4.6 Article

Sensitive molecular binding assay using a photonic crystal structure in total internal reflection

Journal

OPTICS EXPRESS
Volume 16, Issue 16, Pages 11741-11749

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OPTICAL SOC AMER
DOI: 10.1364/OE.16.011741

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  1. NCRR NIH HHS [1 R21 RR021893, R21 RR021893-01, R21 RR021893] Funding Source: Medline

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A novel optical sensor for label-free biomolecular binding assay using a one-dimensional photonic crystal in a total-internal-reflection geometry is proposed and demonstrated. The simple configuration provides a narrow optical resonance to enable sensitive measurements of molecular binding, and at the same time employs an open interface to enable real-time measurements of binding dynamics. Ultrathin aminopropyltriethoxysilane/glutaraldehyde films adsorbed on the interface were detected by measuring the spectral shift of the photonic crystal resonance and the intensity ratio change in a differential reflectance measurement. A detection limit of 6 x 10(-5) nm for molecular layer thickness was obtained, which corresponds to a detection limit for analyte adsorption of 0.06 pg/mm(2) or a refractive index resolution of 3 x 10(-8) RIU; this represents a significant improvement relative to state-of-the-art surface-plasmon-resonance-based systems. (c) 2008 Optical Society of America.

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