Journal
ONCOLOGY REPORTS
Volume 30, Issue 3, Pages 1239-1248Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2013.2596
Keywords
microRNA-128; glioma; cell-cell adhesion; erythropoietin-producing hepatocellular receptor B2
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Funding
- National Natural Science Foundation of China [81272773, 81101960]
- Scientific and Technological Planning of Guangzhou [2012J4100082]
- Fundamental Research Funds for the Central Universities [10lgpy23]
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MicroRNAs (miRNAs) are small, non-coding RNAs which regulate gene expression at the post-transcriptional level. Abnormal expression of miRNAs occurs frequently in human tumors. Despite the fact that reduced expression of miR-128 has been observed in glioma tissues and cells, the role of miR-128 in tumors has not been fully characterized. In the present study, cell adhesion assays indicated that overexpression of miR-128 can promote cell-cell adhesion. Target site prediction algorithms indicated that miR-128 binds the 3'-untranslated regions of erythropoietin-producing hepatocellular receptor (Eph)B1 and EphB2 mRNAs. Luciferase reporter assays confirmed that miR-128 binds and regulates EphB1 and EphB2 mRNAs. Overexpression of EphB2 reduced the ability of miR-128 to promote cell-cell adhesion. The wound-healing assay indicated that miR-128 significantly inhibited cell migration via EphB2. This study revealed the novel functions of miR-128 in cell-cell adhesion and cell migration in glioma cells through the regulation of EphB2, and identified EphB1 and EphB2 as novel miR-128 targets.
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