Article
Biotechnology & Applied Microbiology
R. Aravintharaj, R. Asokan, K. Prasad Babu, M. Manamohan, Krishnan Nagendran
Summary: This study characterizes the Tomato spotted wilt virus (TSWV) isolate (Ka-To) infecting tomato from India through biological, serological, and molecular assays. The pathogenicity of TSWV (Ka-To) isolate was confirmed by mechanical inoculation on tomato, cowpea, and datura, resulting in necrotic or chlorotic local lesions. Serological assay and RT-PCR amplification confirmed the identity of TSWV. The obtained nucleotide sequences of Ka-To isolate showed similarity to TSWV isolates from Spain and Hungary, indicating reassortment and recombination in the genome.
Article
Multidisciplinary Sciences
Dongmei Liu, Muriel Mari, Xia Li, Fulvio Reggiori, Susan Ferro-Novick, Peter Novick
Summary: This study discovered a process called ER-phagy, which selectively delivers fragments of the endoplasmic reticulum to the lysosome or vacuole in response to starvation or the accumulation of misfolded proteins. The loss of specific genes and proteins blocks the association between the autophagy receptor and the assembly scaffold protein, and a membrane contact site module is also found to be involved in ER-phagy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Shiming Qi, Shijie Zhang, Md. Monirul Islam, Ahmed H. El-Sappah, Fei Zhang, Yan Liang
Summary: The Tomato spotted wilt virus (TSWV) is highly destructive to tomato cultivation globally, with only the natural resistance gene Sw-5 demonstrating high resistance. Over time, however, resistance induced by Sw-5 weakens due to the global spread of TSWV, necessitating the exploration of other resistance genes and novel resources for breeding TSWV-resistant tomato varieties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Wanhong Zhang, Yubing Jiao, Chengying Ding, Lili Shen, Ying Li, Yanbi Yu, Kun Huang, Bin Li, Fenglong Wang, Jinguang Yang
Summary: A sensitive detection system based on CRISPR/Cas13a was developed for rapid and accurate diagnosis of tomato spotted wilt virus (TSWV), demonstrating high selectivity and improved sensitivity compared to traditional RT-PCR methods.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Agronomy
Shiming Qi, Yuanbo Shen, Xinyu Wang, Shijie Zhang, Yushun Li, Md. Monirul Islam, Jin Wang, Pan Zhao, Xiangqiang Zhan, Fei Zhang, Yan Liang
Summary: The resistance gene Sl5R-1 interacts with the transcription factor SlTGA9 to regulate the SA and JA signaling pathways, providing resistance against Tomato spotted wilt virus.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Plant Sciences
Chinnaiah Senthilraja, Varagur Ganesan Malathi, Sevugapperumal Nakkeeran, Mariappan Suganthy, Uthandi Sivakumar, Perumal Renukadevi
Summary: Tomato spotted wilt virus (TSWV) infecting chrysanthemum in Southern India has been identified as a major constraint for chrysanthemum cut-flower industries. The virus was confirmed using TAS-ELISA, and host range experiments showed that Nicotiana tabacum had the highest virus titre. Genetic analysis revealed high similarity among isolates and their clustering with TSWV isolates from different countries.
CANADIAN JOURNAL OF PLANT PATHOLOGY
(2021)
Article
Virology
Hongwei Wang, Xiujuan Wu, Xiande Huang, Shujun Wei, Zhijun Lu, Jian Ye
Summary: This study provides evidence for the seed-borne transmission of Tomato spotted wilt orthotospovirus (TSWV) in pepper plants. The study shows that TSWV can be transmitted through seeds, with the endosperm being the main virus-containing seed organ. These findings are significant for researchers, certification agencies, the seed industry, and policy makers when evaluating the importance of TSWV in vegetable production worldwide.
Article
Plant Sciences
Jiansheng Guo, Guan Wang, Li Xie, Xinqiu Wang, Lingchong Feng, Wangbiao Guo, Xiaorong Tao, Bruno M. M. Humbel, Zhongkai Zhang, Jian Hong
Summary: This study utilized FIB-SEM technology to investigate the ultrastructural details of tomato spotted wilt virus (TSWV)-infected plant cells for the first time. It revealed several characteristic structures related to viral infection, such as the endoplasmic reticulum membrane network, lipid-like bodies, and connecting structures between cells.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Anja Konietzny, Jasper Grendel, Alan Kadek, Michael Bucher, Yuhao Han, Nathalie Hertrich, Dick H. W. Dekkers, Jeroen A. A. Demmers, Kay Gruenewald, Charlotte Uetrecht, Marina Mikhaylova
Summary: Excitatory synapses of principal hippocampal neurons are often found on dendritic spines, with dynamic strengthening or weakening of individual inputs leading to structural and molecular diversity of spines. Caldendrin, a Ca2+ sensor, regulates the localization of ER tubules and formation of the spine apparatus in dendritic spines through a myosin V-dependent pathway, transforming myosin into a stationary F-actin tether.
Review
Mycology
Qin-Rong Tan, Xiang-dong LI
Summary: This review summarizes the recent progress in fungal myosin-1 research, including its basic structure and function, comparison with vertebrate myosin-1, and its involvement in endocytosis and ER remodeling.
FUNGAL BIOLOGY REVIEWS
(2023)
Article
Entomology
Xiaobin Zheng, Yanran Wan, Min Tao, Jiangjiang Yuan, Kun Zhang, Jing Wang, Youjun Zhang, Pei Liang, Qingjun Wu
Summary: The Obst protein plays an important role in the acquisition and transmission of Tomato spotted wilt orthotospovirus (TSWV) in Frankliniella occidentalis, while the ApoD and Orai proteins do not have this effect.
Article
Agronomy
Tais M. F. Suassuna, Timothy Lane Grey, Xuelin Luo, Albert K. Culbreath, Cristiane Pilon
Summary: This study aimed to evaluate the vigor and germination of peanut seeds from different genotypes affected by Tomato spotted wilt disease (TSW). The results showed that TSW symptoms did not negatively affect the germination, vigor, and seed size of the seeds.
Article
Genetics & Heredity
Junheng Lv, Yunrong Mo, Minghua Deng, Junqiang Xu, Bin Xu, Xinyun Li, Jing Li, Caiqian Jiang, Ying Zhou, Ziran Wang, Zhengan Yang, Kai Zhao
Summary: In this study, a tomato inbred line, YNAU335, showed immunity to tomato spotted wilt virus (TSWV) at first but later lost its immunity. The researchers used various techniques to analyze the pathological features and genetic polymorphism of the TSWV infecting the tomato fruit. They discovered a mutated protein that likely caused the loss of immunity in YNAU335.
Article
Multidisciplinary Sciences
Jing Li, Si Chen, Run-Shuang Qiu, Li-Zhen Zhang, Yue Chen, Xue Zheng, Ting-Ting Li, Li-Hua Zhao, Zhong-Kai Zhang
Summary: This study found that the NSs protein of Tomato zonate spot virus acts as an RNA silencing suppressor in tobacco plants, inhibiting the transcription and protein expression of viral genes and alleviating the symptoms of viral infection. The study also discovered that the NSs protein suppresses the expression of the Zingipain-2-like protein, which co-localizes with NSs.
Article
Agriculture, Multidisciplinary
Baoan Song, Jiao Li, Ningning Zan, Hongfu He, Deyu Hu
Summary: A total of 35 piperazine derivatives were synthesized and assessed for their anti-tomato spotted wilt virus (TSWV) activity. Compounds 34 and 35 displayed significant potency against TSWV with EC50 values of 62.4 and 59.9 μg/mL, respectively, superior to the control agents ningnanmycin and ribavirin. Mechanistic studies revealed that compounds 34 and 35 inhibited TSWV infection by binding to the N protein, preventing assembly of the viral core structure ribonucleoprotein (RNP). Amino acid residue 94 on the N protein was identified as the key site for interaction between the compounds and the TSWV N target. This study highlights the potential of piperazine derivatives containing alpha-ketoamide moieties as antiviral agents.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)