Journal
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT
Volume 638, Issue 1, Pages 171-175Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.nima.2011.02.085
Keywords
X-ray Imaging; Cryogenic X-ray microscopy; Diffraction microscopy; Anti-contamination
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Funding
- Division of Materials Sciences and Engineering, Office of Basic Energy Sciences [DE-FG02-07ER46128]
- National Institutes for Health [5R21EB6134]
- U.S. Department of Energy [DE-AC02-05CH11231]
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Cryogenic microscopy allows one to view frozen hydrated biological and soft matter specimens with good structural preservation and a high degree of stability against radiation damage. We describe a liquid nitrogen-cooled anti-contamination device for cryogenic X-ray diffraction microscopy. The anti-contaminator greatly reduces the buildup of ice layers on the specimen due to condensation of residual water vapor in the experimental vacuum chamber. We show by coherent X-ray diffraction measurements that this leads to fivefold reduction of background scattering, which is important for far-field X-ray diffraction microscopy of biological specimens. Published by Elsevier B.V.
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