Journal
ONCOLOGY REPORTS
Volume 31, Issue 3, Pages 1466-1472Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2013.2949
Keywords
circulating cell-free DNA; preoperative chemoradiotherapy; rectal cancer
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Funding
- Young Scientist Foundation from Shanghai Municipal Health Bureau [157]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars from China State Education Ministry [N130204]
- National Natural Science Foundation of China [81202148]
- Foundation of Fudan University 985 Project [985IIIYPT06]
- Young Scientist Foundation of Fudan University Shanghai Cancer Center
- Shanghai Pujiang Program [13PJ1401600]
- Foundation of Shanghai Committee of Science and Technology of China [12DZ2260100]
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In the present study, we studied the relationship between plasma cell-free DNA and the effect of preoperative chemoradiotherapy in patients with rectal cancer. The concentration, KRAS mutation and O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation status of cell-free DNA were measured by using polymerase chain reaction (PCR) analyses. The response to chemoradiotherapy was assessed using tumor regression grading (TRG) scores. The cell-free DNA concentrations in patients with rectal cancer (n=34) were significantly higher compared to healthy controls (n=10). The 400-base pair (bp) DNA concentration, 400-/100-bp DNA ratio decreased significantly after chemoradiotherapy in the good response group. The incidence of KRAS mutation decreased significantly after chemoradiotherapy in both good and poor response groups. Higher MGMT promoter methylation status at baseline DNA was associated with a better tumor response. Therefore, cell-free DNA detection may be useful in evaluating the effect of preoperative chemoradiotherapy in patients with rectal cancer.
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