Article
Reproductive Biology
Yao Xiao, Thiago F. Amaral, Pablo J. Ross, Delia A. Soto, Kenneth E. Diffenderfer, Aimee R. Pankonin, Surawich Jeensuk, Paula Tribulo, Peter J. Hansen
Summary: In cattle, inhibition of WNT signaling is crucial for the establishment of pluripotent embryonic stem cells, while JAK signaling pathway plays a role in maintaining pluripotency. IWR-1 can block the activation of STAT3, preventing differentiation of embryonic stem cells into a specific lineage.
BIOLOGY OF REPRODUCTION
(2021)
Article
Biology
Yao Xiao, Froylan Sosa, Pablo J. Ross, Kenneth E. Diffenderfer, Peter J. Hansen
Summary: Activin A stimulates NANOG expression while reducing SOX2 expression, and CHIR99021 suppresses both NANOG and SOX2 expression. Furthermore, activin A promotes blastocyst development, while CHIR99021 treatment impairs blastocyst formation and reduces the number of blastomeres.
Article
Biochemistry & Molecular Biology
Kilian Simmet, Mayuko Kurome, Valeri Zakhartchenko, Horst-Dieter Reichenbach, Claudia Springer, Andrea Bahr, Helmut Blum, Julia Philippou-Massier, Eckhard Wolf
Summary: The OCT4 gene is found to be crucial for the maintenance of pluripotency of the epiblast and differentiation of the hypoblast in bovine embryos.
Article
Developmental Biology
Natalia Lopez-Anguita, Seher Ipek Gassaloglu, Maximilian Stoetzel, Adriano Bolondi, Deniz Conkar, Marina Typou, Reneprime Buschow, Jesse V. Veenvliet, Aydan Bulut-Karslioglu
Summary: The microenvironment, particularly hypoxia, plays a crucial role in shaping the characteristics and differentiation potential of stem cells. This study reveals that acute and prolonged hypoxia have a temporal and cell type-specific transcriptional effect on embryonic and extra-embryonic stem cells, and can enhance lineage representation in a three-dimensional differentiation model.
Article
Cell & Tissue Engineering
Chunhao Deng, Zhaoying Zhang, Faxiang Xu, Jiaqi Xu, Zhili Ren, Carlos Godoy-Parejo, Xia Xiao, Weiwei Liu, Zhou Zhou, Guokai Chen
Summary: In this study, the impact of T3 on stem cell culture and lineage-specific differentiation was investigated using human pluripotent stem cells (hPSCs). T3 was found to enhance stem cell maintenance, improve culture consistency, modulate cellular metabolism, and promote energy production. The hormone also supported pluripotency by promoting specific signaling pathways and reduced dependence on certain growth factors. Furthermore, T3 significantly enhanced trophoblast differentiation under a specific induction condition. These findings highlight the potential role of T3 in improving stem cell applications.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell & Tissue Engineering
Ayaka Yanagida, Daniel Spindlow, Jennifer Nichols, Anish Dattani, Austin Smith, Ge Guo
Summary: Researchers have developed a human embryo model called blastoid through self-organization, which can form blastocyst-like structures within a short period of time, displaying three tissue layers and mimicking natural blastocysts. Single-cell transcriptome analysis confirms the high fidelity of the blastoid model, providing a versatile and scalable experimental model for human embryo research.
Article
Biochemistry & Molecular Biology
Daniel Olivieri, Eleonora Castelli, Yumiko K. Kawamura, Panagiotis Papasaikas, Ilya Lukonin, Melanie Rittirsch, Daniel Hess, Sebastien A. Smallwood, Michael B. Stadler, Antoine H. F. M. Peters, Joerg Betschinger
Summary: Mouse embryonic stem cells are biased towards producing embryonic endoderm rather than extraembryonic endoderm, which is regulated by transcriptional repressors Hdac3 and Dax1. Pluripotency factors Nr5a2 and Esrrb promote cell type conversion, and perturbation of the barrier extends mESC potency. This study shows that transcriptional repressors stabilize pluripotency by biasing the equilibrium between embryonic and extraembryonic lineages in mESCs.
Article
Endocrinology & Metabolism
Erin R. Okawa, Manoj K. Gupta, Sevim Kahraman, Praneeth Goli, Masaji Sakaguchi, Jiang Hu, Kaiti Duan, Brittany Slipp, Jochen K. Lennerz, Rohit N. Kulkarni
Summary: Insulin and insulin-like growth factor-1 receptors are crucial for maintaining pluripotency and normal lineage development of stem cells. Dysregulation in the function and signaling of these receptors leads to upregulation of compensatory pathways to maintain pluripotency, while causing abnormalities in directed differentiation toward the three lineages.
MOLECULAR METABOLISM
(2021)
Review
Biochemistry & Molecular Biology
Nazmus Salehin, Hilary Knowles, V. Pragathi Masamsetti, Patrick P. L. Tam
Summary: This review focuses on the current understanding of the interplay between signaling input and downstream transcriptional activity in the differentiation of germ layer tissue and cell lineages during gastrulation. It highlights the conservation and divergence of cellular and molecular mechanisms in the development of germ layers, with a particular emphasis on human embryo.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2022)
Article
Biochemistry & Molecular Biology
Ayaka Yanagida, Elena Corujo-Simon, Christopher K. Revell, Preeti Sahu, Giuliano G. Stirparo, Irene M. Aspalter, Alex K. Winkel, Ruby Peters, Henry De Belly, Davide A. D. Cassani, Sarra Achouri, Raphael Blumenfeld, Kristian Franze, Edouard Hannezo, Ewa K. Paluch, Jennifer Nichols, Kevin J. Chalut
Summary: This study investigates the mechanical basis of spatial segregation between the primitive endoderm and the epiblast. It proposes that differential surface fluctuations and cellular fluidity play a crucial role in ensuring physical lineage sorting.
Article
Cell Biology
Sung-Eun Kim, Pooja J. Chothani, Rehana Shaik, Westley Pollard, Richard H. Finnell
Summary: Our study reveals the crucial role of Gpr161 in the development of the posterior neural tubes, cranial neural crest, somite-derived skeletogenesis, and midbrain morphogenesis in mice.
DISEASE MODELS & MECHANISMS
(2023)
Article
Agriculture, Dairy & Animal Science
Mingyun Lee, Jong-Nam Oh, Gyung Cheol Choe, Kwang-Hwan Choi, Dong-Kyung Lee, Seung-Hun Kim, Jinsol Jeong, Yelim Ahn, Chang-Kyu Lee
Summary: This study determined the lineage-specific role of NANOG in parthenogenetic porcine blastocysts and found that NANOG plays an important role in the differentiation of the epiblast and primitive endoderm, and is essential for maintaining pluripotency within the epiblast.
Review
Biochemistry & Molecular Biology
Tabinda Sidrat, Zia-Ur Rehman, Myeong-Don Joo, Kyeong-Lim Lee, Il-Keun Kong
Summary: The Wnt/beta-catenin signaling pathway is crucial in early embryonic development, regulating cell proliferation and maintaining ESCs in a pluripotent state. Dysregulation of this pathway can lead to hereditary diseases and abnormalities. The interaction of Wnt signaling with other molecules and with PPAR delta is important in embryonic development and gene expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Chao Liu, Lingang Sun, Yijun Tan, Qi Wang, Tao Luo, Chenlu Li, Nan Yao, Yuting Xie, Xiao Yi, Yi Zhu, Tiannan Guo, Junfeng Ji
Summary: USP7 plays an important role in maintaining the identity of mouse embryonic stem cells (mESCs) by regulating the transcriptional network. It represses lineage differentiation genes through both catalytic activity-dependent and -independent mechanisms, and stabilizes the transcription factor SOX2 to suppress the expression of mesoendodermal lineage genes. Additionally, it contributes to Polycomb chromatin-mediated repression of lineage genes.
Article
Multidisciplinary Sciences
Andy Chan, Sophia Ma, Bret J. Pearson, Danny Chan
Summary: The extracellular matrix plays a crucial role in the maintenance, regeneration, and differentiation of stem cells within their niche. By studying the collagen gene family in the freshwater planarian Schmidtea mediterranea, researchers have identified a key gene, col4-1, which influences the proliferation of neoblasts. Furthermore, evidence suggests that an interaction between type IV collagen, the discoidin domain receptor, and neuregulin-7 regulate the balance of symmetric and asymmetric division of neoblasts through the NRG-7/EGFR pathway.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Engineering, Electrical & Electronic
Aleksandra Pokrzywnicka, Patrycja Sniadek, Natalia Malyszka, Danylo Lizanets, Wojciech Kubicki, Piotr Pawlak, Rafal Walczak
JOURNAL OF MICROMECHANICS AND MICROENGINEERING
(2019)
Article
Agriculture, Dairy & Animal Science
P. Racewicz, M. Majewski, Z. E. Madeja, A. Lukomska, M. Kubiak
BRITISH POULTRY SCIENCE
(2020)
Article
Reproductive Biology
Piotr Pawlak, Natalia Malyszka, Izabela Szczerbal, Pawel Kolodziejski
BIOLOGY OF REPRODUCTION
(2020)
Article
Developmental Biology
Ewelina Warzych, Piotr Pawlak, Dorota Lechniak, Zofia Eliza Madeja
DEVELOPMENTAL BIOLOGY
(2020)
Article
Agriculture, Dairy & Animal Science
Magdalena Bryszak, Malgorzata Szumacher-Strabel, Haihao Huang, Piotr Pawlak, Dorota Lechniak, Pawel Kolodziejski, Yulianri Rizki Yanza, Amlan Kumar Patra, Zora Varadyova, Adam Cieslak
ANIMAL FEED SCIENCE AND TECHNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Arkadiusz Kajdasz, Ewelina Warzych, Natalia Derebecka, Zofia E. Madeja, Dorota Lechniak, Joanna Wesoly, Piotr Pawlak
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Genetics & Heredity
Paulina Lipinska, Ewa Sell-Kubiak, Piotr Pawlak, Zofia Eliza Madeja, Ewelina Warzych
Summary: The experiment aimed to investigate the effects of selective inhibition of glucose or fatty acid metabolism on in vitro maturation of bovine cumulus-oocyte complexes (COCs). The results showed that only glucose metabolism was crucial to oocyte development, as inhibition of glucose decreased embryo culture efficiency. Different responses to inhibition were observed in cumulus cells, with upregulation of ketone body metabolism suggesting unsuccessful attempts to switch to lipid consumption. Treatment with etomoxir did not affect embryo development, indicating undisturbed metabolism in cumulus cells.
Article
Biochemistry & Molecular Biology
Zofia E. Madeja, Marta Podralska, Agnieszka Nadel, Marcin Pszczola, Piotr Pawlak, Natalia Rozwadowska
Summary: Mitochondrial content and activity can serve as reliable markers for evaluating bovine sperm quality, with motile sperm having more mitochondria and mtDNA copies, as well as higher mitochondrial membrane potential.
Article
Agriculture, Dairy & Animal Science
Piotr Pawlak, Ewelina Warzych, Zofia E. Madeja, Tomasz Nowak, Dorota Lechniak
Summary: The study found that oocytes from bitches with CEH/P have higher mtDNA content but do not show a negative impact on oocyte quality as reflected by the two parameters examined.
ANNALS OF ANIMAL SCIENCE
(2021)
Review
Developmental Biology
Zofia E. Madeja, Piotr Pawlak, Anna Piliszek
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
(2019)