Article
Chemistry, Multidisciplinary
Owen Zacarias, Ana G. Petrovic, Rinat Abzalimov, Padmanava Pradhan, Elise Champeil
Summary: This study presents a synthetic route to access oligonucleotides containing monoadducts formed by MC at the adenine base. Structural evidence of the adducted oligonucleotides was provided through enzymatic digestion and high-resolution mass spectral analysis, and the stability and physical properties of these substrates were compared. Additionally, virtual models were created to explore the conformational space and structural features of these MC-DNA complexes.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Sally B. Morton, L. David Finger, Roxanne van der Sluijs, William D. Mulcrone, Michael Hodskinson, Christopher L. Millington, Christina Vanhinsbergh, Ketan J. Patel, Mark J. Dickman, Puck Knipscheer, Jane A. Grasby, David M. Williams
Summary: DNA interstrand cross-links (ICLs) have significant implications for DNA replication and transcription, and understanding their repair is crucial. This study presents an efficient method for generating reduced acetaldehyde ICLs, which can be used to study their recognition and repair. The methodology described here shows promising results and will facilitate further research in cross-link repair.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Asha Mary Joseph, Kazi Nahar, Saheli Daw, Md. Mahbub Hasan, Rebecca Lo, Tung B. K. Le, Khondaker Miraz Rahman, Anjana Badrinarayanan
Summary: Pyrrolobenzodiazepines (PBDs) are DNA binding compounds with anti-tumor and anti-bacterial activity. Chemical modifications can enhance their DNA binding and efficacy. Our study shows that PBD monomers cause DNA damage and kill bacteria, and repairing these damages rely on error-free mechanisms.
RSC MEDICINAL CHEMISTRY
(2022)
Article
Ophthalmology
Rekha Mudhol, Rolika Bansal
Summary: Both HealaFlow (R) and low-dose MMC are equally safe and efficacious in trabeculectomy, with significant IOP reduction and good bleb morphology outcomes. The success rates postoperatively were similar for both methods.
INDIAN JOURNAL OF OPHTHALMOLOGY
(2021)
Review
Genetics & Heredity
Kurt Housh, Jay S. Jha, Tuhin Haldar, Saosan Binth Md Amin, Tanhaul Islam, Amanda Wallace, Anuoluwapo Gomina, Xu Guo, Christopher Nel, Jesse W. Wyatt, Kent S. Gates
Summary: Genome integrity is crucial for life, and DNA repair systems have evolved to remove unavoidable DNA lesions. Many life forms have the ability to remove DNA interstrand cross-links (ICLs), but the identity of the naturally-occurring substances that drove the evolution and retention of these DNA repair systems is still uncertain. This review discusses various chemical processes through which endogenous ICLs may be introduced into cellular DNA, involving DNA degradation processes, reactions with low molecular weight aldehyde metabolites, and reactions with DNA radicals generated by oxidation.
Article
Chemistry, Multidisciplinary
Manuel M. Paz, Elise Champeil
Summary: This article reviews the latest research on mitomycin C (MC) and dicarbamoylmitomycin C (DMC), discussing their DNA alkylation mechanism, synthesis methods, and their role in cytotoxicity.
Article
Genetics & Heredity
Jin Tang, Feng Tang, Linlin Zhao
Summary: DNA interstrand cross-links (ICLs) are lesions formed by a covalent bond between DNA strands that prevent their separation, which is necessary for DNA interpretation. Researchers have developed a simple method for preparing model ICL unhooked intermediates derived from abasic (AP) sites.
Article
Biochemistry & Molecular Biology
Zida Zhu, Taisuke Kitano, Masami Morimatsu, Arisa Tanaka, Ryo Morioka, Xianghui Lin, Koichi Orino, Yasunaga Yoshikawa
Summary: In this study, we found that the expression of BRCA2 CTRBD protein enhances the resistance of cells to X-ray irradiation and mitomycin C by increasing homologous recombination efficiency for DNA repair. This finding provides a new approach for protecting cells against the damage caused by radiation therapy and chemotherapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Chun-Lai Zhao, Xin Qiao, Xiao-Meng Liu, Xue-Qing Song, Dan-Qing Li, Xia-Wen Yu, Wei-Guo Bao, Jing-Yuan Xu
Summary: This study demonstrates that Pt(IV) complex oxoplatin can bind to DNA in a tetravalent state and rapidly produce stable interstrand crosslinks, leading to cancer cell death. Additionally, oxoplatin can induce a quick intracellular response of the FA/BRCA pathway in cancer cells, independent of intracellular glutathione levels.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lisa Schubert, Ivo A. Hendriks, Emil P. T. Hertz, Wei Wu, Selene Selles-Baiget, Saskia Hoffmann, Keerthana Stine Viswalingam, Irene Gallina, Satyakrishna Pentakota, Bente Benedict, Joachim Johansen, Katja Apelt, Martijn S. Luijsterburg, Simon Rasmussen, Michael Lisby, Ying Liu, Michael L. Nielsen, Niels Mailand, Julien P. Duxin
Summary: This study reveals an essential role of SCAI in ensuring error-free ICL repair upon activation of the FA pathway. SCAI forms a complex with Pol zeta and localizes to ICLs during DNA replication. In the absence of SCAI, HR-mediated ICL repair is defective, resulting in deletions and radial chromosomes.
Article
Cell Biology
Marian Kupculak, Fengxiang Bai, Qiang Luo, Yasunaga Yoshikawa, David Lopez-Martinez, Hannan Xu, Stephan Uphoff, Martin A. Cohn
Summary: This study identifies 10 phosphorylation sites on FANCD2, which are phosphorylated by ATR in response to DNA interstrand crosslinks (ICLs). These phosphorylation events are critical for loading of the FANCD2/FANCI complex onto chromosomes and subsequent monoubiquitination. The regulation of these phosphorylation events is important for maintaining the proper function of FANCD2 in cells.
Article
Chemistry, Multidisciplinary
Leander Simon Runtsch, Michael Stadlmeier, Alexander Schoen, Markus Mueller, Thomas Carell
Summary: fdU and fdC are formyl-containing nucleosides created by oxidative stress in differentiated cells, with fdU showing a higher reactivity towards lysine side chains compared with fdC. While fdC is not only formed as an oxidative lesion but also commonly seen in stem cells in an epigenetically important oxidation reaction.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Kimon Lemonidis, Martin L. Rennie, Connor Arkinson, Viduth K. Chaugule, Mairi Clarke, James Streetley, Helen Walden
Summary: The monoubiquitination of the FANCI-FANCD2 (ID2) complex plays a crucial role in the repair of DNA interstrand crosslinks. This study presents the cryo-EM structure of the FANCI-ubiquitinated ID2 complex (I(Ub)D2) bound to DNA, showing that the complex adopts a closed conformation and clamps on DNA. The study also reveals that FANCD2 and FANCI have primed lysine residues ready for ubiquitination in the complex. Furthermore, FANCI ubiquitination maintains FANCD2 ubiquitination by preventing excessive deubiquitination and enabling re-ubiquitination within the I(Ub)D2 complex on DNA.
Article
Biochemistry & Molecular Biology
Sunbok Jang, Matthew A. Schaich, Cindy Khuu, Brittani L. Schnable, Chandrima Majumdar, Simon C. Watkins, Sheila S. David, Bennett Van Houten
Summary: The study reveals that UV-DDB can stimulate MUTYH turnover rate, increase complex formation between MUTYH and UV-DDB, and enhance MUTYH mobility and dissociation rate during 8-oxoG:A repair, suggesting a facilitative role of UV-DDB in MUTYH activity at abasic sites.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Ericka Marel Quezada-Maldonado, Yolanda I. Chirino, Maria Eugenia Gonsebatt, Rocio Morales-Barcenas, Yesennia Sanchez-Perez, Claudia M. Garcia-Cuellar
Summary: PM10 exposure leads to accumulation of DNA damage and inhibition of DNA repair, contributing to the development of lung cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)