Article
Biochemistry & Molecular Biology
Hala A. Hafez, Ali M. Atoom, Rana H. M. Khafaga, Sara A. Shaker, Maher A. Kamel, Nagwa M. Assem, Shimaa A. Mahmoud
Summary: The study found that the therapeutic dose of SOF has adverse effects on mitochondrial biogenesis and function in different tissues of young female rats, with the most significant effects observed in ovarian tissues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Takuto Kumano, Sanae Hori, Satomi Watanabe, Yuzu Terashita, Hong Yang Yu, Yoshiteru Hashimoto, Toshiya Senda, Miki Senda, Michihiko Kobayashi
Summary: The study identified an enzyme named CarA that plays a crucial role in carminic acid metabolism by oxidizing the sugar moiety. Homologs of CarA were found to be widely distributed in soil microorganisms and important for C-glycoside metabolism.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Pharmacology & Pharmacy
Pengfei He, Sha Wang, Sen Li, Siqi Liu, Shuqi Zhou, Jing Wang, Jiayue Tao, Dongdong Wang, Rufeng Wang, Wenfu Ma
Summary: Recently, scientists have discovered an enzyme called DgpA/B/C from a human intestinal bacterium that can specifically cleave the C-glycosidic bond of puerarin and also has the activity as a structure isomerase. Through crystal structure and biochemical characterization, they have revealed how DgpA recognizes and oxidizes puerarin and proposed a possible mechanistic model. These findings not only contribute to the understanding of enzyme-mediated C-glycosidic bond breakage and formation, but also have the potential to facilitate stereospecific C-glycoside synthesis in the pharmaceutical industry.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Chemistry, Physical
Qiu-Qi Mu, Ao-Xin Guo, Xin Cai, Yang-Yang Qin, Xin-Yu Fang, Li-Cong Peng, Chao Feng, Li-Wen Xu, Xiong Xiao, Xue-Wei Liu
Summary: This article reports a new C-glycoside anomerization reaction that enables the conversion of α- to β- configuration. The researchers demonstrated through experiments that this reaction likely proceeds through activation of the C(sp3)-H bond mediated by the catalyst and reversible hydrogen transfer, rather than traditional anomerization mechanisms.
Article
Chemistry, Physical
Qiu-Qi Mu, Ao-Xin Guo, Xin Cai, Yang-Yang Qin, Xin-Yu Fang, Li-Cong Peng, Chao Feng, Li-Wen Xu, Xiong Xiao, Xue-Wei Liu
Summary: Through the study of catalytic C-glycosylation reactions, we discovered the possibility of 2-deoxy-C-glycoside anomerization and explored the mechanistic details.
Article
Biochemistry & Molecular Biology
Xiang Zhou, Liming Wang, Fuyi Zhang, Yufen Zhao
Summary: Heteroaryl diketoalkynyl C-glycoside and dialkynyl di-C-glycoside analogues were synthesized through successive coupling of heteroaromatics, oxalyl chloride and terminal sugar alkynes in one pot. The three-component coupling reaction catalyzed by CuI gave heteroaryl diketoalkynyl C-glycosides. The same three-component coupling in the presence of n-BuLi produced dialkynyl di-C-glycosides, and the corresponding esters of dialkynyl di-C-glycosides were obtained with a 1:1 molar ratio of heteroaromatics to terminal sugar alkynes. The method showed good yields and was suitable for different heteroaromatics and terminal sugar alkynes.
CARBOHYDRATE RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Masamitsu Yamaguchi, Sue Cotterill
Summary: Replicative DNA polymerases are linked to human diseases, and Drosophila is a useful model for studying this relationship.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Liangqin Xie, Zeyuan Deng, Jie Zhang, Huanhuan Dong, Wei Wang, Banghuai Xing, Xiaoru Liu
Summary: This study compared the differences between flavonoid O-glycoside and C-glycoside in terms of their antioxidant activities and metabolism. The aglycones had higher activity but unstable structures, while the sugar substituents reduced the activity and improved stability. Moreover, C-glycoside was more stable than O-glycoside, which contributed to their differences in absorption and metabolism in vivo.
Article
Multidisciplinary Sciences
Nicholas Delihas
Summary: This study reports a new and different mechanism of de novo gene birth based on the discovery and characteristics of a spacer sequence. The spacer sequence acts as a starting site for DNA synthesis and leads to the formation of different genes by adding other sequences.
Article
Chemistry, Multidisciplinary
Naresh Bhuma, Ludivine Lebedel, Hiroki Yamashita, Yutaka Shimizu, Zahra Abada, Ana Arda, Jerome Desire, Bastien Michelet, Agnes Martin-Mingot, Ali Abou-Hassan, Masahiro Takumi, Jerome Marrot, Jesus Jimenez-Barbero, Aiichiro Nagaki, Yves Bleriot, Sebastien Thibaudeau
Summary: Ferrier's research in 1962 on the transformation of glycals into unsaturated glycosyl derivatives involved an alpha,beta unsaturated glycosyl cation. Through NMR-based structural analysis and DFT calculations, this elusive ionic species was characterized, leading to the synthesis of unsaturated nitrogen-containing C-aryl glycosides and C-alkyl glycosides under different conditions.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Qikai Sun, Huixing Zhang, Quanquan Wang, Tianjiao Qiao, Gang He, Gong Chen
Summary: A new strategy has been developed for the stereoselective synthesis of C-vinyl glycosides, using Pd-catalyzed directed C-H glycosylation. This method offers a broadly applicable approach to streamline the synthesis of complex C-vinyl glycosides with high efficiency and excellent regio- and stereoselectivity.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Huimin Wang, Jiaoli Wang, Ruiting Liu, Zhenxiang Liu, Kemin Wang, Jin Huang
Summary: This article introduces a new isothermal nucleic acid amplification technique, which can achieve miRNA amplification detection through reasonable probe design, avoiding the increase in cost and operation steps caused by temperature changes or the addition of additional reagents.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Chen-Hsu Yu, Adam M. Kabza, Jonathan T. Sczepanski
Summary: L-oligonucleotides, known for their intrinsic nuclease resistance, are increasingly used in the development of molecular tools and sensors. Researchers have introduced a straightforward and versatile method to assemble long L-RNAs using T4 RNA ligase 1, demonstrating compatibility with different functional classes of L-RNA.
CHEMICAL COMMUNICATIONS
(2021)
Article
Oncology
Sharlene Dong, Heba Zakaria, David Hsiehchen
Summary: Inactivating mutations in the exonuclease domain of POLE induce a high tumor mutation burden and are associated with immune checkpoint inhibitor benefit. This study shows that missense non-exonuclease domain POLE mutations are also associated with better overall survival in patients with low or intermediate tumor mutation burden treated with ICIs.
Article
Multidisciplinary Sciences
Raquel Luzon-Hidalgo, Valeria A. Risso, Asuncion Delgado, Eduardo Andres-Leon, Beatriz Ibarra-Molero, Jose M. Sanchez-Ruiz
Summary: Research has shown that viruses can adapt to different environments by mutations in viral RNA polymerase, which may lead to promiscuous recruitment of thioredoxin. This mechanism may play a role in cross-species transmission, and phenotypic mutations generally contribute to the capability of viruses to evade antiviral strategies.