Article
Infectious Diseases
Danxiao Wu, Xiaojuan Wang, Fangjun Feng, Dairong Wang, Yiqin Hu, Yang Yu, Jihong Huang, Min Wang, Jie Dong, Yaling Wu, Hong Zhu, Faming Zhu
Summary: The study identified varying non-discriminating reactive NAT yields and HBV NAT yields in different years, with HBV DNA viral load levels correlated with antibody levels. Donors with low viral loads pose risks in HBV NAT assays, leading to non-repeated NAT results during follow-up studies.
BMC INFECTIOUS DISEASES
(2021)
Article
Immunology
Xianlin Ye, Lihua Liu, Lina Chen, Xianghui Nie, Lu Huang, Denghuang Ye, Jinfeng Zeng, Tong Li, Bin Li, Min Xu, Limin Chen
Summary: This study analyzed the molecular characteristics and clinical outcomes of 70 OBIs identified in Heyuan city, Southern China. Most OBIs were genotype B, followed by genotype C, with various mutations affecting HBsAg detection observed in genotype B OBIs. Follow-up studies showed that most OBIs remained as OBIs, with some converting to chronic infections or recovery.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Jamel Groves, Roger Y. Dodd, Gregory A. Foster, Susan L. Stramer
Summary: Voluntary nonremunerated blood donors are at low risk for HCV infection, despite an increase in acute HCV infection in the general population. HCV subgenotypes 1a and 3a continue to remain predominant among US blood donors.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Virology
Pierre Cappy, Laure Boizeau, Daniel Candotti, Sophie Le Cam, Christophe Martinaud, Josiane Pillonel, Martin Tribout, Claude Maugard, Josiane Relave, Pascale Richard, Pascal Morel, Syria Laperche
Summary: Analysis of HBV infection data among blood donors in France showed a decreasing trend in the number of cases, with the main risk factors being the origin of donors and parenteral exposure. Screening strategies and pathogen-reduction measures help ensure blood safety.
Article
Immunology
Xianlin Ye, Yu Zhao, Ran Li, Tong Li, Xin Zheng, Wen Xiong, Jinfeng Zeng, Min Xu, Limin Chen
Summary: The study analyzed the presence of occult HBV infections in non-resolved blood donations in Chinese Blood Centers. It was found that 53.2% of non-resolved donations were anti-HBc+, and 40.5% of non-resolved pools contained OBIs, posing potential risk for HBV transmission.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Jianfeng Chen, Zili Ma, Dandan Wu, Qi Zuo, Fengtian Wang, Chen Xiao, Fuqiang Chen, Peng Li
Summary: This study evaluated the effectiveness of individual-donation nucleic acid testing (ID-NAT) in detecting occult hepatitis B virus infection (OBI) among anti-HBc positive blood donors, compared to minipool nucleic acid testing (MP-NAT). The results showed that using ID-NAT significantly increased the detection rate of HBV infection in anti-HBc positive donors, recommending independent HBV DNA testing for anti-HBc positive blood donors.
Review
Food Science & Technology
Lidong Pang, Xiaowen Pi, Xinyan Yang, Danliangmin Song, Xue Qin, Lihan Wang, Chaoxin Man, Yu Zhang, Yujun Jiang
Summary: This review article discusses the mechanisms and advantages/disadvantages of nucleic acid amplification (NAA)-based strategies for detecting foodborne pathogens in milk. It was found that NAA-based strategies, especially PCR, were effective in detecting foodborne pathogens in milk. LAMP showed high specificity, while RPA and RCA were suitable for on-site and in-situ detection, respectively, and enzyme-free amplification was more economical. However, factors such as sample separation, nucleic acid target conversion, and signal transduction affected the efficiency of NAA-based strategies. Further research is needed to simplify, integrate, and miniaturize microfluidic on-site detection platforms.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
Stefania Turrina, Davide Gibellini, Giacomo Giannini, Anna Lagni, Erica Diani, Virginia Lotti, Giulia Soldati, Filippo Gibelli, Dario Raniero, Domenico De Leo
Summary: This study evaluated the feasibility of using nucleic acid amplification techniques for detecting viral genomes in post-mortem samples, finding that viral genomes can be detected up to five days after death, providing an additional means for determining the cause of death in forensic cases.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2022)
Review
Infectious Diseases
Danxiao Wu, Fangjun Feng, Xiaojuan Wang, Dairong Wang, Yiqin Hu, Yang Yu, Jihong Huang, Min Wang, Jie Dong, Yaling Wu, Hong Zhu, Faming Zhu
Summary: This study assessed the results of nucleic acid amplification testing (NAT) screening for blood donations in Zhejiang province, China over a 10-year period. NAT screening was found to reduce the risk of transfusion-transmitted infections and various factors were found to influence the NAT yields of Hepatitis B virus (HBV), Hepatitis C virus (HCV), and Human immunodeficiency virus (HIV).
BMC INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Xianlin Ye, Tong Li, Ran Li, Heng Liu, Junpeng Zhao, Jinfeng Zeng
Summary: HBV infection poses a major threat to blood safety in high-prevalence countries like China. In Shenzhen, dual HBsAg ELISAs and NAT have been used for blood donors for over a decade. A small proportion of donors test HBsAg positive but NAT negative, leading to potential HBV infection with low viral load. The study aimed to investigate the molecular characteristics of HBV among blood donors testing HBsAg positive in ELISA tests. The results showed that most blood samples testing HBsAg positive in ELISA but NAT negative had viral nucleic acids detected, highlighting the need for more sensitive and specific assays for HBV detection among blood donors.
BMC INFECTIOUS DISEASES
(2021)
Article
Biophysics
Jiahao Li, Xuping Wu, Yue Li, Xin Wang, Huachuan Huang, Dan Jian, Yanke Shan, Yue Zhang, Chengcheng Wu, Guolei Tan, Shouyu Wang, Fei Liu
Summary: This smartphone-based HBV detecting technique offers a cost-effective, portable and user-friendly solution for HBV detection in underdeveloped areas. It has high sensitivity and accuracy, quantifies HBV viral load quickly, and has the potential for mobile HBV diagnoses and sharing epidemic information in resource-limited situations.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biochemical Research Methods
Ran Yan, Dawei Cai, Lea Ouyang, Richard Colonno, Qi Huang, Kathryn M. Kitrinos
Summary: Despite prolonged NrtI therapy, cure rates for HBV remain low due to persistent viral replication and inability to eliminate cccDNA. Recent studies suggest that combining serum HBV DNA levels with serum pgRNA levels may better predict liver-related complications. Current assays cannot monitor loss of residual virus in patients on NrtI therapy.
JOURNAL OF VIROLOGICAL METHODS
(2023)
Article
Fisheries
Fengqi Huang, Jinhong Shan, Kaishan Liang, Min Yang, Xiaoming Zhou, Xuzhuo Duan, Xianze Jia, Huihong Zhao, Qiwei Qin, Qing Wang
Summary: A rapid detection method for Red-spotted grouper nervous necrosis virus (RGNNV) based on the CRISPR-Cas13a system was developed in this study. The method showed high sensitivity and specificity, providing a theoretical basis for field detection of RGNNV.
Article
Immunology
Charitha Gowda, Stephanie Smith, Linda Crim, Katherine Moyer, Pablo J. Sanchez, Jonathan R. Honegger
Summary: This study aimed to investigate the sensitivity of modern HCV-RNA RT-PCR platforms for early diagnosis of perinatally acquired infection. The results showed that early HCV-RNA screening demonstrated 100% sensitivity and 100% specificity in detecting infected infants, highlighting its potential for aiding HCV surveillance and diagnosis in this population.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Hematology
Xuelian Deng, Xiaohan Guo, Tingting Li, Syria Laperche, Liang Zang, Daniel Candotti
Summary: Alternative viral particle concentration methods were used to confirm and characterize HBV infection status in NDR donors. The majority of NDR donors had occult HBV infections with extremely low viral DNA levels, which may pose a potential residual threat for blood safety.
Article
Hematology
Daniel Candotti, Virginie Sauvage, Pierre Cappy, Mohamed Abdallahi Boullahi, Pascal Bizimana, Guy Olivier Mbensa, Sekou Oumar Coulibaly, Aimee Olivat Rakoto Alson, Hadiza Soumana, Claude Tagny-Tayou, Edward L. Murphy, Syria Laperche
Article
Immunology
Mariama Sadjo Diallo, Assia Samri, Charlotte Charpentier, Melanie Bertine, Remi Cheynier, Rodolphe Thiebaut, Sophie Matheron, Fideline Collin, Martine Braibant, Daniel Candotti, Francoise Brun-Vezinet, Brigitte Autran
Summary: CD4 T cell subsets from HIV-1 LTNPs exhibit higher levels of activation, exhaustion, and SAMHD1 expression compared to those from HIV-2-infected individuals.
AIDS RESEARCH AND HUMAN RETROVIRUSES
(2021)
Article
Gastroenterology & Hepatology
Jingna Chen, Bochao Liu, Xi Tang, Xin Zheng, Jinhui Lu, Ling Zhang, Wenjing Wang, Daniel Candotti, Yongshui Fu, Jean-Pierre Allain, Chengyao Li, Linhai Li, Tingting Li
Summary: This study found that the W62R mutation in HBV Cp significantly reduces the production of HBcAg and HBeAg during HBV replication, potentially contributing to the occurrence of OBI.
JOURNAL OF HEPATOLOGY
(2021)
Article
Infectious Diseases
Jessica Vanhomwegen, Aurelia Kwasiborski, Abou Diop, Laure Boizeau, Damien Hoinard, Muriel Vray, Raymond Bercion, Babacar Ndiaye, Amelie Dublineau, Satoru Michiyuki, Jean-Claude Manuguerra, Virginie Sauvage, Daniel Candotti, Abdoulaye Seck, Syria Laperche, Yusuke Shimakawa
Summary: A simple, rapid, and inexpensive alternative to PCR, the HBV-LAMP assay, has been developed to diagnose high viraemia >= 200,000 IU/mL, which may help in identifying high-risk pregnant women in resource-limited countries to eliminate HBV mother-to-child transmission.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Review
Hematology
D. Candotti, C. Tagny-Tayou, S. Laperche
Summary: In Sub-Saharan Africa, challenges in blood screening for transfusion-transmitted infections persist due to limited resources and infrastructure, as well as affordability and sustainability concerns. Adequate facilities, quality management, and staff qualification are hindered by these limitations. Careful evaluation is needed for alternative blood screening strategies that consider local circumstances.
TRANSFUSION CLINIQUE ET BIOLOGIQUE
(2021)
Article
Oncology
Jun Huang, Lu Zhang, Shengjun Wu, Jie Lu, Fengying Li, Yulan Cheng, Qi Zhang, Guiling Li, Haitao Yu, Zhian Le, Xiaowen He, Yanjun Ding, Daniel Candotti, Xinyou Xie, Jun Zhang
Summary: The study conducted molecular and serological screening in a tertiary hospital in China to assess and limit the SARS-CoV-2 exposure risk from symptomless individuals. The results showed low prevalence and limited exposure risk, with varying seroprevalence depending on testing algorithm and confirmation criteria. Further investigation into protective immunity in asymptomatic COVID-19 patients who recovered is needed.
ANNALS OF TRANSLATIONAL MEDICINE
(2021)
Article
Gastroenterology & Hepatology
Xuelian Deng, Xiaohan Guo, Hongfang Gu, Dong Wang, Syria Laperche, Jean-Pierre Allain, Liang Zang, Daniel Candotti
Summary: Isolated anti-HBs occult hepatitis B virus infection in Chinese blood donors is associated with young, vaccinated adults who were exposed to HBV and predominantly develop low-level aborted infection. However, some individuals still experience low but persistent viral replication, and the clinical outcome remains uncertain.
JOURNAL OF VIRAL HEPATITIS
(2022)
Review
Virology
Xuelian Deng, Liang Zang, Daniel Candotti
Summary: The high prevalence of hepatitis B virus (HBV) infection in Chinese blood banks makes it a significant concern for blood safety. Highly sensitive screening methods have improved HBV blood safety, but result interpretation can be challenging. Procedures for the re-entry of temporarily deferred donors are needed to prevent donor losses and blood shortages. However, limited data and methods exist for HBV confirmation and donor follow-up.
Article
Virology
Pierre Cappy, Laure Boizeau, Daniel Candotti, Sophie Le Cam, Christophe Martinaud, Josiane Pillonel, Martin Tribout, Claude Maugard, Josiane Relave, Pascale Richard, Pascal Morel, Syria Laperche
Summary: Analysis of HBV infection data among blood donors in France showed a decreasing trend in the number of cases, with the main risk factors being the origin of donors and parenteral exposure. Screening strategies and pathogen-reduction measures help ensure blood safety.
Editorial Material
Virology
Daniel Candotti
Article
Immunology
Xi Tang, Liu Yang, Panli Zhang, Cong Wang, Shengxue Luo, Bochao Liu, Yongshui Fu, Daniel Candotti, Jean-Pierre Allain, Ling Zhang, Chengyao Li, Tingting Li
Summary: Hepatitis B surface antigen-negative, hepatitis B virus DNA-positive occult hepatitis B infection is associated with liver fibrosis, and hepatitis B core antigen is correlated with elevated serum liver fibrosis indexes and may affect fibrotic progression in liver tissue.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Hematology
Daniel Candotti, Steven J. Drews, Helen M. Faddy
Summary: The study aimed to determine the value that blood operators globally place on viral diversity surveillance and assess its impact. A questionnaire was circulated to members of the International Society of Blood Transfusion-transmitted infectious diseases working party. The results showed that 97% of respondents viewed monitoring viral genomic sequences as important, but only 47% were currently doing it. Lack of financial resources and expertise were reported as the main limitations.
Article
Virology
Xuelian Deng, Dan Liu, Maelenn Pailine Delcourt, Huihui Gao, Lu Zhou, Daniel Candotti
Summary: Between 2011 and 2021, a total of 869,633 blood donations were collected from prequalified donors in Dalian, China. Only 0.12% of the samples were confirmed to be positive for hepatitis B virus (HBV). Testing for hepatitis delta virus (HDV) antibodies in 2175 donations revealed that only 0.09% of the samples showed weak reactivity, but they were confirmed to be false positives upon repeat testing. Among the confirmed occult HBV infection (OBI) samples, only one non-Chinese donor showed repeated reactivity to HDV antibodies, suggesting that HDV/HBV coinfection does not play a significant role in the genesis of OBI. Overall, the data indicated a very low prevalence of HDV infection among blood donors in Liaoning province, Northeast China.
Article
Hematology
Pierre Cappy, Laure Boizeau, Daniel Candotti, Remi Caparros, Quentin Lucas, Eliane Garrabe, Christophe Martinaud, Sophie Le Cam, Pierre Gallian, Pascal Morel, Josiane Pillonel, Syria Laperche
Summary: The study analyzed the relevance of maintaining blood screening based on both HCV infection antibodies and nucleic acid testing in France. The results showed a decline in the yield of NAT-positive cases and an increase in donors with antibody-positive but NAT-negative results, implying a growing number of resolved infections. However, retesting revealed that at least 0.5% of antibody-positive but NAT-negative donors had low viral loads, indicating the potential risk of transmission. The findings suggest that retaining antibody screening in blood donation is still necessary in France.
Article
Hematology
Xuelian Deng, Xiaohan Guo, Tingting Li, Syria Laperche, Liang Zang, Daniel Candotti
Summary: Alternative viral particle concentration methods were used to confirm and characterize HBV infection status in NDR donors. The majority of NDR donors had occult HBV infections with extremely low viral DNA levels, which may pose a potential residual threat for blood safety.