Journal
STRUCTURE
Volume 23, Issue 7, Pages 1246-1257Publisher
CELL PRESS
DOI: 10.1016/j.str.2015.05.002
Keywords
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Funding
- DFG [SFB/B3]
- Medical Research Council [U105178939]
- Wellcome Trust
- EMBO short-term fellowship
- Medical Research Council [MC_U105178939] Funding Source: researchfish
- MRC [MC_U105178939] Funding Source: UKRI
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The TREX-2 complex integrates mRNA nuclear export into the gene expression pathway and is based on a Sac3 scaffold to which Thp1, Sem1, Sus1, and Cdc31 bind. TREX-2 also binds the mRNA nuclear export factor, Mex67: Mtr2, through the Sac3 N-terminal region (Sac3N). Here, we characterize Chaetomium thermophilum TREX-2, show that the in vitro reconstituted complex has an annular structure, and define the structural basis for interactions between Sac3, Sus1, Cdc31, and Mex67: Mtr2. Crystal structures show that the binding of C. thermophilum Sac3N to the Mex67 NTF2-like domain (Mex67 NTF2L) is mediated primarily through phenylalanine residues present in a series of repeating sequence motifs that resemble those seen in many nucleoporins, and Mlp1 also binds Mex67: Mtr2 using a similar motif. Deletion of Sac3N generated growth and mRNA export defects in Saccharomyces cerevisiae, and we propose TREX-2 and Mlp1 function to facilitate export by concentrating mature messenger ribonucleoparticles at the nuclear pore entrance.
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