New insights into the QuikChangeTM process guide the use of Phusion DNA polymerase for site-directed mutagenesis

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

A rapid and efficient one-step site-directed deletion, insertion, and substitution mutagenesis protocol

(2012) Deguang Wu et al.   ANALYTICAL BIOCHEMISTRY

Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting

(2012) Jun Fu et al.   NATURE BIOTECHNOLOGY

An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol

(2009) Huanting Liu et al.   BMC BIOTECHNOLOGY

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

A one-step PCR-based method for rapid and efficient site-directed fragment deletion, insertion, and substitution mutagenesis

(2008) Dong Qi et al.   JOURNAL OF VIROLOGICAL METHODS

IDT SciTools: a suite for analysis and design of nucleic acid oligomers

(2008) R. Owczarzy et al.   NUCLEIC ACIDS RESEARCH

Site-directed mutagenesis by combination of homologous recombination and DpnI digestion of the plasmid template in Escherichia coli

(2007) Jing Li et al.   ANALYTICAL BIOCHEMISTRY