Article
Food Science & Technology
Yuan Wang, Lijie Xing, Jinlei Zhang, Xiaoning Ma, Rui Weng
Summary: To achieve comprehensive analysis of phenolic compounds in honey, a simple and reliable method using HPLC-MS/MS was developed. 24 compounds were simultaneously analyzed with good linearity and high sensitivity. The method proved to be suitable for the analysis of phenolic compounds in honey samples.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemical Research Methods
Andrea Gottardini, Vincent Dugas, Claire Demesmay
Summary: Affinity chromatography is a powerful technique for identifying and quantifying weak ligand-protein interactions. A novel approach of protein immobilization based on streptavidin-biotin interaction was presented in this study to improve bulk density of active protein, expanding the affinity range of the technique to the millimolar range.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Multidisciplinary
Worathat Thitikornpong, Ponsiree Jithavech, Somphob Thompho, Yanyong Punpreuk, Hasseri Halim, Boonchoo Sritularak, Pornchai Rojsitthisak
Summary: A simple UPLC-UV method for simultaneous determination of bioactive polyphenols in orchid plants has been developed, providing an effective tool for evaluating the distribution of phytochemicals in plants. The method is simple, accurate, and suitable for routine quality control analysis.
ARABIAN JOURNAL OF CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Michal Kaspar, Tomas Bajer, Petra Bajerova, Petr Cesla
Summary: In this study, a method combining gas chromatography and high-performance liquid chromatography was developed to comprehensively analyze the content of phenolic compounds in different types of Balsamic vinegar. The major phenolic compounds identified in Balsamic vinegar were gallic acid, protocatechuic acid, caffeic acid, and p-coumaric acid.
Article
Plant Sciences
Rima Sedbare, Sigita Sprainaityte, Gintaras Baublys, Jonas Viskelis, Valdimaras Janulis
Summary: The fruits of Vaccinium oxycoccos L. are rich in bioactive compounds with antibacterial, antifungal, and anti-inflammatory effects. Phytochemical analysis of cranberry fruit samples collected from different wetland sites showed variations in the qualitative and quantitative composition of these compounds. These findings emphasize the importance of standardizing the composition of cranberry fruit raw materials and conducting routine tests to expand their use in functional foods and phytopreparations production.
Review
Chemistry, Multidisciplinary
Michal Kaspar, Petr Cesla
Summary: This article reviews and discusses the production and analysis methods of vinegars, with a focus on the unique production process of balsamic vinegar in Modena and the analysis methods for its chemical composition.
APPLIED SCIENCES-BASEL
(2022)
Article
Chemistry, Applied
Victoria M. de Campos, Mariane A. Andrade, Edvaldo V. S. Maciel, Ana Lucia de Toffoli, Fernando M. Lancas
Summary: Increased use of environmentally friendly practices is a trend in science due to the current awareness of climate change. This study proposes a method for pre-concentrating and cleaning up sulphonamide drugs in honey using a miniaturised extraction column. Several variables were optimised, and the method showed good linearity, selectivity, and recovery.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2022)
Article
Biochemistry & Molecular Biology
Linhui Zhou, Xinyu Duan, Kunning Li, David R. A. Hill, Gregory J. O. Martin, Hafiz A. R. Suleria
Summary: This study compared and analyzed the free and bound phenolic compounds in different marine microalgae species. The results showed significant differences in total phenolic content, total flavonoid content, and total condensed tannin content among the species, highlighting unique phenolic profiles. A total of ten phenolic compounds were identified, including previously unreported compounds in microalgae samples. These findings have important implications for future industrial applications.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Chemistry, Analytical
Szabolcs Fekete, Mathew DeLano, Andrew Bates Harrison, Stephen J. Shiner, Jonathan Leon Belanger, Kevin D. Wyndham, Matthew A. Lauber
Summary: In order to address the challenges in analyzing certain biomolecules using liquid chromatography, researchers have developed a new hydrophilically modified hybrid surface (h-HST) for size exclusion chromatography and anion exchange separations. By incorporating additional oxygen and carbon atoms onto an ethylene bridge hybrid siloxane polymer, this surface reduces non-specific adsorption and undesired secondary interactions, resulting in improved separations of proteins and nucleic acids.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
E. Gonzalez-Garcia, M. L. Marina, M. C. Garcia
Summary: A green methodology based on pressurized liquids (PLE) was proposed for extracting proteins and obtaining highly active extracts from brewer's spent grain (BSG). Compared to conventional alkaline extraction assisted with ultrasounds (UAE), PLE extracted more proteins and showed higher antioxidant, cholesterol esterase inhibition, and ACE inhibitory activities. HPLC-MS/MS analysis revealed that PLE extracts had a higher ratio of hydrophobic peptides, which contributed to their higher bioactivity.
Review
Chemistry, Analytical
Hanne Roberg-Larsen, Steven Ray Wilson, Elsa Lundanes
Summary: This review focuses on the application of nLC-MS in sample preparation, particularly through online upfront devices and online reaction systems to improve the sensitivity and robustness of samples.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Deyber Arley Vargas Medina, Natalia Gabrielly Pereira dos Santos, Edvaldo Vasconcelos Soares Maciel, Fernando Mauro Lancas
Summary: This article reviews the development of nanoLC technology and its integration with EI-MS, exploring new advances and discussing limitations in the field. It provides the reader with a current overview of nanoLC technology and highlights future research directions.
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
(2021)
Article
Chemistry, Multidisciplinary
Nina I. Kashchenko, Daniil N. Olennikov, Nadezhda K. Chirikova
Summary: In this study, S. repens was selected for chemical analysis, revealing the presence of ecdysteroids and glycosylflavones in its seedlings. The impact of exogenous phytohormones and elicitors on compound productivity and accumulation was investigated for the first time in S. repens seedlings. Antioxidant activity against DPPH radical was determined, with specific compounds showing high activity.
APPLIED SCIENCES-BASEL
(2021)
Article
Agriculture, Multidisciplinary
Raul Gonzalez-Dominguez, Ana Sayago, Maria Santos-Martin, Angeles Fernandez-Recamales
Summary: This study describes the optimization and validation of a high-throughput and robust metabolomics method based on liquid chromatography for the identification and quantification of phenolic compounds and related metabolites. The method was successfully validated and applied to characterize phenolic fingerprints in food and biological samples.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Maria Marhuenda-Munoz, Ines Dominguez-Lopez, Emily P. Laveriano-Santos, Isabella Parilli-Moser, Cristina Razquin, Miguel Ruiz-Canela, Francisco Javier Basterra-Gortari, Dolores Corella, Jordi Salas-Salvado, Montserrat Fito, Jose Lapetra, Fernando Aros, Miquel Fiol, Lluis Serra-Majem, Xavier Pinto, Enrique Gomez-Gracia, Emilio Ros, Ramon Estruch, Rosa M. Lamuela-Raventos
Summary: The study found that changes in urinary microbial phenolic metabolites were associated with the risk of developing type 2 diabetes. However, when adjusting for fasting plasma glucose, this association lost statistical significance. Changes in dietary patterns may affect the production of metabolites and alter the gut microbial population.
Review
Food Science & Technology
Javad Sharifi-Rad, Charles Oluwaeun Adetunji, Olugbemi T. Olaniyan, Stephen Kayode Ojo, Micheal Olugbenga Samuel, Banjo Temitope Temitayo, Osahon Itohan Roli, Olaogun Oluwatoyin Nimota, Banjo Temitope Oluwabunmi, Juliana Bunmi Adetunji, Farukh Sharopov, Natalia Cruz-Martins, Maria del Mar Contreras
Summary: This article reviews the antimicrobial and antioxidant activities of Ocimum plants, their use in food preservation, the phytochemical composition, and other pharmacological properties. It concludes that while these plants have potential as food preservatives and functional ingredients, there are still challenges to be addressed.
FOOD REVIEWS INTERNATIONAL
(2023)
Review
Biochemistry & Molecular Biology
Alvaro Fernandez-Ochoa, Maria de la Luz Cadiz-Gurrea, Patricia Fernandez-Moreno, Alejandro Rojas-Garcia, David Arraez-Roman, Antonio Segura-Carretero
Summary: The study of bioavailability of bioactive compounds is crucial for the development of applications. Recent advances in analytical chemistry and metabolomics have contributed to the understanding of the metabolism and bioavailability of phenolic and terpenoid compounds. This review highlights the role of analytical chemistry and metabolomics in this field, detailing the different steps of the analytical chemistry workflow and presenting the most relevant results obtained.
Review
Cell Biology
Monica Butnariu, Cristina Quispe, Jesus Herrera-Bravo, Marius Pentea, Ioan Sarac, Aylin Seylam Kusumler, Beraat Ozcelik, Sakshi Painuli, Prabhakar Semwal, Muhammad Imran, Tanweer Aslam Gondal, Simin Emamzadeh-Yazdi, Natallia Lapava, Zubaida Yousaf, Manoj Kumar, Ali Hussein Eid, Yusra Al-Dhaheri, Hafiz Ansar Rasul Suleria, Maria del Mar Contreras, Javad Sharifi-Rad, William C. Cho
Summary: This review focuses on the phytochemical composition and pharmacological activities of Papaver plants, including alkaloids, phenolic compounds, and essential oils. Papaver somniferum, in particular, is known for supplying important alkaloids such as morphine. The plants also exhibit various pharmacological properties, including analgesic, anticancer, antimicrobial, antioxidant, and antidiabetic activities.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Review
Biochemistry & Molecular Biology
Abigail Garcia-Villegas, Alejandro Rojas-Garcia, Maria del Carmen Villegas-Aguilar, Patricia Fernandez-Moreno, Alvaro Fernandez-Ochoa, Maria de la Luz Cadiz-Gurrea, David Arraez-Roman, Antonio Segura-Carretero
Summary: The review discusses the bioactive compounds found in tropical fruit by-products and their potential therapeutic properties for skin health in the cosmeceutical industry. Various extraction techniques are also explored, along with the effects of these compounds on oxidative stress, hyperpigmentation, acne, aging, and UV radiation.
Review
Nutrition & Dietetics
Alejandro Rojas-Garcia, Alvaro Fernandez-Ochoa, Maria de la Luz Cadiz-Gurrea, David Arraez-Roman, Antonio Segura-Carretero
Summary: Neurodegenerative diseases are characterized by cell damage, impaired nervous system connections, and neuronal death, leading to the loss of essential motor and cognitive functions. Bioactive compounds, particularly phenolic compounds found in plant by-products, have shown promising neuroprotective effects and could be an optimal strategy for valorizing agricultural residues. This review summarizes the triggers of neurodegeneration, examines the role of plant extracts in neuroprotection, and discusses the potential neuroprotective effects of phenolic compounds.
Article
Biochemistry & Molecular Biology
Gokhan Zengin, Alvaro Fernandez-Ochoa, Maria de la Luz Cadiz-Gurrea, Francisco Javier Leyva-Jimenez, Antonio Segura-Carretero, Fevzi Elbasan, Evren Yildiztugay, Sumira Malik, Asaad Khalid, Ashraf N. Abdalla, Mohamad Fawzi Mahomoodally
Summary: This study investigated the phytochemical profile and biological properties of Paliurus spina-christi Mill., a medicinal plant traditionally used for various human ailments. The methanol extract exhibited the strongest antioxidant and anti-tyrosinase activities. These findings contribute to the understanding of P. spina-christi as a potential source of naturally occurring bioactive compounds with biomedical applications.
Article
Biology
Afoua Mufti, Maria del Mar Contreras, Irene Gomez-Cruz, Abdullah Alshamrani, Saber Nahdi, Lamjed Mansour, Salah Alwasel, Abdel Halim Harrath, Nizar Tlili
Summary: The aim of this study was to analyze the phytochemical content of Ephedra alata pulp extract (EAP) and investigate its antioxidant and anti-inflammatory capacities. High-performance liquid chromatography-electrospray ionization-quadrupole-time-of-flight mass spectrometry (HPLC-ESI-QTOF/MS) was used for phytochemical analysis, and various in vitro antioxidant and anti-inflammatory tests were conducted. The results showed that EAP contains 42 metabolites, including flavonoids, sphingolipids, fatty acids, ephedrine derivatives, and amino acid derivatives. EAP exhibited strong antioxidant activity, as well as notable anti-inflammatory effects by inhibiting COX-1 and COX-2, preventing protein denaturation, and protecting membrane stabilization.
Article
Biochemistry & Molecular Biology
Elixabet Diaz-de-Cerio, Francisco Giron, Alfonso Perez-Garrido, Andreia S. P. Pereira, Jose Antonio Gabaldon-Hernandez, Vito Verardo, Antonio Segura Carretero, Horacio Perez-Sanchez
Summary: This study identified 73 phenolic compounds from Spanish guava leaves and found that some of these compounds interact with diabetes-related proteins, suggesting their potential anti-diabetic activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jose Alberto Molina-Tijeras, Antonio Jesus Ruiz-Malagon, Laura Hidalgo-Garcia, Patricia Diez-Echave, Maria Jesus Rodriguez-Sojo, Maria de la Luz Cadiz-Gurrea, Antonio Segura-Carretero, Jose Perez del Palacio, Maria Reyes Gonzalez-Tejero, Maria Elena Rodriguez-Cabezas, Julio Galvez, Alba Rodriguez-Nogales, Teresa Vezza, Francesca Algieri
Summary: Obesity, a global public health problem, is increasing in prevalence rate. Plant-based treatments, such as Lavandula multifida extract (LME), have been gaining attention as a means to manage obesity and its comorbidities. In this study, LME was found to reduce weight gain, improve insulin sensitivity and glucose tolerance, decrease inflammation in the liver and adipose tissue, regulate gut permeability, and reduce oxidative stress. These findings suggest that LME may be a promising supplementary approach for managing obesity and its comorbidities.
Editorial Material
Nutrition & Dietetics
Carmen del Rio, Antonio Segura-Carretero
Article
Biochemistry & Molecular Biology
Abigail Garcia-Villegas, Alvaro Fernandez-Ochoa, Alejandro Rojas-Garcia, Maria Elena Alanon, David Arraez-Roman, Maria de la Luz Cadiz-Gurrea, Antonio Segura-Carretero
Summary: This study evaluates the bioactive potential of mango peel extract and demonstrates its promising use as an ingredient in cosmetic formulations.
Review
Chemistry, Physical
Deborah Perez-Almada, Angel Galan-Martin, Maria del Mar Contreras, Eulogio Castro
Summary: Biorefineries play a crucial role in achieving circular economy and sustainability goals. This comprehensive review of the Environmental and Techno-Economic Assessment (ETEA) of biorefineries provides insights into the technical, economic, and environmental implications of different biorefinery schemes. The study reveals increasing interest in the topic, with a focus on second-generation biomass and biochemical routes such as fermentation and anaerobic digestion. The ETEA framework contributes to the optimization of biorefineries and trade-off evaluation between economic viability and environmental sustainability.
SUSTAINABLE ENERGY & FUELS
(2023)
Article
Food Science & Technology
Maria del Mar Contreras, Anouar Feriani, Irene Gomez-Cruz, Najla Hfaiedh, Abdel Halim Harrath, Inmaculada Romero, Eulogio Castro, Nizar Tlili
Summary: A grapevine shoot extract (GSE) containing stilbenoids, particularly trans-resveratrol and trans-epsilon-viniferin, was obtained using ultrasound-assisted extraction. GSE administration to a myocardial injury animal model showed improved plasma lipid profile, reduced plasma ion concentration disturbance, cardiac dysfunction, DNA damage, and necrosis of myocardial tissue. These effects are likely due to the antioxidant properties of GSE, increased levels of endogenous antioxidants, and decreased lipid peroxidation markers in the heart. The study also demonstrated ACE-inhibitory activity, indicating the potential of GSE in cardiovascular disease prevention. Our findings suggest that GSE, containing trans-resveratrol and its derivatives, has the potential to be used in the development of innovative functional ingredients.
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).