Journal
NMR IN BIOMEDICINE
Volume 22, Issue 4, Pages 414-418Publisher
JOHN WILEY & SONS LTD
DOI: 10.1002/nbm.1351
Keywords
fMRI; isoflurane; somatosensory cortex; alpha-chloralose; forepaw stimulation; rats; repeated measures designs
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Isoflurane (ISO) can be a valuable alternative for a-chloralose (ACL) anesthesia in functional MRI (fMRI) studies. Therefore, we compared the efficacy of the blood oxygen level dependent (BOLD) effect in fMRI studies during ISO and ACL anesthesia sequentially in the same animals. After non-invasive instrumentation for ventilation and monitoring, series of T2* weighted MR images were acquired during forepaw stimulation, first under ISO, then followed by ACL anesthesia. The results demonstrated that ISO and ACL were both suitable to perform this fMRI experiment. The center of activation was at the same stereotactic position for both anesthetics and matched the primary somatosensory cortex (S1). Under the applied conditions, the BOLD response during ISO anesthesia declined in magnitude during the first stimulation period, as compared to ACL. From this study, we conclude that since ISO has several positive properties in comparison to ACL, including fast pharmacokinetics and suitability for repeated measurements, it is a valuable alternative for anesthesia in fMRl studies of rats. Copyright (C) 2008 John Wiley & Sons, Ltd.
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