Article
Sport Sciences
Aaron L. Slusher, Jeongjin J. J. Kim, Mark Ribick, Andrew T. Ludlow
Summary: Aerobic exercise maintains telomere length by increasing hTERT gene expression and altering hTERT alternative splicing, which can improve the function and health of the organism.
MEDICINE & SCIENCE IN SPORTS & EXERCISE
(2022)
Article
Biotechnology & Applied Microbiology
Yiming Wang, Linfei Song, Mengfei Ning, Jiaxiang Hu, Han Cai, Weitao Song, Daoqing Gong, Long Liu, Jacqueline Smith, Huifang Li, Yinhua Huang
Summary: This study identifies alternative splicing events (ASEs) related to fatty liver formation in overfed ducks using full-length transcripts and Illumina RNA-seq data. Differential expression of genes involved in lipid metabolism due to ASEs was observed. This study provides insights into the molecular mechanisms of non-pathogenic fatty liver.
Article
Cell Biology
Jagyeong Oh, Davide Pradella, Changwei Shao, Hairi Li, Namjeong Choi, Jiyeon Ha, Sonia Ruggiero, Xiang-Dong Fu, Xuexiu Zheng, Claudia Ghigna, Haihong Shen
Summary: Aberrant alternative splicing in breast cancer is linked to disease progression, metastasis, and patient survival. High-metastatic breast cancer cells exhibit different AS choices in genes related to cancer progression compared to low-metastatic cells. Analysis reveals novel AS features in metastatic breast tumors.
Article
Agriculture, Multidisciplinary
He-nan Su, Yu-xiang Yuan, Shuang-juan Yang, Xiao-chun Wei, Yan-yan Zhao, Zhi-yong Wang, Liu-yue Qin, Zhi-yuan Yang, Liu-jing Niu, Lin Li, Xiao-wei Zhang
Summary: In this study, PacBio RS II SMRT sequencing was used to analyze the full-length transcriptomes of a clubroot-resistant Chinese cabbage variety. The results identified a large number of genes related to clubroot resistance, providing valuable resources for basic research on clubroot resistance in Chinese cabbage.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Oncology
Jagyeong Oh, Davide Pradella, Yoonseong Kim, Changwei Shao, Hairi Li, Namjeong Choi, Jiyeon Ha, Anna Di Matteo, Xiang-Dong Fu, Xuexiu Zheng, Claudia Ghigna, Haihong Shen
Summary: Aberrant alternative splicing (AS) regulation is crucial in breast cancer development, progress, and resistance to therapy. Our study identified dysregulated AS events in breast cancer cells, shedding light on the molecular mechanisms underlying malignant transformation. Analysis revealed that aberrantly regulated AS events in cancer tissues encode for regions with high flexibility and potential for modifications, impacting breast cancer biology.
Article
Biochemistry & Molecular Biology
Hiroki Ura, Sumihito Togi, Yo Niida
Summary: Alternative splicing is a regulated process that can lead to disease. The SMARTer method provides full-length double-strand cDNA synthesis, but faces non-specific genomic DNA amplification issue. The improved SMARTer Capture method, incorporating target-specific capture with exon probes, can accurately detect more alternative splicing events and reduce non-specific amplification.
Article
Oncology
Jamal Elhasnaoui, Giulio Ferrero, Valentina Miano, Santina Cutrupi, Michele De Bortoli
Summary: This study reveals a novel mechanism of gene regulation mediated by estrogen receptor alpha in breast cancer cells, involving the control of RNA-binding protein expression and effects on isoform usage and alternative splicing. These regulatory mechanisms play a significant role in cell proliferation and epithelial-to-mesenchymal transition in breast cancer.
Article
Biology
Jayashree Kumar, Lela Lackey, Justin M. Waldern, Abhishek Dey, Anthony M. Mustoe, Kevin M. Weeks, David H. Mathews, Alain Laederach, Jonathan P. Staley
Summary: Splicing is a highly regulated process affected by multiple factors, making it challenging to predict the effects of mutations on pre-mRNA structure and function. In this study, a novel chemical probing strategy was used to visualize endogenous precursor and mature MAPT mRNA structures in cells, allowing for predictions of mutation consequences on pre-mRNA structure. Cryo-EM structures of the spliceosome were also analyzed to predict alternative splicing outcomes, achieving high accuracy. A beta-regression weighting framework incorporating splice site strength, RNA structure, and splicing regulatory elements was developed, enabling accurate prediction of the effects of known and newly discovered mutations on MAPT splicing.
Article
Oncology
Pihua Han, Jingjun Zhu, Guang Feng, Zizhang Wang, Yanni Ding
Summary: This study identified survival-associated AS events and signatures that can help predict the survival outcomes of patients with BRCA. Additionally, constructed SF-AS networks in BRCA can reveal the underlying regulatory mechanisms in BRCA.
Article
Oncology
Wenjing Xiao, Xin Chen, Xia Li, Kaiwen Deng, Huawei Liu, Jie Ma, Zhanhao Wang, Yonghe Hu, Jun Hou
Summary: The dysregulation of alternative splicing of the hTERT gene in pancreatic cancer has been found to be mediated by the splicing factor RBM10, resulting in the generation of a function-less isoform. The expression of RBM10 is negatively associated with the progression of PrCa, and manipulating its levels significantly affects cell proliferation both in vitro and in vivo. RBM10 promotes the exclusion of specific exons in the hTERT gene, leading to the production of a non-functional isoform.
AMERICAN JOURNAL OF CANCER RESEARCH
(2021)
Article
Multidisciplinary Sciences
Yun-Song Yang, Xi Jin, Qin Li, Yi-Yu Chen, Fenfang Chen, Hena Zhang, Ying Su, Yi Xiao, Gen-Hong Di, Yi-Zhou Jiang, Shenglin Huang, Zhi-Ming Shao
Summary: This study explored the transcription variation landscape in triple-negative breast cancers (TNBCs) and identified a crucial oncogenic transcript MARCO-TST, which is associated with tumor progression and poor prognosis in TNBC patients. MARCO-TST enhances the stability of HIF-1 alpha by interacting with PLOD2, leading to metabolic dysregulation and the formation of a hypoxic tumor microenvironment in TNBC.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Oncology
Hanting Liu, Xi Wang, Zheng Guo, Guanting Sun, Qiang Lv, Chao Qin, Lin Yuan, Yunyan Wang, Mulong Du, Meilin Wang, Zhengdong Zhang, Haiyan Chu
Summary: This study investigated the association between alternative splicing-related single nucleotide polymorphisms (SNPs) and susceptibility to bladder cancer. The results showed that SNP rs558814 in the lncRNA BCLET was associated with a decreased risk of bladder cancer.
Article
Biochemistry & Molecular Biology
Jamal Elhasnaoui, Giulio Ferrero, Valentina Miano, Lorenzo Franchitti, Isabella Tarulli, Lucia Coscujuela Tarrero, Santina Cutrupi, Michele De Bortoli
Summary: This study found that ESRP1 is overexpressed in breast cancer patients, particularly in luminal BCs, and is correlated with estrogen receptor alpha levels. Analysis of genome-binding profiles revealed that ER alpha regulates the expression of ESRP1 and ESRP2 genes. Knocking down ESRP1/2 led to dysregulation of 754 genes, including alterations in alternative splicing events involved in cell signaling, metabolism, cell growth, and EMT. Functional network analysis identified RAC1 as the hub node affected by ESRP1/2 silencing in ER alpha+ BCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
He Zhang, Baoai Han, Xingxing Han, Yuying Zhu, Hui Liu, Zhiyong Wang, Yanfen Cui, Ran Tian, Zicong Gao, Ruinan Tian, Sixin Ren, Xiaoyan Zuo, Jianfei Tian, Fei Zhang, Ruifang Niu
Summary: Recent evidence suggests that splicing factors (SFs) and alternative splicing (AS) play important roles in cancer progression. This study constructed SF-risk-models and AS-risk-models in different BRCA subtypes, providing potential multidimensional biomarkers for the diagnosis, prognosis, and treatment of BRCA. The functional roles of the selected SFs were found to be highly context-dependent among different BRCA subtypes.
FRONTIERS IN GENETICS
(2021)
Article
Genetics & Heredity
Youyuan Deng, Hongjun Zhao, Lifen Ye, Zhiya Hu, Kun Fang, Jianguo Wang
Summary: This study systematically analyzed the role of alternative splicing (AS) in breast cancer progression, showing its association with patient prognosis and immune cell infiltration. 13 AS events and 10 AS events were identified as independent prognostic factors for patients.
FRONTIERS IN GENETICS
(2021)
Meeting Abstract
Oncology
Zev A. Wainberg, Kohei Shitara, Eric Van Cutsem, Lucjan Wyrwicz, Keun Wook Lee, Iveta Kudaba, Marcelo Garrido, Hyun Cheol Cheol Chung, Jeeyun Lee, Hugo Raul Castro-Salguero, Wasat Mansoor, Maria Ignez Braghiroli, Nina Karaseva, Eray Goekkurt, Hironaga Satake, Joseph Chao, Uma Kher, Sukrut Shah, Pooja Bhagia, Josep Tabernero
JOURNAL OF CLINICAL ONCOLOGY
(2022)
Article
Oncology
Sun Young Rha, Su Kyung Song, Jiyeon Lee
Summary: The study aimed to explore the relationship between self-efficacy for coping with cancer and quality of life in patients with advanced gastric cancer. The results showed that self-efficacy for seeking and understanding medical information was the highest, while using spiritual coping was the lowest. Self-efficacy for maintaining activity and independence, accepting cancer/maintaining a positive attitude/making decisions significantly contributed to quality of life.
EUROPEAN JOURNAL OF ONCOLOGY NURSING
(2022)
Article
Oncology
Cristiane Bergerot, Sun Young Rha, Sumanta Pal, Piotr Koralewski, Daniil Stroyakovskiy, Boris Alekseev, Francis Parnis, Daniel Castellano, Jae Lyun Lee, Kaisa Sunela, Tudor Ciuleanu, Daniel Heng, Hilary Glen, Jinyi Wang, Lee Bennett, Janice Pan, Karen O'Hara, Javier Puente
Summary: This study focuses on preserving health-related quality of life during renal cell carcinoma treatment. The results showed that patients who received an 18 mg lenvatinib starting dose had better health-related quality of life scores and longer time to deterioration on most scales compared to those who received a 14 mg starting dose. These findings are important for guiding the treatment of renal cell carcinoma.
Article
Gastroenterology & Hepatology
Mitesh J. Borad, Li-Yuan Bai, Donald Richards, Kabir Mody, Joleen Hubbard, Sun Young Rha, John Soong, Daniel McCormick, Emmett Tse, Daniel O'Brien, Ahmad Bayat, Daniel Ahn, S. Lindsey Davis, Joon Oh Park, Do-Youn Oh
Summary: This study investigates the safety and efficacy of silmitasertib in combination with gemcitabine and cisplatin for the treatment of cholangiocarcinoma. The results show promising preliminary evidence of efficacy for the first-line treatment of locally advanced/metastatic cholangiocarcinoma.
Article
Oncology
D. V. T. Catenacci, Y-K Kang, H. H. Yoon, B. Y. Shim, S. T. Kim, D-Y Oh, A. Spira, S. Ulahannan, E. J. Avery, P. M. Boland, J. Chao, H. C. Chung, F. Gardner, S. J. Klempner, K-W Lee, S. C. Oh, J. Peguero, M. B. Sonbol, L. Shen, M. Moehler, J. Sun, D. Li, M. K. Rosales, H. Park
Summary: This study evaluated a novel treatment regimen, the combination of margetuximab and retifanlimab, in patients with HER2-positive metastatic gastric and gastroesophageal adenocarcinoma. The results showed that this regimen had a favorable toxicity profile and achieved a high objective response rate. However, the study was discontinued early due to recent advances in the treatment of this type of cancer.
Review
Oncology
Sun Young Rha, Hyun Cheol Chung
Summary: Gastric cancer is heterogeneous and can be characterized by HER2 overexpression and FGFR2 alterations. Combination therapy with fluoropyrimidine/platinum and trastuzumab has been approved as a standard treatment for HER2-positive patients. However, other agents have shown limited efficacy in first- and second-line treatments. Recent trials with HER2-directed treatment and immune checkpoint inhibitors have shown promising results. The management of HER2-positive gastric cancer requires further optimization to achieve precision medicine with a chemotherapeutic backbone.
JOURNAL OF GASTRIC CANCER
(2023)
Article
Oncology
Andrew H. Ko, Kyu-Pyo Kim, Jens T. Siveke, Charles D. Lopez, Jill Lacy, Eileen M. O'Reilly, Teresa Macarulla, Gulam A. Manji, Jeeyun Lee, Jaffer Ajani, Maria Alsina Maqueda, Sun-Young Rha, Janet Lau, Nedal Al-Sakaff, Simon Allen, Danny Lu, Colby S. Shemesh, Xinxin Gan, Edward Cha, Do-Youn Oh
Summary: This clinical trial evaluated the clinical activity of atezolizumab in combination with PEGPH20 in patients with pancreatic ductal adenocarcinoma or gastric cancer. The results showed limited clinical activity in patients with pancreatic ductal adenocarcinoma and none in patients with gastric cancer. The safety of the combination therapy was consistent with the known safety profiles of the individual agents.
Article
Oncology
Chang Gon Kim, Minkyu Jung, Hyo Song Kim, Choong-kun Lee, Hei-Cheul Jeung, Dong-Hoe Koo, Woo Kyun Bae, Dae Young Zang, Bum Jun Kim, Hyunki Kim, Un-Jung Yun, Jingmin Che, Sejung Park, Tae Soo Kim, Woo Sun Kwon, Juin Park, Sang Woo Cho, Chung Mo Nam, Hyun Cheol Chung, Sun Young Rha
Summary: Trastuzumab combined with ramucirumab and paclitaxel showed appreciable efficacy with manageable safety profiles in patients with previously treated HER2-positive G/GEJ cancer.
JOURNAL OF CLINICAL ONCOLOGY
(2023)
Article
Oncology
Funda Meric-Bernstam, Emiliano Calvo, Keun Seok Lee, Victor Moreno, Yeon Hee Park, Sun Young Rha, Pavani Chalasani, Wei Zhong, Li Zhou, Steven Pirie-Shepherd, Abraham C. F. Leung, Giuseppe Curigliano
Summary: This study evaluated the safety and antitumor activity of PF-06804103 in patients with advanced/unresectable or metastatic breast and gastric cancers. The results showed that PF-06804103 demonstrated antitumor activity, but adverse events led to a high rate of treatment discontinuation.
MOLECULAR CANCER THERAPEUTICS
(2023)
Article
Health Care Sciences & Services
Si Won Lee, Jung Hye Kwon, Seung-hoon Beom, Sang Joon Shin, Hyo Song Kim, Sun Young Rha, Minkyu Jung, Joo Hyuk Sohn, Joong-Bae Ahn, Hyun Cheol Chung, Gun Min Kim, Hye Ryun Kim, Beodeul Kang, Youn Jung Hu, Hye Jin Choi
Summary: This study evaluated the first-year outcomes of patients admitted to an acute palliative care unit (APCU) at a tertiary hospital in Korea. The results showed that during their stay in the APCU, the overall symptoms of inpatients were reduced.
PALLIATIVE MEDICINE REPORTS
(2023)
Article
Oncology
Moonki Hong, Mingee Choi, JiHyun Lee, Kyoo Hyun Kim, Hyunwook Kim, Choong-Kun Lee, Hyo Song Kim, Sun Young Rha, Gyu Young Pih, Yoon Jin Choi, Da Hyun Jung, Jun Chul Park, Sung Kwan Shin, Sang Kil Lee, Yong Chan Lee, Minah Cho, Yoo Min Kim, Hyoung-Il Kim, Jae-Ho Cheong, Woo Jin Hyung, Jaeyong Shin, Minkyu Jung
Summary: The COVID-19 pandemic has led to a significant decrease in the overall number of gastric cancer diagnoses, with cases being diagnosed at more advanced stages, especially among men and patients aged 40 and older in 2021. Further studies are needed to explore the impact of the pandemic on global gastric cancer detection.
JOURNAL OF GASTRIC CANCER
(2023)
Article
Oncology
Andrew H. Ko, Kyu-Pyo Kim, Jens T. Siveke, Charles D. Lopez, Jill Lacy, Eileen M. O'Reilly, Teresa Macarulla, Gulam A. Manji, Jeeyun Lee, Jaffer Ajani, Maria Alsina Maqueda, Sun-Young Rha, Janet Lau, Nedal Al-Sakaff, Simon Allen, Danny Lu, Colby S. Shemesh, Xinxin Gan, Edward Cha, Do-Youn Oh
Summary: This study evaluated the efficacy and safety of the combination of atezolizumab and PEGylated recombinant human hyaluronidase in patients with advanced pancreatic ductal adenocarcinoma and gastric cancer. The results showed limited clinical activity of atezolizumab plus PEGPH20 in patients with pancreatic cancer and no significant effect in patients with gastric cancer. The safety profile of this combination was consistent with the known safety profiles of the individual agents.