Article
Biochemistry & Molecular Biology
Carolina da Silva Canielles Caprara, Tatiane Ksyvickas Mathias, Maria de Fatima C. Santos, Marcelo G. M. D'Oca, Caroline Da R. M. D'Oca, Fabio Roselet, Paulo Cesar Abreu, Daniela Fernandes Ramos
Summary: This study aimed to characterize the main metabolites of three species of microalgae using H-1 HR-MAS NMR. Although the three species were found in the same ecosystem and had a high production of lipid compounds, significant differences were identified in the production of specialized metabolites. These unique properties make these compounds potential leaders in the development of new bioactive compounds for One Health and alternatives for aquaculture.
Article
Clinical Neurology
Leland G. Richardson, Julie J. Miller, Yosuke Kitagawa, Hiroaki Wakimoto, Bryan D. Choi, William T. Curry
Summary: Immunotherapy has shown promising potential in treating aggressive solid tumors, including those within the central nervous system. However, the presence of the oncometabolite 2-hydroxyglutarate (2HG) generated by mutant IDH has been identified as a potential barrier to current immunotherapeutic approaches.
NEUROSURGICAL FOCUS
(2022)
Article
Gastroenterology & Hepatology
Xuewei Bai, Hongyu Zhang, Yamei Zhou, Katsuya Nagaoka, Jialin Meng, Chengcheng Ji, Dan Liu, Xianghui Dong, Kevin Cao, Joud Mulla, Zhixiang Cheng, William Mueller, Amalia Bay, Grace Hildebrand, Shaolei Lu, Joselynn Wallace, Jack R. Wands, Bei Sun, Chiung-Kuei Huang
Summary: This study aimed to investigate the involvement of TET1, a 2-OG-dependent enzyme regulating 5hmC formation, in CCA progression. Up-regulation of TET1 was observed in CCA patients compared to noncancerous bile ducts, and treatment with TET1 cosubstrates promoted 5hmC formation and malignancy of CCA cells. Inhibiting TET1 activity suppressed 5hmC levels and CCA carcinogenesis, suggesting TET1 as a potential target for CCA treatment.
Article
Biophysics
Covadonga Lucas-Torres, Helene Roumes, Veronique Bouchaud, Anne-Karine Bouzier-Sore, Alan Wong
Summary: This study demonstrates the potential of using NMR spectroscopy to profile microgram-scale soft tissue biopsies, achieving high resolution and sensitivity through a unique HR-mu MAS probe. Detailed spatially resolved metabolic maps were obtained, allowing for the delineation of tumor lesions and offering new possibilities for tissue pathology analysis.
NMR IN BIOMEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Ying Wang, Hanbing Zhang, Minglei Wang, Jing He, Hui Guo, Lihua Li, Jie Wang
Summary: Through comparing gene expression profiles of glioma patients, the study identified CCNB2 as a key factor in malignant transformation of glioma, which induces SASP to mediate malignant progression. These findings reveal a previously undiscovered link between senescence, CCNB2/SASP/Cathepsin B & PGE2 axis and malignant transformation in glioma.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Genetics & Heredity
Dana W. Y. Tsui, Michael L. Cheng, Maha Shady, Julie L. Yang, Dennis Stephens, Helen Won, Preethi Srinivasan, Kety Huberman, Fanli Meng, Xiaohong Jing, Juber Patel, Maysun Hasan, Ian Johnson, Erika Gedvilaite, Brian Houck-Loomis, Nicholas D. Socci, S. Duygu Selcuklu, Venkatraman E. Seshan, Hongxin Zhang, Debyani Chakravarty, Ahmet Zehir, Ryma Benayed, Maria Arcila, Marc Ladanyi, Samuel A. Funt, Darren R. Feldman, Bob T. Li, Pedram Razavi, Jonathan Rosenberg, Dean Bajorin, Gopa Iyer, Wassim Abida, Howard I. Scher, Dana Rathkopf, Agnes Viale, Michael F. Berger, David B. Solit
Summary: This study found that cfDNA tumor fraction can help interpret negative cfDNA results and guide the selection of subsequent sequencing platforms most likely to identify clinically-relevant genomic alterations through analysis of plasma samples from 118 metastatic cancer patients.
Article
Medicine, General & Internal
Shi-Fang Zou, Lin Tao, Zhen-Chu Feng, Ji-Yan Wang, Lin Liu, Wen-Long Liang, Jie-Na Liu, Dan-Dan Xu, Jia-Yan Lin, Jian-Guo Zhang, Xi Chen
Summary: Elite biopsy demonstrates high diagnostic accordance rates, low pathological underestimation rates, low incidence of hematoma, and affordable cost.
ARCHIVES OF MEDICAL SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Shivani Singh Gaur, Uday S. Annapure
Summary: This report discusses the metabolites produced by Enterococcus strain E.villorum SB2, isolated from vaginal source. The bacteria were grown in different media and M17 medium was selected for metabolite production. HR-LCMS analysis revealed the production of various metabolites, including amino acids, organic acids, peptides, flavonoids, terpenoids, indole derivatives, antimicrobial compounds, and fatty acids. These findings open up new possibilities for industrial applications of the strain.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Bijaya Thapa, Azma Mareyam, Jason Stockmann, Bernhard Strasser, Boris Keil, Philipp Hoecht, Stefan Carp, Xianqi Li, Zhe Wang, Yulin Chang, Jorg Dietrich, Erik Uhlmann, Daniel P. Cahill, Tracy Batchelor, Lawrence Wald, Ovidiu C. Andronesi
Summary: This study aimed to develop an absolute quantification method for whole-brain MRSI in glioma patients, successfully achieving accurate metabolite quantification in healthy volunteers through the integration of ERETIC with receive arrays. However, there was a discrepancy between ERETIC and internal water quantification methods in glioma patients.
JOURNAL OF MAGNETIC RESONANCE IMAGING
(2022)
Article
Biochemistry & Molecular Biology
Martina Vermathen, Hendrik von Tengg-Kobligk, Martin Nils Hungerbuehler, Peter Vermathen, Nico Ruprecht
Summary: This study used high-resolution magic angle spinning (HR-MAS) NMR to analyze the metabolic profiles of non-small cell lung cancer (NSCLC) cells and their adaptations to cisplatin (cisPt) resistance. The findings suggest that characteristic metabolic patterns for cisPt resistance may serve as potential biomarkers in cancer medicine.
Article
Oncology
Xiaomin Li, Wanchun Zhang
Summary: The study explored the feasibility and safety of guided retroperitoneal puncture biopsy using fused 18 F-FDG PET/CT images. The results showed that the technique was accurate and successful in diagnosing retroperitoneal masses, providing clear pathological and immunohistochemical results. The procedure was safe, with no severe complications observed.
Article
Biochemistry & Molecular Biology
Caroline de S. Farias, Martins Dias de Cerqueira, Pio Colepicolo, Leonardo Zambotti-Villela, Luzimar G. Fernandez, Paulo R. Ribeiro
Summary: This study assessed the chemical composition and antioxidant activity of A. cochliacarpos extracts, finding that ethyl acetate extracts had higher antioxidant activity and phenolic content. Utilizing metabolite profiling and chemometrics, the research successfully differentiated the chemical composition of extracts based on extraction solvent and botanical part used, providing insights for drug discovery and development programs.
CHEMISTRY & BIODIVERSITY
(2021)
Article
Neurosciences
Shan Cheng, Liang Liu, Delin Wang, Yongdong Li, Suwen Li, Jiaqi Yuan, Shilu Huang, Zhipeng Xu, Bin Jia, Zhe Li, Jun Dong
Summary: This study revealed that transformed dendritic cells (t-DCs) maintained some dendritic cell-specific surface markers and exhibited decreased expression of immune-related costimulatory molecules under immunosuppressive conditions mediated by glioma stem cells (GSCs). The expression of PTX3 and its transcription factor ZNF148 was upregulated in t-DCs, promoting their proliferation and migration. The ZNF148/PTX3 axis may play a crucial role in regulating the malignant transformation of dendritic cells.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Oncology
Ye Cheng, Shuangshuang Song, Yukui Wei, Geng Xu, Yang An, Jie Ma, Hongwei Yang, Zhigang Qi, Xinru Xiao, Jie Bai, Lixin Xu, Zeliang Hu, Tingting Sun, Leiming Wang, Jie Lu, Qingtang Lin
Summary: This study established accurate diagnostic criteria for gliomas based on FET-PET and DWI, identifying correlations between different gene mutations and imaging values, providing higher diagnostic accuracy for high-grade gliomas with IDH1, hTERT, and EGFR mutations.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Haiyang Wang, Haoran Li, Qianqian Jiang, Xuchen Dong, Suwen Li, Shan Cheng, Jia Shi, Liang Liu, Zhiyuan Qian, Jun Dong
Summary: Recent studies have shown that Glioma Stem-like Cells (GSCs) can induce malignant transformation of fibroblasts (t-FBs) in the tumor microenvironment through the HOTAIRM1/miR-133b-3p/TGF beta axis. This finding could potentially provide a new target for treating gliomas.
FRONTIERS IN ONCOLOGY
(2021)
Article
Chemistry, Physical
Hikari A. I. Yoshihara, Emine Can, Magnus Karlsson, Mathilde H. Lerche, Juerg Schwitter, Arnaud Comment
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2016)
Article
Biophysics
Masoumeh Dehghani, Nicolas Kunz, Bernard Lanz, Hikari A. I. Yoshihara, Rolf Gruetter
NMR IN BIOMEDICINE
(2017)
Article
Biochemistry & Molecular Biology
Yuhei Takado, Tian Cheng, Jessica A. M. Bastiaansen, Hikari A. Yoshihara, Bernard Lanz, Mor Mishkovsky, Sylvain Lengacher, Arnaud Comment
ACS CHEMICAL NEUROSCIENCE
(2018)
Article
Radiology, Nuclear Medicine & Medical Imaging
Jessica A. M. Bastiaansen, Hikari A. I. Yoshihara, Andrea Capozzi, Juerg Schwitter, Rolf Gruetter, Matthew E. Merritt, Arnaud Comment
MAGNETIC RESONANCE IN MEDICINE
(2018)
Article
Multidisciplinary Sciences
Christine Gonzales, Hikari A. I. Yoshihara, Nahzli Dilek, Julie Leignadier, Melita Irving, Pascal Mieville, Lothar Helm, Olivier Michielin, Juerg Schwitter
Article
Physiology
Camille Ansermet, Gabriel Centeno, Sylviane Lagarrigue, Svetlana Nikolaeva, Hikari A. Yoshihara, Sylvain Pradervand, Jean-Luc Barras, Nicolas Dattner, Samuel Rotman, Francesca Amati, Dmitri Firsov
Article
Biophysics
Hikari A. I. Yoshihara, Jessica A. M. Bastiaansen, Magnus Karlsson, Mathilde H. Lerche, Arnaud Comment, Juerg Schwitter
NMR IN BIOMEDICINE
(2020)
Article
Multidisciplinary Sciences
Emine Can, Mor Mishkovsky, Hikari A. Yoshihara, Nicolas Kunz, Dominique-Laurent Couturier, Ulf Petrausch, Marie-Agnes Doucey, Arnaud Comment
SCIENTIFIC REPORTS
(2020)
Article
Chemistry, Physical
Alice Radaelli, Hikari A. Yoshihara, Hiroshi Nonaka, Shinsuke Sando, Jan Henrik Ardenkjaer-Larsen, Rolf Gruetter, Andrea Capozzi
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2020)
Article
Biophysics
Claudia C. Zanella, Andrea Capozzi, Hikari A. Yoshihara, Alice Radaelli, Adele L. C. Mackowiak, Lionel P. Arn, Rolf Gruetter, Jessica A. M. Bastiaansen
Summary: This study aims to characterize solutions containing endogenously occurring alternatives to pyruvate as UV-induced nonpersistent radical precursors for in vivo hyperpolarized MRI. It demonstrates the potential of alpha-ketovalerate and alpha-ketobutyrate as endogenous polarizing agents for radical-free hyperpolarized MRI, simplifying quality-control tests in clinical applications by enabling high polarization without radical filtration.
NMR IN BIOMEDICINE
(2021)
Article
Physiology
Hikari A. I. Yoshihara, Arnaud Comment, Juerg Schwitter
Summary: Measuring renal gluconeogenesis using invasive techniques is challenging, but less invasive methods like magnetic resonance spectroscopy can provide sensitive detection of metabolism. In fasted rats, pyruvate-to-bicarbonate conversion in the kidney is mainly through PDH flux, while aspartate conversion serves as a potential marker of renal gluconeogenesis following PEPCK inhibition.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biology
Emine Can, Jessica A. M. Bastiaansen, Dominique-Laurent Couturier, Rolf Gruetter, Hikari A. Yoshihara, Arnaud Comment
Summary: Can et al. demonstrate the use of hyperpolarized [1-13C]pyruvate and 13C magnetic resonance to directly assess liver enzymatic activities, allowing non-invasive detection of hepatic gluconeogenesis. They found that using two- to three-fold the basal plasma concentration of hyperpolarized [1-C-13]pyruvate enables measurement of liver metabolism. The study also reveals that supraphysiological doses of hyperpolarized [1-C-13]pyruvate can saturate or partially inhibit pyruvate dehydrogenase activity.
COMMUNICATIONS BIOLOGY
(2022)
Article
Urology & Nephrology
Camille Ansermet, Gabriel Centeno, Yohan Bignon, Daniel Ortiz, Sylvain Pradervand, Andy Garcia, Laure Menin, Frederic Gachon, Hikari Ai Yoshihara, Dmitri Firsov
Summary: This study aims to investigate the pathophysiological role of intrinsic circadian clocks in the kidney of diabetics. By inducing type I diabetes in mice, the researchers found that mice with diabetes exhibited exacerbated hyperglycemia, increased polyuria, and enhanced expression of the gluconeogenic pathway in the kidneys.
KIDNEY INTERNATIONAL
(2022)
Article
Multidisciplinary Sciences
Arianna Ferrari, Josh Peters, Mariia Anikeeva, Andrey Pravdivtsev, Frowin Ellermann, Kolja Them, Olga Will, Eva Peschke, Hikari Yoshihara, Olav Jansen, Jan-Bernd Hoevener
Summary: This article describes the setup, operational procedures, and performance of a cryogen-free device for producing hyperpolarized contrast agents in a preclinical imaging center. The polarization process was optimized to achieve a high polarization level. After injection, the distribution of different metabolites was observed using imaging techniques.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Alice Radaelli, Daniel Ortiz, Alessia Michelotti, Maxime Roche, Ryunosuke Hata, Shinsuke Sando, Olivier Bonny, Rolf Gruetter, Hikari A. I. Yoshihara
Summary: This study demonstrates the feasibility of using spin hyperpolarization to perform real-time metabolic imaging of carbon-13-labeled substrates. It shows that hyperpolarized L-(1-13C)alaninamide can serve as a probe to detect the activity of the cell-surface tumor marker aminopeptidase-N. Furthermore, it reveals that the chemical shift of alaninamide is pH-sensitive and can be used to measure the pH levels in different compartments.