Journal
OPTICS EXPRESS
Volume 16, Issue 3, Pages 2168-2175Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.16.002168
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Funding
- Howard Hughes Medical Institute Funding Source: Medline
- NCRR NIH HHS [UL1 RR024146] Funding Source: Medline
- NHLBI NIH HHS [HL78615] Funding Source: Medline
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We present a novel scheme to simultaneously detect coherent anti-Stokes Raman scattering ( CARS) microscopy signals in the forward ( F) and backward (epi-E) direction with a single avalanche photodiode (APD) detector using time-correlated single photon counting (TCSPC). By installing a mirror at a well-defined distance above the sample the forward-scattered F-CARS signal is reflected back into the microscope objective leading to spatial overlap of the F and E-CARS signals. Due to traveling an additional distance the F-CARS signal is time delayed relative to the E-CARS signal. TCSPC then allows for the two signals to be resolved in the time domain. This results in an efficient, simple, and compact method of CARS signal detection. We demonstrate this technique by analyzing forward and backward CARS signals obtained by imaging living adipocyte cells derived from human mesenchymal stem cells. (c) 2008 Optical Society of America.
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