Article
Chemistry, Analytical
Alan Meng, Xiaocheng Hong, Yue Zhang, Jifang Yin, Liying Sheng, Zhenjiang Li
Summary: The electrochemical aptasensor based on L-Glutamic acid and peptides is sensitive, antifouling, and effective in detecting ATP. It shows a wide linear range, low detection limit, excellent selectivity, reproducibility, and stability. The sensor also demonstrates consistent performance in different solutions, indicating its potential for clinical analysis.
MICROCHEMICAL JOURNAL
(2021)
Article
Nanoscience & Nanotechnology
Yuhui Gao, Yingying Chen, Jinhua Shang, Shanshan Yu, Shizhen He, Ran Cui, Fuan Wang
Summary: Aptsensors with high specificity have great potential for clinical diagnosis and prognosis. However, their applications in intracellular molecular imaging are limited. In this study, a robust enzyme-free autocatalysis-driven feedback DNA circuit was developed for amplified aptasensing with significantly improved sensitivity in living cells.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Xiluan Yan, Yuanlin Ji, Yipi Xiao, Xinxin Xue, Jie Liu, Su Li, Fanrong Ai, Xiangjuan Zheng
Summary: A chemiluminescence analysis method for simultaneous detection of ATP and CAP in food was developed using a label-free dual-aptasensor, which showed high selectivity and sensitivity. The proposed method was further validated by measuring the recovery of spiked ATP/CAP in different food samples.
Article
Chemistry, Analytical
Xinjia He, Xiao Zhao, Wenfang Deng, Yueming Tan, Qingji Xie
Summary: CdSe quantum dots-decorated ZnIn2S4 nanosheets exhibit high photoelectrochemical activity for sensitive ATP detection by integrating exciton energy transfer and exciton-plasmon coupling.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Nanoscience & Nanotechnology
Xiaoyan He, Nan Sun, Hui Jia, Miaomiao Hou, Zheping Tan, Xiaoquan Lu
Summary: An antifouling electrochemical biosensor based on the interpenetrating network of Y-DNA scaffold and polyaniline hydrogel was designed for ATP detection. The sensor exhibited good detection performance and antifouling effect, providing reference value for the detection of other biological small molecules.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Amir Khojastehnezhad, Faezeh Taghavi, Elnaz Yaghoobi, Mohammad Ramezani, Mona Alibolandi, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: This article discusses the application of aptamer-based biosensors in the detection of ATP. These sensors, with high specificity, rapid response, and easy construction, can be divided into optical and electrochemical sensors, and further classified into metal-based and carbon-based materials based on QDs.
Article
Chemistry, Physical
Wei-Lun Chen, Yong-Ming Dai, Bi-Sheng Huang, Guan-Hui Lai, Mei-Hui Tsai
Summary: In this study, a novel carbon nanocomposite paste electrode modified with tetra aniline/ graphene oxide/gold nanoparticle (TA/GO/AuNP) composites was prepared for the electrochemical sensing of ascorbic acid (AA). The addition of AuNPs to the ternary composites increased the current response and detection sensitivity, indicating enhanced direct electron transfer between AA and the electrode. The results confirm that the addition of nanometer-scale components significantly affects the electrocatalytic activity.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2021)
Article
Nanoscience & Nanotechnology
Hussen Maseed, Veera Manohara Reddy Yenugu, Sesha SuryaBindu Devarakonda, Shaikshavali Petnikota, Madhavi Gajulapalli, Vadali V. S. S. Srikanth
Summary: A nano-Fe3O4/few-layered graphene (FLG) composite was prepared and used as a sensor for ultrasensitive detection of dopamine (DA). The sensor demonstrated excellent sensitivity and selectivity, and could reliably detect DA even in the presence of interfering substances uric acid (UA) and ascorbic acid (AA).
ACS APPLIED NANO MATERIALS
(2023)
Review
Chemistry, Analytical
Hossein Khosropour, Pramod K. Kalambate, Rupali P. Kalambate, Khageephun Permpoka, Xiaohong Zhou, George Y. Chen, Wanida Laiwattanapaisal
Summary: There has been a rise in pesticide use as a result of agricultural industrialization, leading to health and environmental hazards. Therefore, rapid and reliable detection of harmful organophosphorus toxins is of utmost importance. Aptasensors, as a new type of biosensor, exhibit exceptional recognition capability and flexibility. Recent research has shown that nanotechnology can enhance the sensitivity and accuracy of aptasensors.
Article
Nanoscience & Nanotechnology
Xiaoyan He, Jiandi Dong, Huimin Han, Nan Sun, Wenyu Shi, Xiong Lu, Hui Jia, Xiaoquan Lu
Summary: The newly designed electrochemical aptasensor utilizing DNA-templated copolymers is capable of sensitive detection of ATP, with a wide linear response range and high selectivity, achieving a detection limit as low as 11.5 fM.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Review
Chemistry, Multidisciplinary
Wanqing Zhang, Xijiao Li, Xiaoman Ding, Kang Hua, Aili Sun, Xinxin Hu, Ziwei Nie, Yongsheng Zhang, Jichao Wang, Renlong Li, Shanqin Liu
Summary: Metal-organic framework composites have the potential to be excellent electrode materials for electrochemical sensors due to their large surface area, high porosity, strong catalytic efficiency, and good stability. However, they still face various challenges and limitations. This review focuses on the application of MOF composites in electrochemical sensors, including MOF/carbon, MOF/metal nanoparticle, MOF/metal oxide, and MOF/enzyme composites. The challenges in using MOF composites are discussed, and the potential for future development is considered.
Article
Chemistry, Analytical
Han-Wen Zhang, Qian-Qian Zhu, Rongrong Yuan, Hongming He
Summary: The UiO-66-NH2@COF composite, constructed through a covalent linking approach, significantly enhances the electrochemical detection performance, showing promising applications in the field.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Jingjing Jiang, Dong Ding, Jing Wang, Xinyi Lin, Guowang Diao
Summary: Three-dimensional nitrogen-doped graphene networks were used as building blocks to construct a metal-free quadruplet electrochemical sensor for simultaneous detection of AA, DA, UA, and AP. The 3D-NG materials with a porous structure and nitrogen doping effect had improved electrocatalytic activities, allowing for effective differentiation of the four analytes.
Article
Chemistry, Analytical
Rui Han, Yang Li, Min Chen, Wanting Li, Caifeng Ding, Xiliang Luo
Summary: In this study, an antifouling electrochemical biosensor was developed for direct detection of circulating tumor cells (CTCs) in blood, without the need for purification or separation. The biosensor, integrated with a multifunctional peptide and conducting polymer PEDOT, exhibited enhanced sensitivity and retained linear response even in the presence of biofouling in real blood samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Shida Cao, Hongli Zhao, Kaicha Chen, Fangfang Zhou, Minbo Lan
Summary: In this study, a highly sensitive electrochemical aptasensor for adenosine detection was proposed using multi-walled carbon nanotubes (MWCNTs) loaded with PtCu nanoparticles (PtCu-MWCNTs) to amplify the signal. The PtCu-MWCNTs complex exhibited superior electrocatalytic activity towards H2O2, enabling the precise and sensitive detection of adenosine. The aptasensor showed a wide linear range and low detection limit, making it a potential candidate for point-of-care detection of adenosine.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Erhu Xiong, Xiaohua Zhang, Yunqing Liu, Jiawan Zhou, Peng Yu, Jinhua Chen
ANALYTICAL METHODS
(2016)
Article
Biophysics
Peng Yu, Xiaohua Zhang, Erhu Xiong, Jiawan Zhou, Xiaoyu Li, Jinhua Chen
BIOSENSORS & BIOELECTRONICS
(2016)
Article
Biophysics
Peng Yu, Yunqing Liu, Xiaohua Zhang, Jiawan Zhou, Erhu Xiong, Xiaoyu Li, Jinhua Chen
BIOSENSORS & BIOELECTRONICS
(2016)
Article
Chemistry, Analytical
Erhu Xiong, Xiaoxia Yan, Xiaohua Zhang, Yanmei Li, Ruiying Yang, Leixia Meng, Jinhua Chen
Article
Chemistry, Analytical
Erhu Xiong, Deshuai Zhen, Ling Jiang, Xiaoming Zhou
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Multidisciplinary
Menglu Hu, Chaoqun Yuan, Tian Tian, Xusheng Wang, Jian Sun, Erhu Xiong, Xiaoming Zhou
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Chemistry, Multidisciplinary
Huahua Yue, Mengqi Huang, Tian Tian, Erhu Xiong, Xiaoming Zhou
Summary: CRISPR-based diagnoses, derived from gene-editing technology, have unique features such as recognition at physiological temperature, excellent specificity, and high-efficiency signal amplification capabilities. Micro/nanotechnologies play key roles in the advancement of CRISPR diagnosis, including the use of nanoprobes, nanochips, microfluidic technology, and lateral flow strips, leading to new opportunities and improvements such as equipment-free detection, more compact detection systems, improved sensitivity, and quantitative capabilities. Despite significant progress, CRISPR diagnosis has not yet reached its full potential, with upcoming opportunities and improvements expected to be driven by micro/nanotechnologies.
Article
Chemistry, Multidisciplinary
Huahua Yue, Bowen Shu, Tian Tian, Erhu Xiong, Mengqi Huang, Debin Zhu, Jian Sun, Qing Liu, Shichan Wang, Yirong Li, Xiaoming Zhou
Summary: This study presents a CRISPR-Cas12a-based molecular diagnostic technique for amplification-free and absolute quantification of DNA at the single-molecule level, achieving high sensitivity and specificity through optimized reaction parameters and microdroplet technology. The method can directly count diverse viruses' DNAs and demonstrate versatility and quantification capability, showing potential as a versatile and quantitative platform for molecular diagnosis.
Article
Chemistry, Analytical
Mei Lin, Huahua Yue, Tian Tian, Erhu Xiong, Debin Zhu, Yongzhong Jiang, Xiaoming Zhou
Summary: A glycerol-enhanced one-pot RPA-CRISPR/Cas12a method has been discovered to greatly improve the detection efficiency, making it comparable to the conventional two-step method. The enhanced efficiency is attributed to the phase separation of the RPA and CRISPR/Cas12a system caused by glycerol viscosity.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Ling Jiang, Jinlian Du, Haili Xu, Xiaohua Zhuo, Jinlong Ai, Jiayu Zeng, Ronghua Yang, Erhu Xiong
Summary: In this study, a novel CRISPR/Cas13a-mediated photoelectrochemical biosensor was proposed for the direct assay of miRNA-21, which showed improved specificity and sensitivity compared to traditional nucleic acid-based strategies. The CRISPR/Cas13a system accurately recognized and efficiently cleaved the target, without the need for complex nucleic acid sequence design. This biosensor demonstrated excellent analysis capability and stability, suggesting promising application prospects in early disease diagnosis and biomedical research.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Erhu Xiong, Ling Jiang
Article
Chemistry, Analytical
Erhu Xiong, Zhenzhen Li, Xiaohua Zhang, Jiawan Zhou, Xiaoxia Yan, Yunqing Liu, Jinhua Chen
ANALYTICAL CHEMISTRY
(2017)
Article
Biophysics
Erhu Xiong, Xiaoxia Yan, Xiaohua Zhang, Yunqing Liu, Jiawan Zhou, Jinhua Chen
BIOSENSORS & BIOELECTRONICS
(2017)
Article
Biophysics
Yunqing Liu, Erhu Xiong, Xiaoyu Li, Junjing Li, Xiaohua Zhang, Jinhua Chen
BIOSENSORS & BIOELECTRONICS
(2017)
Article
Chemistry, Multidisciplinary
Erhu Xiong, Deshuai Zhen, Ling Jiang
CHEMICAL COMMUNICATIONS
(2018)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).
