Journal
NUCLEIC ACIDS RESEARCH
Volume 38, Issue 20, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkq757
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Funding
- Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan
- MEXT
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We had previously exploited a method for targeted DNA methylation in budding yeast to succeed in one-hybrid detection of methylation-dependent DNA-protein interactions. Based on this finding, we developed a yeast one-hybrid system to screen cDNA libraries for clones encoding methylated DNA-binding proteins. Concurrent use of two independent bait sequences in the same cell, or dual-bait system, effectively reduced false positive clones, which were derived from methylation-insensitive sequence-specific DNA-binding proteins. We applied the dual-bait system to screen cDNA libraries and demonstrated efficient isolation of clones for methylated DNA-binding proteins. This system would serve as a unique research tool for epigenetics.
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