Article
Optics
Daewoon Seong, Won Ki, Pilun Kim, Jaeyul Lee, Sangyeob Han, Soojin Yi, Hong Kyun Kim, Mansik Jeon, Jeehyun Kim
Summary: This study developed a virtual intraoperative OCT angiography integrated surgical microscope, which can simultaneously visualize the morphological tissue structure and microvasculature data of the surgical region, demonstrating its potential application in in vivo imaging and simulated surgeries of small animals.
OPTICS AND LASERS IN ENGINEERING
(2022)
Editorial Material
Multidisciplinary Sciences
Tinsley H. Davis
Summary: Optical coherence tomography (OCT), a noninvasive imaging method that measures the delay in return of reflected light, has become a standard of care in ophthalmology and has various applications in medical and industrial fields. James Fujimoto, David Huang, and Eric Swanson won the LaskerDeBakey Clinical Medical Research Award for their invention and development of OCT, and in an interview, they discussed the technology's development and future applications.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Optics
Zoya Alam, Raju Poddar
Summary: Zebrafish, as a vertebrate model, is widely used in various fields. This study demonstrates the use of optical coherence tomography and optical coherence tomography angiography to image the developing zebrafish, allowing for detailed visualization of microstructures and microvasculature.
OPTICS AND LASERS IN ENGINEERING
(2022)
Article
Multidisciplinary Sciences
Aldo Vagge, Paolo Corazza, Lorenzo Ferro Desideri, Paola Camicione, Giulia Agosto, Roberta Vagge, Calevo Maria Grazia, Adriano Carnevali, Giuseppe Giannaccare, Massimo Nicolo, Carlo Enrico Traverso
Summary: Patients with NF1 exhibit lower macular RNFL and retinal thickness, along with higher vascular flow area in the SCP. Compared to control subjects, the flow area in the second choroidal layer is also lower in NF1 patients. OCTA is a potentially valuable tool for detecting early ocular abnormalities in NF1.
Article
Ophthalmology
Aylin Karalezli, Sema Tamer Kaderli, Sabahattin Sul
Summary: A study compared the macular microvasculature differences in cases with idiopathic macular hole after vitrectomy using internal limiting membrane removal or temporal inverted ILM flap technique. The results showed that the temporal IFT causes fewer VD changes, especially in the DCP, compared to complete ILM removal.
Article
Optics
Yue Zhang, Wanrong Gao, Chenxia Xie
Summary: This paper proposes a new method to remove noise in blood flow images using the spatial frequency information of motion noise. The effectiveness of the adaptive denoising algorithm is verified through finger blood flow imaging. It is found that applying the algorithm improves the signal-to-noise ratio and contrast-to-noise ratio of OCTA images.
Article
Optics
Huakun Li, Kaiyuan Liu, Tongtong Cao, Lin Yao, Ziyi Zhang, Xiaofeng Deng, Chixin Du, Peng Li
Summary: Motion contrast optical coherence tomography angiography (OCTA) aims to identify dynamic flow signals accurately. An intermediate region with mixed distribution of dynamic and static scatterers often occurs in practice, degrading vascular contrast and connectivity. A new OCTA classifier, SID-OCTA, was developed and experimentally validated for improved performance.
Article
Medicine, General & Internal
Thanh-Tin P. Nguyen, Shuibin Ni, Guangru Liang, Shanjida Khan, Xiang Wei, Alison Skalet, Susan Ostmo, Michael F. Chiang, Yali Jia, David Huang, Yifan Jian, J. Peter Campbell
Summary: OCT has changed the standard of care for macular diseases in adults, but commercially available systems have a narrow FOV limiting their application to peripheral pathologies; OCT-based evaluation can improve diagnosis of all types of retinal detachment.
FRONTIERS IN MEDICINE
(2022)
Article
Ophthalmology
Matteo Menean, Riccardo Sacconi, Beatrice Tombolini, Federico Fantaguzzi, Francesco Bandello, Giuseppe Querques
Summary: This study aimed to detect retinal neovascularization elsewhere (NVE), of the optic disc (NVD) and intraretinal microvascular abnormalities (IRMA) in treatment naive diabetic retinopathy (DR) using wide-field colour fundus photography (WF CFP), wide-field spectral-domain optical coherence tomography angiography (OCTA) and the combination of WF CFP and OCTA through overlay software. The results showed that OCTA had high levels of agreement in detecting NVE, NVD and IRMA, and combining WF CFP and OCTA further improved the detection of NVE and NVD.
Article
Computer Science, Artificial Intelligence
Wenwen Kong, Pengxiao Zang, Sijie Niu, Dengwang Li
Summary: This paper proposes an automated registration method for multi-modality retinal fundus photographs based on the directional vessel skeleton, aiming to provide clinicians with multi-modality information to diagnose retinal diseases or make treatment decisions. The method shows advantageous performance in both registration success rate and accuracy when evaluated with various types of fundus photographs in the macular regions of patients with diseases.
IET IMAGE PROCESSING
(2021)
Article
Biochemical Research Methods
Fan Fan, Jisheng Zhang, Lianqing Zhu, Zongqing Ma, Jiang Zhu
Summary: A deep learning-based segmentation method was proposed to improve the image quality and enhance the vasculature clarity in OCTA. By evaluating PSNR and CNR values, the study showed that the method performed well in enhancing the quality and accuracy of vascular network projections.
JOURNAL OF BIOPHOTONICS
(2021)
Article
Immunology
Christina Noll, Michael Hiltensperger, Lilian Aly, Rebecca Wicklein, Ali Maisam Afzali, Christian Mardin, Christiane Gasperi, Achim Berthele, Bernhard Hemmer, Thomas Korn, Benjamin Knier
Summary: This study found that rarefication of the retinal vasculature during RRMS is associated with higher frequencies of activated B cells and higher levels of pro-inflammatory cytokines in the cerebrospinal fluid. Furthermore, vessel loss in the retina is linked to future disability worsening.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biology
Ines Lopez-Cuenca, Elena Salobrar-Garcia, Lorena Elvira-Hurtado, Jose A. Fernandez-Albarral, Lidia Sanchez-Puebla, Juan J. Salazar, Jose M. Ramirez, Ana Ramirez, Rosa de Hoz
Summary: The retinal changes measured by optical coherence tomography (OCT) and OCT-angiography (OCTA) may serve as potential biomarkers of preclinical Alzheimer's disease. More unified methodology, rigorous inclusion criteria, and longer longitudinal studies are needed to assess the evolution of subjects and determine the reliability of these retinal changes as biomarkers for early diagnosis.
Article
Medicine, General & Internal
Lisa Toto, Maria Ludovica Ruggeri, Federica Evangelista, Chiara Trivigno, Rossella D'Aloisio, Chiara De Nicola, Pasquale Viggiano, Emanuele Doronzo, Marta Di Nicola, Annamaria Porreca, Rodolfo Mastropasqua
Summary: This study investigates the parameters of optical coherence tomography (OCT) and OCT angiography (OCTA) in patients with neovascular age-related macular degeneration (nAMD) and macular neovascularization (MNV) types 1, 2, and 3. The results showed significant differences in CVI, CMT, and ORCCFA between MNV 1 and the other two groups. CVI was significantly different between MNV 1 and healthy controls and between MNV 1 and MNV 2. ORCCFA and CMT were significantly different between MNV 1 and MNV 2. There was no statistically significant difference in subfoveal choroidal thickness (SFCT) among the three groups.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemical Research Methods
Chaojiang Yang, Jiang Zhu, Lianqing Zhu, Fan Fan, Zongqing Ma, Fan Zhang
Summary: This study introduces three optimized OCTA algorithms that allow for faster quantification of blood flow velocities with equivalent image quality compared to traditional algorithms.
JOURNAL OF BIOPHOTONICS
(2022)
Article
Biochemical Research Methods
Lijie Zhang, Rongyao Dong, Robert J. Zawadzki, Pengfei Zhang
Summary: Optoretinogram, utilizing OCT to measure retinal functions, is a potentially useful tool for quantifying retinal health alterations. This study improved accuracy by collecting volumetric data and developing a 3D registration approach, revealing novel functional signals and enabling monitoring of local and global functional changes in rodent eyes.
JOURNAL OF BIOPHOTONICS
(2022)
Review
Optics
Danuta M. Sampson, Adam M. Dubis, Fred K. Chen, Robert J. Zawadzki, David D. Sampson
Summary: The visualization and assessment of retinal microvasculature are crucial in the study and treatment of eye and systemic diseases. However, the current methods and analysis of OCTA imaging lack consistency, hindering progress. This paper proposes steps to standardize OCTA imaging, including imaging protocols, data analysis methods, metrics, reporting, and clinical practice, to address these limitations.
LIGHT-SCIENCE & APPLICATIONS
(2022)
Article
Medicine, Research & Experimental
Sook Hyun Chung, Tzu-Ni Sin, Brian Dang, Taylor Ngo, Therlinder Lo, Daniella Lent-Schochet, Ratheesh K. Meleppat, Robert J. Zawadzki, Glenn Yiu
Summary: This study compares the effectiveness of using single versus paired gRNAs in the CRISPR-Cas9 system to target the Vegfa gene. Paired gRNAs increased gene ablation rates in human cells but did not enhance VEGF suppression in mouse eyes. Using two gRNAs for genome editing may increase the risk of off-target effects.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Ophthalmology
Ratheesh K. Meleppat, Christopher R. Fortenbach, Yifan Jian, Esteban Soto Martinez, Karen Wagner, Bobeck S. Modjtahedi, Monica J. Motta, Deepa L. Ramamurthy, Ivan R. Schwab, Robert J. Zawadzki
Summary: In vivo evaluation of the structural morphology and vascular plexuses of the neurosensory retina and choroid across vertebrate species was performed using SS-OCT and SS-OCTA imaging. The results showed that the retinal morphology and vascular plexuses varied among different species. These methods can be used to evaluate retinal diseases in various species.
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2022)
Article
Optics
Kari V. Vienola, Denise Valente, Robert J. Zawadzki, Ravi S. Jonnal
Summary: Optoretinography (ORG) is a noninvasive and objective tool for testing neural function in the retina. It has the potential to transform ophthalmic care and clinical trials of therapeutics for visual function. The article presents an alternative approach that monitors the velocity of retinal features instead of tracking their positions, reducing the complexity and cost of position-based methods.
Article
Optics
Soohyun Lee, Stacey S. Choi, Ratheesh K. Meleppat, Robert J. Zawadzki, Nathan Doble
Summary: This article describes a high-speed, adaptive optics partially confocal multi-spot ophthalmoscope (AO-pcMSO) that utilizes a digital micromirror device (DMD) in the illumination channel and a fast 2D CMOS camera. The camera is synchronized with the DMD to project multiple AO-corrected spots onto the human retina simultaneously. Spatial filtering on each raw retinal image acts as an array virtual pinholes. The parallel projection scheme achieves a frame acquisition rate of 250 fps and improves contrast by 2-3 fold compared to a standard flood illumination architecture. Partially confocal images of the human retina reveal cone and rod photoreceptors at various retinal eccentricities.
Article
Biochemical Research Methods
Pengfei Zhang, Daniel J. Wahl, Jacopo Mocci, Eric B. Miller, Stefano Bonora, Marinko Sarunic, Robert J. Zawadzki
Summary: Optical coherence tomography (OCT) and scanning laser ophthalmoscopy (SLO), invented in the 1980s, have greatly advanced in vivo retinal diagnostics in ophthalmology clinics and vision science research. Adaptive optics (AO) technology improves the resolution and sensitivity of SLO and OCT systems by correcting ocular aberrations. Integrating OCT into an existing mouse retinal AO-SLO system allows for multi-modal AO-enhanced imaging of the living mouse eye with high acquisition speed and resolution.
BIOMEDICAL OPTICS EXPRESS
(2023)
Proceedings Paper
Instruments & Instrumentation
Hang Chan Jo, Robert J. Zawadzki, Dae Yu Kim
Summary: Adaptive optics (AO) is a technique that compensates for aberration in optical imaging systems. Previous research has focused on improving AO performance through the use of spherical mirrors and off-axis designs.
OPTICAL SYSTEM ALIGNMENT, TOLERANCING, AND VERIFICATION XIV
(2022)
Meeting Abstract
Ophthalmology
Robert J. Zawadzki, Ratheesh K. Meleppat, Denise Valente, Soohyun Lee, Kari V. Vienola, Pengfei Zhang, Ravi S. Jonnal, Nathan Doble
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Meeting Abstract
Ophthalmology
Kari V. Vienola, Robert J. Zawadzki, Ravi S. Jonnal
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Meeting Abstract
Ophthalmology
Subeen Park, Hang Chan Jo, Kyoung Min Lee, Da Young Song, Robert J. Zawadzki, Dae Yu Kim, Seok Hwan Kim, Jae Hun Kim
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Meeting Abstract
Ophthalmology
Ewelina Pijewska, Kari V. Vienola, Michal Meina, Pengfei Zhang, Ratheesh K. Meleppat, Maciej Szkulmowski, Ravi S. Jonnal, Robert J. Zawadzki
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Meeting Abstract
Ophthalmology
Christopher Loh, Pengfei Zhang, Ratheesh K. Meleppat, Oscar Ramos-Soto, Sandra E. Balderas-Mata, Robert J. Zawadzki, Susanna S. Park
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Meeting Abstract
Ophthalmology
Ratheesh K. Meleppat, Kaitryn Ronning, Sarah J. Karlen, J. KarlenMarie E. Burns, Robert J. Zawadzki
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Meeting Abstract
Ophthalmology
Jessicca Cho, Pengfei Zhang, Sarah J. Karlen, Nicholas Marsh-Armstrong, Anna La Torre, Robert J. Zawadzki
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)