Journal
TETRAHEDRON LETTERS
Volume 56, Issue 23, Pages 3639-3642Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tetlet.2015.01.165
Keywords
Oligonucleotide synthesis; Light-activation; Photocaged nucleotides; Immune response; Toll-like receptors
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Funding
- NIGMS NIH HHS [R01 GM079114] Funding Source: Medline
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Bacterial and viral CpG oligonculeotides are unmethylated cytosine-phosphate-guanosine dinucleotide sequences and trigger an innate immune response through activation of the toll-like receptor 9 (TLR9). We have developed synthetic photocaged CpGs via site-specific incorporation of nitropiperonyloxymethyl (NPOM)-caged thymidine residues. These oligonucleotides enable the optical control of TLR9 function and thereby provide light-activation of an immune response. We provide a proof-of-concept model by applying a reporter assay in live cells and by quantification of endogenous production of interleukin 6. (C) 2015 Elsevier Ltd. All rights reserved.
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