Article
Agronomy
Haiming Han, Xinyuan Ma, Zhen Wang, Kai Qi, Wenjing Yang, Weihua Liu, Jinpeng Zhang, Shenghui Zhou, Yuqing Lu, Xinming Yang, Xiuquan Li, Lihui Li
Summary: In this study, chromosome 5P from A. cristatum was found to induce various chromosomal structural variations in a common wheat background. These variations disrupted chromosome pairing and meiotic behavior, leading to aberrant chromosome behavior. Thirteen genes involved in DNA repair or chromatin remodeling were upregulated in wheat-A. cristatum 5P addition lines, suggesting the involvement of chromosome 5P in meiotic double-strand break repair. These findings provide a new and efficient tool for inducing wheat-alien chromosome translocations and generating new germplasm.
Article
Agronomy
Kai Qi, Haiming Han, Jinpeng Zhang, Shenghui Zhou, Xiuquan Li, Xinming Yang, Weihua Liu, Yuqing Lu, Lihui Li
Summary: Agropyron cristatum, an important wild relative of wheat, may carry genes for increased grain weight and width on its 6P chromosome. The development of Robertsonian translocation lines could promote the utilization of the 6P chromosome of Agropyron cristatum in wheat improvement.
MOLECULAR BREEDING
(2021)
Article
Agriculture, Multidisciplinary
Xu Shi-rui, Jiang Bo, Han Hai-ming, Ji Xia-jie, Zhang Jin-peng, Zhou Sheng-hui, Yang Xin-ming, Li Xiu-quan, Li Li-hui, Liu Wei-hua
Summary: This study evaluated agronomic traits and genetic analysis of wheat-A. cristatum translocation lines. It was found that certain translocation fragments could affect leaf length, leaf width, spikelet density, and plant height of wheat.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Plant Sciences
Pilar Prieto, Carmen Palomino, Zuny Cifuentes, Adoracion Cabrera
Summary: The Ph1 locus does not affect chromosome associations between Agropyron and wheat chromosomes, as there were no interspecific chromosome associations without the Ph1 locus. The 5P and 6P Agropyron chromosomes do not suppress the Ph1 locus. Wheat univalents in metaphase I suggest potential genes carried by Agropyron chromosomes may influence wheat homologous chromosome associations, which do not interact with the Ph1 locus.
Article
Biochemistry & Molecular Biology
Zhi Zhang, Shenghui Zhou, Weihua Liu, Liqiang Song, Jinpeng Zhang, Haiming Han, Xinming Yang, Yida Lin, Xiuquan Li, Lihui Li
Summary: This study identified three disease resistance-related transcripts from Agropyron cristatum 6PS (0.81-1.00) through molecular marker analysis and analyzed the progenies of the translocation line WAT655 to observe the introgression between the A. cristatum P genome and the wheat genome. Additionally, KASP markers of the three genes were developed to detect and acquire breeding lines with resistance and ideal agronomic traits.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agronomy
Alejandro Copete-Parada, Carmen Palomino, Adoracion Cabrera
Summary: The study utilized gametocidal chromosomes and pairing homologous gene mutants to induce translocations between wheat and Agropyron cristatum. A total of 19 translocations were identified and characterized using molecular markers, with the highest frequency observed for chromosome 4P. These translocation lines are valuable for identifying A. cristatum related genes and provide new genetic resources for wheat improvement.
Article
Agronomy
Yida Lin, Shenghui Zhou, Xuezhong Liang, Bing Han, Junli Yang, Baojin Guo, Jinpeng Zhang, Haiming Han, Weihua Liu, Xinming Yang, Xiuquan Li, Lihui Li
Summary: In this study, a novel translocation line, WAT650l, was identified, which can increase both grain number per spike and thousand-grain weight in wheat. Cytological analysis and molecular marker analysis revealed that WAT650l was a 5BL.5BS-6PL translocation line. This finding provides valuable germplasm resources for wheat breeding.
Article
Agronomy
Shirui Xu, Xiajie Ji, Suli Sun, Haiming Han, Jinpeng Zhang, Shenghui Zhou, Xinming Yang, Xiuquan Li, Lihui Li, Weihua Liu
Summary: In this study, new wheat-A. cristatum 2P translocation lines were induced using ionizing radiation, resulting in 10 plants with reduced 2P chromatin and 6 plants without visible 2P chromosomal fragments. Moreover, four lines were resistant to both powdery mildew and leaf rust, while two lines were resistant only to leaf rust.
MOLECULAR BREEDING
(2022)
Article
Biochemistry & Molecular Biology
Wenjing Yang, Haiming Han, Baojin Guo, Kai Qi, Jinpeng Zhang, Shenghui Zhou, Xinming Yang, Xiuquan Li, Yuqing Lu, Weihua Liu, Xu Liu, Lihui Li
Summary: Wild relatives of wheat serve as vital gene pools for broadening wheat's genetic basis. Chromosome rearrangements and genomic variation in alien chromosomes are common occurrences, and knowledge of the genetic variation between alien homologous chromosomes is essential for discovering and utilizing alien genes. In this study, two wheat-A. cristatum 6P addition lines exhibited significant differences in various traits and genomic analysis revealed several differences in the 6P chromosomes of these two lines. The study also showed that the variance in genes on the 6P chromosome is closely related to phenotypic differences, providing important guidance for cloning desirable genes and their utilization in wheat improvement.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Cuili Pan, Qingfeng Li, Haiming Han, Jinpeng Zhang, Shenghui Zhou, Xinming Yang, Xiuquan Li, Lihui Li, Weihua Liu
Summary: In this study, a wheat-A. cristatum derivative with a pair of 5P chromosomes and a pair of 2P chromosomes was identified. The 5P chromosome was found to weaken the homologous pairing of wheat chromosomes and promote the pairing between homoeologous chromosomes, providing new materials for studying the mechanism of alien gene affecting chromosome behavior in wheat.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Adoracion Cabrera, Rafael Porras, Carmen Palomino, Josefina Carmen Sillero
Summary: In this study, two new translocations of Agropyron cristatum were obtained by engineering chromosome arm 1PS, which showed resistance to leaf rust. One translocation replaced a proximal segment of 1PS chromatin, while the other replaced a distal segment and introduced wheat storage protein loci.
Article
Plant Sciences
Mingming Yao, Xinhua Wang, Jiaohui Long, Shuangyu Bai, Yuanyuan Cui, Zhaoyi Wang, Caixia Liu, Fenglou Liu, Zhangjun Wang, Qingfeng Li
Summary: This study investigated the molecular mechanism of powdery mildew resistance in the wheat-A. cristatum translocation line WAT2020-17-6 through transcriptome sequencing and weighted gene co-expression network analysis. The candidate hub genes were identified, laying a foundation for the exploration of resistance genes in A. cristatum for powdery mildew-resistant breeding in wheat.
Article
Agronomy
Ziyuan Kuang, Xiajie Ji, Shirui Xu, Haiming Han, Jinpeng Zhang, Shenghui Zhou, Xinming Yang, Xiuquan Li, Lihui Li, Weihua Liu
Summary: In this study, the wheat-A. cristatum 3P addition line I-27 and 3P(3B) substitution line I-15 were identified for the first time, which have excellent genetic characteristics and can be used for the genetic improvement of wheat. These materials lay the foundation for the utilization of excellent genes from the A. cristatum chromosome 3P.
Article
Plant Sciences
Yun-Feng Xu, Fei-Fei Ma, Jin-Peng Zhang, Hong Liu, Li-Hui Li, Diao-Guo An
Summary: In this study, a recombinant inbred line (RIL) population derived from the wheat-Agropyron cristatum germplasm PB3228 was developed and a high-density genetic map was constructed. Five environmentally stable quantitative trait loci (QTLs) related to grain number and spikelet number per spike were identified, with PB3228 contributing significantly to these QTLs.
Article
Biochemistry & Molecular Biology
Xiajie Ji, Taiguo Liu, Shirui Xu, Zongyao Wang, Haiming Han, Shenghui Zhou, Baojin Guo, Jinpeng Zhang, Xinming Yang, Xiuquan Li, Lihui Li, Weihua Liu
Summary: In this study, the specific Agropyron cristatum resistance genes and related regulatory pathways in the wheat-A. cristatum addition line II-9-3 were identified through comparative transcriptome analysis. The differentially expressed genes (DEGs) in II-9-3 were found to be associated with plant-pathogen interaction, signaling pathways, plant hormone signal transduction, and other pathways. Additionally, several defense-related genes from A. cristatum were identified. These findings have important implications for the utilization of innovative resources for leaf rust resistance in wheat breeding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Xueling Ye, Jian Li, Yukun Cheng, Fangjie Yao, Li Long, Can Yu, Yuqi Wang, Yu Wu, Jing Li, Jirui Wang, Qiantao Jiang, Wei Li, Jian Ma, Yuming Wei, Youliang Zheng, Guoyue Chen
Article
Biotechnology & Applied Microbiology
Xueling Ye, Jian Li, Yukun Cheng, Fangjie Yao, Li Long, Yuqi Wang, Yu Wu, Jing Li, Jirui Wang, Qiantao Jiang, Houyang Kang, Wei Li, Pengfei Qi, Xiujin Lan, Jian Ma, Yaxi Liu, Yunfeng Jiang, Yuming Wei, Xianming Chen, Chunji Liu, Youliang Zheng, Guoyue Chen
Article
Biotechnology & Applied Microbiology
Xueling Ye, Haiyan Hu, Hong Zhou, Yunfeng Jiang, Shang Gao, Zhongwei Yuan, Jiri Stiller, Chengwei Li, Guoyue Chen, Yaxi Liu, Yuming Wei, You-Liang Zheng, You-Gan Wang, Chunji Liu
Summary: Consistent differences were not detected in subgenome between artificial and natural genotypes of polyploid species, indicating that subgenome asymmetry may largely reflect differences between diploid donors rather than changes during polyploid evolution. Subgenome asymmetry was observed in genotypes before and after polyploidization events, suggesting a strong influence of diploid donors on defining the changes during polyploid evolution.