Article
Biology
Yasunari Seita, Keren Cheng, John R. McCarrey, Nomesh Yadu, Ian H. Cheeseman, Alec Bagwell, Corinna N. Ross, Isamar Santana Toro, Li-hua Yen, Sean Vargas, Christopher S. Navara, Brian P. Hermann, Kotaro Sasaki
Summary: This study successfully established a method for culturing mid-gestation mouse iPSCs on a feeder layer, which can further differentiate into cells similar to primordial germ cells (PGCLCs). Furthermore, these cells can acquire an early developmental germ cell phenotype when co-cultured with mouse testicular somatic cells.
Article
Engineering, Biomedical
Bo Wang, Shoichi Iriguchi, Masazumi Waseda, Norihiro Ueda, Tatsuki Ueda, Huaigeng Xu, Atsutaka Minagawa, Akihiro Ishikawa, Hisashi Yano, Tomoko Ishi, Ryoji Ito, Motohito Goto, Riichi Takahashi, Yasushi Uemura, Akitsu Hotta, Shin Kaneko
Summary: The study demonstrates the generation of hypoimmunogenic cancer-antigen-specific T cells derived from human induced pluripotent stem cells, which escape rejection in allogeneic mice due to lacking certain components of the human leukocyte antigen system and incorporating a ligand that inhibits natural killer cells. These T cells also showed resistance to recognition by immune cells and maintained anti-tumor potency, highlighting their potential contribution to off-the-shelf T cell immunotherapies.
NATURE BIOMEDICAL ENGINEERING
(2021)
Article
Cell & Tissue Engineering
Sheng Yang, Zhen Liu, Shengda Wu, Lang Zou, Yanpei Cao, Hongjia Xu, Jingfeng Huang, Qingyan Tian, Fanggui Wu, Panpan Li, Shuping Peng, Cijun Shuai
Summary: This study successfully induced primordial germ cells and oocytes from human iPSCs, and facilitated oocyte differentiation through activation of the Wnt signaling pathway and reconstruction of ovarian nests.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Mehrafarin Kiani, Mansoureh Movahedin, Iman Halvaei, Masoud Soleimani
Summary: This study explores a new protocol for differentiating human induced pluripotent stem cells (hiPSCs) into primordial germ cell-like cells (PGCLCs). The results show that exposure to bone morphogenetic protein 4 (BMP4) and retinoic acid (RA) leads to significant changes in cell differentiation and gene expression. Immunofluorescence and RT-PCR analysis further confirm the formation of PGCLCs. The study suggests that EB formation and continuous exposure to BMP4 and RA can induce the differentiation of hiPSCs into PGCLCs.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Engineering, Biomedical
Ferdous B. Finklea, Yuan Tian, Petra Kerscher, Wen J. Seeto, Morgan E. Ellis, Elizabeth A. Lipke
Summary: The study demonstrates successful production of functional cardiac tissue microspheres through direct differentiation of hydrogel encapsulated hiPSCs, with high reproducibility and cell viability. These microspheres support efficient cardiac differentiation and have potential applications in biomanufacturing, drug screening, and regenerative therapies.
Article
Multidisciplinary Sciences
Christie Munn, Sarah Burton, Sarah Dickerson, Kiranmayee Bakshy, Anne Strouse, Deepika Rajesh
Summary: Macrophages generated from iPSCs offer a unique opportunity for genomic analysis and drug screening. The study demonstrates the scalability and cryopreservability of end stage macrophages derived from iPSCs, as well as the differential characteristics exhibited by macrophages generated from isogenic engineered clones. Generating a panel of functional iPSC-derived macrophages may provide insights into neural inflammatory responses associated with neurodegeneration.
Article
Cell & Tissue Engineering
Ana Belen Alvarez-Palomo, Jordi Requena-Osete, Raul Delgado-Morales, Victoria Moreno-Manzano, Carme Grau-Bove, Agueda M. Tejera, Manel Juan Otero, Carme Barrot, Irene Santos-Barriopedro, Alejandro Vaquero, Jovita Mezquita-Pla, Sebastian Moran, Carlos Hobeich Naya, Iris Garcia-Martinez, Francisco Vidal Perez, Maria A. Blasco, Manel Esteller, Michael J. Edel
Summary: The study demonstrates that utilizing synthetic mRNA transfection of CYCLIN D1 during reprogramming repairs DNA and significantly improves the genetic stability of human iPSC. This method reduces various genetic instabilities, decreases DNA damage, promotes correct protein expression, and reduces single-nucleotide polymorphism changes per chromosome.
Review
Immunology
Niclas Flosdorf, Martin Zenke
Summary: Novel methods of generating dendritic cells (DC) through reprogramming somatic cells have opened up exciting avenues for research and clinical applications. Induced pluripotent stem cells (iPS cells) and direct reprogramming of cells can both be used to generate DC.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Review
Cell & Tissue Engineering
Chunmeng Yao, Ruqiang Yao, Haining Luo, Ling Shuai
Summary: This review describes in vitro germline specification studies, focusing on the reconstitution of PGCs, meiosis, signaling pathways, and factors involved in these processes.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Endocrinology & Metabolism
Ila Tewari Jasra, Nerea Cuesta-Gomez, Kevin Verhoeff, Braulio A. Marfil-Garza, Nidheesh Dadheech, A. M. James Shapiro
Summary: This review summarizes the roles and mechanisms of mitochondria in somatic cell reprogramming to iPSCs and the metabolic shift associated with directed differentiation into pancreatic beta-like cells.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Shudong Zhao, Jishizhan Chen, Lei Wu, Xin Tao, Naheem Yaqub, Jinke Chang
Summary: Skeletal muscle, responsible for crucial functions in the body, can suffer from severe injuries leading to volumetric muscle loss (VML). Tissue-engineered muscles using induced pluripotent stem cells (iPSCs) offer a potential solution to restore muscle function. iPSCs, with their pluripotency and self-renewal capacity, can be used to construct tissue-engineered artificial skeletal muscles for various applications. These iPSC-based models also have the potential to revolutionize drug discovery and reduce reliance on animal models. This review provides an overview of iPSCs in tissue-engineered skeletal muscles, discussing advancements, challenges, and considerations for clinical translation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Shijia Liuyang, Guan Wang, Yanglu Wang, Huanjing He, Yulin Lyu, Lin Cheng, Zhihan Yang, Jingyang Guan, Yao Fu, Jialiang Zhu, Xinxing Zhong, Shicheng Sun, Cheng Li, Jinlin Wang, Hongkui Deng
Summary: We have developed a robust and chemically defined reprogramming protocol that significantly shortens the induction time for generating human pluripotent stem cells from somatic cells. This protocol promotes cell proliferation and metabolic activities, leading to a more direct reprogramming process.
Article
Chemistry, Multidisciplinary
Deogil Kim, Byung-Hyun Cha, Jinsung Ahn, Yoshie Arai, Bogyu Choi, Soo-Hong Lee
Summary: Understanding the biophysical relationships between stem cells and applied biomaterials is crucial in controlling stem cell functions. This study highlights the significant role of 3D microenvironment, particularly methacrylated hyaluronic acid (HA) hydrogel, in improving cellular reprogramming into iPSCs. The incorporation of cues from the 3D microenvironment accelerates the reprogramming process, offering potential advantages for translational applications.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Multidisciplinary Sciences
Jingyang Guan, Guan Wang, Jinlin Wang, Zhengyuan Zhang, Yao Fu, Lin Cheng, Gaofan Meng, Yulin Lyu, Jialiang Zhu, Yanqin Li, Yanglu Wang, Shijia Liuyang, Bei Liu, Zirun Yang, Huanjing He, Xinxing Zhong, Qijing Chen, Xu Zhang, Shicheng Sun, Weifeng Lai, Yan Shi, Lulu Liu, Lipeng Wang, Cheng Li, Shichun Lu, Hongkui Deng
Summary: This study demonstrates the chemical reprogramming of human somatic cells to human chemically induced pluripotent stem cells, which exhibit key features of embryonic stem cells. The induction of an intermediate plastic state and inhibition of the JNK pathway are crucial for successful chemical reprogramming.
Review
Cell Biology
Tae-Kyung Hong, Jae-Hoon Song, So-Been Lee, Jeong-Tae Do
Summary: Assisted reproductive technologies (ARTs) have limitations in rectifying germ cell aplasia such as non-obstructive azoospermia (NOA) and oocyte maturation failure syndrome. In vitro gametogenesis shows promise in overcoming infertility, with research focusing on deriving male and female germ cells from pluripotent stem cells (PSCs). This technique not only helps understand the biological mechanism of gamete development but also offers insights into its application in infertility treatments.
Article
Pharmacology & Pharmacy
Han Xiao, Rui Zhang, Xiaobo Fan, Xinglu Jiang, Mingyuan Zou, Xuejiao Yan, Haiping Hao, Guoqiu Wu
Summary: The development of nanomedicine has made significant breakthroughs in cancer treatment, but the biocompatibility and targeted penetration of nanomaterials remain limitations. A bifunctional nanoparticle, synthesized through the self-assembly of intermediate filaments with RGD peptide and encapsulation of 7-AAD molecules, serves as a fluorescent imaging probe for tumor treatment visualization.
ACTA PHARMACEUTICA SINICA B
(2022)
Review
Cell Biology
Xiao Zheng, Xiaoying Cai, Haiping Hao
Summary: The interaction between microbial metabolites and host targets plays a crucial role in signal transmission and biological regulation. Understanding the interaction between microbial metabolites and host targets can provide new insights into host health and disease, expand the range of druggable targets, and inspire drug design and discovery.
Article
Food Science & Technology
Keisuke Hamada, Ping Wang, Yangliu Xia, Nana Yan, Shogo Takahashi, Kristopher W. Krausz, Haiping Hao, Tingting Yan, Frank J. Gonzalez
Summary: WA has therapeutic effects on alcoholic liver disease by inhibiting hepatic lipogenesis to reduce liver injury caused by alcohol.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Biochemical Research Methods
Rongfan Tang, Pengcheng Chen, Zhe Wang, Lingling Wang, Haiping Hao, Tingjun Hou, Huiyong Sun
Summary: “Drug design targeting protein-protein interactions associated with disease development is crucial. Besides interrupting interactions, stabilizing protein-protein interactions can also be beneficial for therapy. However, characterizing the stabilization effect of stabilizers is challenging, which highlights the importance of developing reliable computational strategies.”
BRIEFINGS IN BIOINFORMATICS
(2022)
Article
Gastroenterology & Hepatology
Tingting Yan, Yuhong Luo, Nana Yan, Keisuke Hamada, Nan Zhao, Yangliu Xia, Ping Wang, Changdong Zhao, Dan Qi, Shoumei Yang, Lulu Sun, Jie Cai, Qiong Wang, Changtao Jiang, Oksana Gavrilova, Kristopher W. Krausz, Daxesh P. Patel, Xiaoting Yu, Xuan Wu, Haiping Hao, Weiwei Liu, Aijuan Qu, Frank J. Gonzalez
Summary: Intestinal PPAR alpha signaling modulates fatty acid uptake through regulating FABP1 expression, contributing to NASH progression. Targeting this pathway could be a potential therapeutic strategy for NASH treatment.
Article
Chemistry, Medicinal
Yun Wang, Yuanyuan Xu, Xiaowei Xu, Hong Wang, Dong Wang, Wenchao Yan, Jiaying Zhu, Haiping Hao, Guangji Wang, Lijuan Cao, Jun Zhang
Summary: This study confirmed the beneficial effects of Ginkgo biloba leave extract (GbE) in alleviating hypercholesterolemia, inflammation, and atherosclerosis. GbE treatment reshaped the intestinal microbiota, downregulated proinflammatory cytokines, and enhanced the expression of tight junction proteins. It also reversed the dysfunction of microbial metabolic phenotypes and reduced atherosclerotic plaque areas. These findings provide important insights into the pharmacological mechanism underlying the antiatherogenic efficacy of GbE.
PHYTOTHERAPY RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Hai-Ping Hao, Fei Xia, Hong-Tong Bai, Xun-you Yan, Hong-xia Cui, Xiao-ming Xie, Lei Shi
Summary: This study investigates the effect of bio-control plant Litsea cubeba branch inter-row cover on soil bacterial community, soil-enriched metabolites, and soil mineral nutrition in tea plantation. The results show that Litsea cubeba inter-row cover can modify the soil microbial structure and diversity, antagonize the plant pathogen community, and improve the soil aluminum and phosphorus status.
ANNALS OF MICROBIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Jiajia Shen, Huimin Guo, Shijia Liu, Wei Jin, Zhi-Wei Zhang, Yong Zhang, Keanqi Liu, Shuying Mao, Zhihao Zhou, Lin Xie, Guangji Wang, Haiping Hao, Yan Liang
Summary: This study identifies the crucial role of intestinal flora and microbiota metabolites in the occurrence and treatment of ischaemic stroke. The accumulation of branched-chain amino acids is closely associated with gut microflora dysbiosis and neuroinflammation. Lactobacillus helveticus and Lactobacillus brevis colonization exhibit neuroprotective activity and alleviate the accumulation of branched-chain amino acids.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Tingting Yao, Heng Xiao, Hong Wang, Xiaowei Xu
Summary: Proteolysis-targeting chimera (PROTAC) is a promising molecule that degrades specific proteins through the ubiquitin-proteasome system. It has shown remarkable efficacy in various fields and opened up new avenues for biomedical research and development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Qinghua Wang, Zhe Wang, Sheng Tian, Lingling Wang, Rongfan Tang, Yang Yu, Jingxuan Ge, Tingjun Hou, Haiping Hao, Huiyong Sun
Summary: The mechanism of transcriptional regulation in nuclear receptors involves two main conformations, active and inactive. It is challenging to determine the molecular type of a ligand bound to the receptor's ligand-binding pocket (LBP) because agonists and antagonists bind to the same position. Therefore, precise and efficient methods are needed to distinguish between agonists and antagonists targeting the LBP of nuclear receptors.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2022)
Article
Pharmacology & Pharmacy
Shuang Cui, Huijian Hu, An Chen, Ming Cui, Xiaojie Pan, Pengfei Zhang, Guangji Wang, Hong Wang, Haiping Hao
Summary: The acetylation of FXR regulates its nucleocytoplasmic shuttling and degradation under conditions of liver injury, which limits the clinical benefits of FXR agonists. SIRT1 activators can reduce FXR acetylation and prevent its cytosolic degradation, and combining SIRT1 activators with FXR agonists shows promise in treating liver diseases.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Pharmacology & Pharmacy
Wenjie Lu, Lingfei Yu, Lujun Wang, Songlin Liu, Manman Li, Zijun Wu, Shengqi Chen, Rongfeng Hu, Haiping Hao
Summary: A metformin hydrochloride mucosal nanoparticles enteric-coated capsule (MH-MNPs-EC) was developed to improve the bioavailability and hypoglycemic effect duration of metformin hydrochloride for the treatment of type 2 diabetes. The capsule showed improved efficacy and prolonged residence time in the intestine compared to commercial metformin hydrochloride capsules.
Review
Chemistry, Medicinal
Kang Wang, Yuecan Zhang, Guangji Wang, Haiping Hao, Hong Wang
Summary: Nonalcoholic fatty liver disease is a common liver disease without approved pharmacotherapy. Farnesoid X receptor (FXR) is considered a promising therapeutic target, but currently developed drugs have shown limited efficacy in clinical trials. This review summarizes the development of the FXR agonist obeticholic acid and discusses its limitations in depth.
MEDICINAL RESEARCH REVIEWS
(2023)
Article
Psychology, Multidisciplinary
Haiping Hao, Xiaobao Li, Xi Shen, Hong Jiang, Haoyue Xiao, Houchao Lyu
Summary: This study examined the reciprocal relationship between future time perspective and self-control among adolescents. The results showed that future-negative and future-confusional were negatively related to self-control, while future-positive, future-perspicuity, future-perseverant, and future-planning were positively related to self-control. The study also found reciprocal relationships between future-confusional, future-planning, and self-control.
CURRENT PSYCHOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Jianbing Wu, Yihui Hu, Hui Ye, Sheng Zhang, Jie Zhu, Duorui Ji, Yihua Zhang, Ya Ding, Zhangjian Huang
Summary: This study presents the design and construction of novel coordinative nanoparticles that can achieve specific activation and cleavage reactions of two prodrugs in tumors, leading to improved therapeutic efficacy for triple-negative breast cancer.