum aliasing caused by low PRF. Each sub-aperture is imaged using the typical range migration algorithm (RMA), and then all sub-images are up-sampled at the same time. Based on the criterion of the minimum grating lobe, the optimal spectrum shift is estimated. Finally, the shifted sub-images are synthesized coherently. Simulation results confirm the effectiveness of the proposed method.
Article
Optics
Matthew A. Goodman, R. Krishna Mohan, Zeb W. Barber, Wm Randall Babbitt
Summary: An off-axis digital holographic imaging polarimeter was developed to estimate the Jones matrices of an object, compensating for phase variations in the optical beam paths. A minimization algorithm was used to compute an estimation of the Jones matrix image of an object based on a set of measured Jones vector images. A proof-of-concept demonstration was performed to compute an estimated Jones matrix image of a polarimetrically complex object using digital holograms recorded with 6 different illumination polarizations.
Article
Optics
Ping Su, Da Sun, Jianshe Ma, Zhenpeng Luo, Hua Zhang, Shilun Feng, Liangcai Cao
Summary: Digital holographic microscopy with compressive sensing has achieved tomography successfully, but the enhancement of axial resolution remains unclear. This study quantified the elongation effect of DH systems without paraxial approximation, revealing it as an inherent property influenced by numerical aperture. By eliminating the elongation effect with CS, the tomography of fibers was achieved and crossed fibers were successfully distinguished at different distances. There are potential applications of this theory in tomography and observation of microscale objects in biological and fluid fields.
Article
Biochemical Research Methods
David J. Schodt, Farzin Farzam, Sheng Liu, Keith A. Lidke
Summary: We introduce the Sequential Super-resolution Microscope (SeqSRM), a dedicated microscope for automated sequential localization microscopy. SeqSRM automates precise stage stabilization and data acquisition of user-selected cells, minimizing user interaction. We demonstrate that nanometer-scale changes in cell morphology can impact the fidelity of multi-target super-resolution reconstructions, but these shifts can be reliably detected and masked out using brightfield images. SeqSRM enables automated multi-target imaging without the need for multiple fluorophores, while accurately reflecting the relative organization of the targets.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Optics
Nimit Patel, Siddharth Rawat, Mugdha Joglekar, Vani Chhaniwal, Satish Kumar Dubey, Timothy O'Connor, Bahram Javidi, Arun Anand
Summary: The compact and low-cost system presented in this study is capable of Digital Holographic Microscopic imaging of immobilized micro-particles, with high accessibility and wide application prospects. Integration of DHM into the system through the use of optical pickup unit and glass plate enables particle immobilization and phase imaging, providing rich information content for in-depth analysis of immobilized micro-particles.
OPTICS AND LASERS IN ENGINEERING
(2021)
Article
Optics
Xiaoli Ji, Xiaoliang He, Zhilong Jiang, Yan Kong, Shouyu Wang, Cheng Liu
Summary: By deducing a formula and conducting analytical analysis, the relationship among the highest achievable resolution of ptychographic iterative engine (PIE), its illumination angle, and its collection angle was discussed. Curved illumination was proposed to enhance the resolution for PIE, and a corresponding computing algorithm was proposed to avoid undersampling effect without increasing the size of the computing matrix, thus enabling high-resolution PIE imaging with a simple experimental setup. The feasibility of this proposed method was verified both numerically and experimentally.
Article
Computer Science, Interdisciplinary Applications
Mohammadreza Amjadian, Seyed Masood Mostafavi, Jiangbo Chen, Lidai Wang, Zhengtang Luo
Summary: Acoustic-resolution photoacoustic microscopy (AR-PAM) system can provide 3-D images of facial tissues. The lateral resolution of AR-PAM depends on the numerical aperture (NA) of the acoustic lens and the central frequency of the ultrasonic transducer. Structured-illumination and a modified phase-shifting method can improve the lateral resolution by overcoming the diffraction limit.
IEEE TRANSACTIONS ON MEDICAL IMAGING
(2022)
Article
Optics
Luis E. Villegas-Hernandez, Vishesh Dubey, Mona Nystad, Jean-Claude Tinguely, David A. Coucheron, Firehun T. Dullo, Anish Priyadarshi, Sebastian Acuna, Azeem Ahmad, Jose M. Mateos, Gery Barmettler, Urs Ziegler, Asa Birna Birgisdottir, Aud-Malin Karlsson Hovd, Kristin Andreassen Fenton, Ganesh Acharya, Krishna Agarwal, Balpreet Singh Ahluwalia
Summary: This study introduces a photonic chip as a high-throughput microscopy platform for super-resolution imaging of histological samples. It demonstrates diverse imaging capabilities and paves the way for the adoption of super-resolution high-throughput multimodal analysis of cryopreserved tissue samples.
LIGHT-SCIENCE & APPLICATIONS
(2022)
Article
Engineering, Electrical & Electronic
Rajib Dey, Yi Zhou, Kai Neuhaus, Sergey Alexandrov, Andrew Nolan, Ting-Chiao Chang, Martin Leahy
Summary: Optical Coherence Tomography (OCT) is a widely used imaging technique in biomedical field for high-resolution imaging of biological tissues. This paper presents a fiber-based OCT system with a center wavelength of 1300 nm, achieving an axial resolution of 3.8 μm, surpassing any previously reported values. The system investigation was motivated by the increasing demand for commercialization of high-resolution OCT systems and simplification of construction. The paper also evaluates and demonstrates the direct measurement method for axial resolution using an air wedge.
IEEE PHOTONICS JOURNAL
(2022)
Article
Instruments & Instrumentation
Rachel Lloyd, Jane Walter, Sarah Pirikahu, Gemma Cadby, Martha Hickey, David D. Sampson, Karol Karnowski, Michael J. Hackmann, Christobel Saunders, Lothar Lilge, Jennifer Stone
Summary: Mammographic breast density is a significant risk factor for breast cancer, and optical breast spectroscopy (OBS) is a promising method for measuring breast density. This study validates the participant data of OBS and confirms its effectiveness in epidemiological research.
REVIEW OF SCIENTIFIC INSTRUMENTS
(2022)
Review
Optics
Danuta M. Sampson, Adam M. Dubis, Fred K. Chen, Robert J. Zawadzki, David D. Sampson
Summary: The visualization and assessment of retinal microvasculature are crucial in the study and treatment of eye and systemic diseases. However, the current methods and analysis of OCTA imaging lack consistency, hindering progress. This paper proposes steps to standardize OCTA imaging, including imaging protocols, data analysis methods, metrics, reporting, and clinical practice, to address these limitations.
LIGHT-SCIENCE & APPLICATIONS
(2022)
Article
Biochemical Research Methods
Loretta Scolaro, Dirk Lorenser, Bryden C. Quirk, Rodney W. Kirk, Louisa A. Ho, Elizabeth Thomas, Jiawen Li, Christobel M. Saunders, David D. Sampson, Rebecca O. Fuller, Robert A. McLaughlin
Summary: This article introduces a multimodal imaging needle that can differentiate cell types through simultaneous optical coherence tomography (OCT) and fluorescence imaging. By conducting experiments on ER+ and ER- breast cancer cells, it is demonstrated that this miniaturized imaging probe can successfully image live cells and differentiate cancer cells.
JOURNAL OF BIOMEDICAL OPTICS
(2022)
Article
Engineering, Electrical & Electronic
Anand Arangath, Kai Neuhaus, Sergey Alexandrov, Martin Leahy
Summary: This study introduces a multiple reference optical coherence tomography technique that significantly improves imaging range and enables imaging of highly scattering biological samples. The signal properties are analyzed through numerical simulations and measurements, and the signal degradation caused by birefringence in the sample is discussed.
IEEE PHOTONICS JOURNAL
(2022)
Article
Engineering, Electrical & Electronic
Karol Karnowski, Gavrielle Untracht, Michael Hackmann, Onur Cetinkaya, David Sampson
Summary: This study explores the use of a side-viewing probe combining graded-index fiber and a ball lens, which demonstrates superior performance in optical imaging. Through detailed analysis and modeling, it is shown that this probe design offers excellent performance across various numerical apertures and opens up new possibilities for broader imaging applications.
IEEE PHOTONICS JOURNAL
(2022)
Article
Biochemical Research Methods
Rajib Dey, Sergey Alexandrov, Peter Owens, Jack Kelly, Sine Phelan, Martin Leahy
Summary: This paper presents a non-invasive imaging method for skin tissue based on nanosensitive OCT technology, which can detect structural changes in the skin at the nanoscale. It is of great significance for the diagnosis and clinical management of skin diseases.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Multidisciplinary Sciences
Gavrielle R. Untracht, Nikolaos Dikaios, Abdullah K. Durrani, Mariam Bapir, Marinko V. Sarunic, David D. Sampson, Christian Heiss, Danuta M. Sampson
Summary: This pilot study evaluates OCTA-derived microvascular parameters in healthy individuals and patients with diabetes. It establishes reference values for different regions of the body and identifies potential biomarkers for diabetes.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Ryan McAuley, A. Nolan, A. Curatolo, S. Alexandrov, F. Zvietcovich, A. Varea Bejar, S. Marcos, M. Leahy, J. S. Birkenfeld
Summary: A co-axial acoustic-based optical coherence vibrometry probe (CoA-OCV) was developed for quantifying vibro-acoustic resonance in biological tissues, showing potential for future OCT-V studies. Experiment results demonstrated the relationship between resonance frequencies and factors such as intraocular pressure, Young's Modulus, and phantom thickness in corneal models and rabbit corneas. The study highlighted the predominant impact of Young's Modulus and provided insights into the frequency shift and full width at half maximum of resonance modes.
SCIENTIFIC REPORTS
(2022)
Article
Biology
Mariam Bapir, Gavrielle R. R. Untracht, Julie E. A. Hunt, John H. H. McVey, Jenny Harris, Simon S. S. Skene, Paola Campagnolo, Nikolaos Dikaios, Ana Rodriguez-Mateos, David D. D. Sampson, Danuta M. M. Sampson, Christian Heiss
Summary: Endothelial function in the femoral artery and brachial artery is similarly affected by age in healthy adults, leading to an increased susceptibility to peripheral artery disease in old age.
Article
Oncology
Rachel Lloyd, Sarah Pirikahu, Jane Walter, Gemma Cadby, Ellie Darcey, Dilukshi Perera, Martha Hickey, Christobel Saunders, Karol Karnowski, David D. Sampson, John Shepherd, Lothar Lilge, Jennifer Stone
Summary: This study examines the acceptability and performance of two alternative breast density measures, Optical Breast Spectroscopy (OBS) and Dual X-ray Absorptiometry (DXA), in women aged 18-40. The results showed that over 93% of participants deemed OBS and DXA to be acceptable. The correlation between OBS-%water + collagen and %FGV was 0.48, and age and BMI were associated with breast density measures.
BRITISH JOURNAL OF CANCER
(2023)
Article
Biochemical Research Methods
Anand Arangath, Niamh Duffy, Sergey Alexandrov, Soorya James, Kai Neuhaus, Mary Murphy, Martin Leahy
Summary: MSCs have shown therapeutic potential for regenerating damaged tissues in various diseases. Nanosensitive optical coherence tomography is a promising imaging modality for tracking MSCs and providing functional information about cell therapies.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Optics
Anand Arangath, Kai Neuhaus, Sergey Alexandrov, Martin Leahy
Summary: Digitally balanced detection (DBD) has been studied in Fourier domain optical coherence tomography systems to improve sensitivity. We report a technical advancement in a multiple reference optical coherence tomography (MR-OCT) system with the implementation of the DBD technique. The application of the DBD scheme for MR-OCT achieves an average sensitivity improvement of 5 +/- 0.5 dB over its analogue balanced detection counterpart. We also show that DBD improves the contrast on images of Scotch tapes and mouse eyes.
Review
Multidisciplinary Sciences
Brett E. Bouma, Johannes F. de Boer, David Huang, Ik-Kyung Jang, Taishi Yonetsu, Cadman L. Leggett, Rainer Leitgeb, David D. Sampson, Melissa Suter, Ben J. Vakoc, Martin Villiger, Maciej Wojtkowski
Summary: This article introduces the principles, applications, and future prospects of optical coherence tomography (OCT). By explaining the principles and origins of signals for different instrument configurations, as well as image processing and interpretation methods, the article comprehensively analyzes the application of OCT in biological and medical imaging.
NATURE REVIEWS METHODS PRIMERS
(2022)
Article
Biochemistry & Molecular Biology
Mariam Bapir, Gavrielle R. Untracht, Debbie Cooke, John H. McVey, Simon S. Skene, Paola Campagnolo, Martin B. Whyte, Nikolaos Dikaios, Ana Rodriguez-Mateos, David D. Sampson, Danuta M. Sampson, Christian Heiss
Summary: This study found that individuals with type 2 diabetes mellitus (T2DM) exhibit greater impairment in femoral artery endothelial function and dermal microcirculation. The consumption of cocoa flavanol (CF) improves endothelial function in both healthy individuals and those with T2DM, who are at increased risk of developing lower extremity peripheral artery disease (PAD) and foot ulcers.
