4.4 Article

Invadopodia Formation by Bladder Tumor Cells

Journal

ONCOLOGY RESEARCH
Volume 19, Issue 2, Pages 85-92

Publisher

COGNIZANT COMMUNICATION CORP
DOI: 10.3727/096504010X12875107808008

Keywords

Invadopodia; Bladder tumor; Tumor invasion

Categories

Funding

  1. Japan Society for the Promotion of Science [22390301, 22570131]
  2. Ministry of Education, Culture, Sports, Science and Technology of Japan [21791483, 21791484]

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major cause of death in patients with bladder tumors is recurrence with metastasis. Bladder tumor metastasis is largely dependent upon the invasive capacity of tumor cells. Tumor cell invasion is mainly mediated by actin-rich protrusive membrane structures called invadopodia. The formation of invadopodia was observed in various types of invasive tumors such as breast cancer and melanomas. However, invadopodia formation so far has not been described in bladder tumor cells. We here report that human bladder tumor cells form functionally active invadopodia. By using a confocal laser scanning microscope, we demonstrated that invasive bladder tumor cell lines, YTS-1 and T24, with high Matrigel degradation activity form invadopodia but that noninvasive bladder tumor cell lines, RT4 and KK-47, form no detectable invadopodia. Invadopodia formed by YTS-1 cells had the ability to secrete matrix metalloproteases and degrade extracellular matrix to invade surrounding areas. Moreover, we observed that primary tumor cells obtained from patients with invasive bladder tumors also form invadopodia, validating the results from bladder tumor cell lines. Our results provide evidence that invasive human bladder tumor cells form invadopodia for tumor invasion.

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