☆ 4.6 Meeting Abstract

A new generation of glucose biosensors - engineering cellobiose dehydrogenase for increased direct electron transfer

NEW BIOTECHNOLOGY (2009)

Journal

NEW BIOTECHNOLOGY

Volume 25, Issue -, Pages S115-S115

Publisher

ELSEVIER SCIENCE BV

DOI: 10.1016/j.nbt.2009.06.401

Keywords

-

Categories

Biochemical Research Methods Biotechnology & Applied Microbiology

Ask authors/readers for more resources

Protocol

Community support

Reagent

Community support

Authors

I am an author on this paper

Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6

Not enough ratings

Secondary Ratings

Novelty

-

Significance

-

Scientific rigor

-

Rate this paper

Recommended

Article Electrochemistry

An Oxygen Insensitive Amperometric Glucose Biosensor Based on An Engineered Cellobiose Dehydrogenase: Direct versus Mediated Electron Transfer Responses

Kavita Jayakumar, Thomas M. B. Reichhart, Christopher Schulz, Roland Ludwig, Alfons K. G. Felice, Donal Leech

Summary: Cellobiose dehydrogenase (CDH) is a biocatalyst capable of oxidizing cellobiose and related carbohydrates to generate electrical current. A novel engineered CDH has shown improved performance as a bioelectrocatalyst in glucose biosensors.

CHEMELECTROCHEM (2022)

Add to Collection

Article Biochemistry & Molecular Biology

Development of a Versatile Method to Construct Direct Electron Transfer-Type Enzyme Complexes Employing SpyCatcher/SpyTag System

Takumi Yanase, Junko Okuda-Shimazaki, Ryutaro Asano, Kazunori Ikebukuro, Koji Sode, Wakako Tsugawa

Summary: The aim of this research is to develop a versatile method for creating a DET-type oxidoreductase complex based on the SpyCatcher/SpyTag technique. By combining SpyCatcher-fused heme c and SpyTag-fused non-DET-type oxidoreductases, DET-type oxidoreductase complexes with retained enzymatic activity and electron transfer ability to the electrode were successfully formed.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)

Add to Collection

Review Biochemical Research Methods

Biotic-abiotic hybrids for bioanalytics and biocatalysis

Oren Bachar, Roy Cohen, Matan M. Meirovich, Yifat Cohen, Omer Yehezkeli

Summary: The advances in biotic-abiotic interfaced systems have great potential in bioanalytics and biocatalysis applications. The combination of nanoelements' unique electronic and optic properties with the selectivity and catalytic abilities of biotic materials can lead to superior features. These new nanobiohybrids may contribute to addressing global challenges such as alternative energy utilization and affordable biodiagnostics and therapeutics tools.

CURRENT OPINION IN BIOTECHNOLOGY (2023)

Add to Collection

Article Biophysics

Engineering bio-interfaces for the direct electron transfer of Myriococcum thermophilum cellobiose dehydrogenase: Towards a mediator-less biosupercapacitor/biofuel cell hybrid

Xiaomei Yan, Jing Tang, Su Ma, David Tanner, Roland Ludwig, Jens Ulstrup, Xinxin Xiao

Summary: A novel three-dimensional graphene-based carbon electrode has been successfully utilized for immobilizing Myriococcum thermophilum cellobiose dehydrogenase. Detailed structural and electrochemical characterizations reveal favorable performance in glucose oxidation reactions. The constructed entirely DET-based enzymatic biofuel cell demonstrates excellent power output and reliability.

BIOSENSORS & BIOELECTRONICS (2022)

Add to Collection

Article Chemistry, Analytical

Nanostructured Interface Loaded with Chimeric Enzymes for Fluorimetric Quantification of Cyclosporine A and FK506

Paulina K. Wells, Oleh Smutok, Zhong Guo, Kirill Alexandrov, Evgeny Katz

Summary: Advances in protein engineering have made it possible to create protein biosensors as alternatives to analytical and diagnostic methods. This study focuses on the construction of a protein biosensor array using an artificial variant of PQQ-glucose dehydrogenase, which addresses the challenges of sensitivity, multiplexing, and manufacturability. By immobilizing GDH protein on silica nanoparticles and monitoring changes in fluorescence, a highly sensitive biosensor array with low background and a wide dynamic range has been successfully developed for quantitative detection of drugs in biological fluids.

ANALYTICAL CHEMISTRY (2022)

Add to Collection

Review Chemistry, Physical

Recent trends in enzyme engineering aiming to improve bioelectrocatalysis proceeding with direct electron transfer

Oleh Smutok, Taras Kavetskyy, Evgeny Katz

Summary: The article discusses the third generation electrochemical biosensors based on enzymatic DET ability, where the enzyme's capability is dependent on its electron transfer from native cofactor to a conductive surface. This can be achieved through natural sources or gene engineering techniques, facilitated by modern molecular genetics.

CURRENT OPINION IN ELECTROCHEMISTRY (2022)

Add to Collection

Article Biophysics

A cytochrome b-glucose dehydrogenase chimeric enzyme capable of direct electron transfer

Marie-Christin Viehauser, Erik Breslmayr, Stefan Scheiblbrandner, Franziska Schachinger, Su Ma, Roland Ludwig

Summary: The development of third generation biosensors relies on enzymes capable of direct electron transfer (DET), achieved by fusing a cytochrome domain to an enzyme. In this study, the chimeric enzyme NcCYT-GcGDH was successfully generated in large quantities, showing high catalytic activity and the ability to perform interdomain electron transfer and direct electron transfer via the fused cytochrome domain. The high current obtained on a simple, unmodified electrode at a relatively low redox potential provides a promising starting point for further optimization.

BIOSENSORS & BIOELECTRONICS (2022)

Add to Collection

Article Chemistry, Physical

Interdomain Linker of the Bioelecrocatalyst Cellobiose Dehydrogenase Governs the Electron Transfer

Lan Zhang, Christophe V. F. P. Laurent, Lorenz Schwaiger, Lushan Wang, Su Ma, Roland Ludwig

Summary: Direct bioelectrocatalysis involves efficient electron transfer between enzymes and electrodes without the use of redox mediators, and has applications in biosensors, biofuel cells, and bioelectrosynthesis. Cellobiose dehydrogenase (CDH) is a well-studied multidomain bioelectrocatalyst with a catalytic flavodehydrogenase domain and a mobile, electron-transferring cytochrome domain linked by a flexible linker. This study investigates the regulatory mechanism of the linker on electron transfer in CDH and proposes a function-based definition of the linker region. The findings provide insights into optimizing electron transfer rates in multidomain enzymes and enhancing their bioelectrocatalytic performance through rational design of the interdomain linker.

ACS CATALYSIS (2023)

Add to Collection

Article Multidisciplinary Sciences

Investigating lytic polysaccharide monooxygenase-assisted wood cell wall degradation with microsensors

Hucheng Chang, Neus Gacias Amengual, Alexander Botz, Lorenz Schwaiger, Daniel Kracher, Stefan Scheiblbrandner, Florian Csarman, Roland Ludwig

Summary: This study demonstrates the activity of Lytic polysaccharide monooxygenase (LPMO) on wood cell walls and suggests that even low levels of hydrogen peroxide can support the enzymatic degradation of wood biomass. LPMO plays a synergistic role in fungal extracellular catabolism and industrial biomass degradation.

NATURE COMMUNICATIONS (2022)

Add to Collection

Article Chemistry, Physical

Chimeric Cellobiose Dehydrogenases Reveal the Function of Cytochrome Domain Mobility for the Electron Transfer to Lytic Polysaccharide Monooxygenase

Alfons K. G. Felice, Christian Schuster, Alan Kadek, Frantisek Filandr, Christophe V. F. P. Laurent, Stefan Scheiblbrandner, Lorenz Schwaiger, Franziska Schachinger, Daniel Kracher, Christoph Sygmund, Petr Man, Petr Halada, Chris Oostenbrink, Roland Ludwig

Summary: This study elucidates the role of the cytochrome (CYT) domain of cellobiose dehydrogenase (CDH) in two electron transfer steps, revealing key factors and protein conformational changes in interdomain electron transfer.

ACS CATALYSIS (2021)

Add to Collection

Article Biophysics

Strategic design and improvement of the internal electron transfer of heme b domain-fused glucose dehydrogenase for use in direct electron transfer-type glucose sensors

Kohei Ito, Junko Okuda-Shimazaki, Katsuhiro Kojima, Kazushige Mori, Wakako Tsugawa, Ryutaro Asano, Kazunori Ikebukuro, Koji Sode

Summary: Redesigned mutant Pcyb-AfGDH enzymes exhibit faster intramolecular electron transfer rates and higher DET current values, showing potential for application in continuous glucose monitoring systems.

BIOSENSORS & BIOELECTRONICS (2021)

Add to Collection

Article Chemistry, Analytical

Direct Electron Transfer of Glucose Oxidase on Pre-Anodized Paper/Carbon Electrodes Modified through Zero-Length Cross-Linkers for Glucose Biosensors

Gilberto Henao-Pabon, Ning Gao, K. Sudhakara Prasad, XiuJun Li

Summary: A disposable paper-based glucose biosensor with direct electron transfer of glucose oxidase was developed using simple covalent immobilization. It exhibited high electron transfer rate and good affinity for glucose oxidase, while maintaining enzymatic activities. This low-cost glucose biosensor achieved a wide glucose detection range and remarkable selectivity, making it suitable for monitoring different stages of diabetes.

BIOSENSORS-BASEL (2023)

Add to Collection

Article Biophysics

Lab-made flexible third-generation fructose biosensors based on 0D-nanostructured transducers

Filippo Silveri, Davide Paolini, Flavio Della Pelle, Paolo Bollella, Annalisa Scroccarello, Yohei Suzuki, Eole Fukawa, Keisei Sowa, Cinzia Di Franco, Luisa Torsi, Dario Compagnone

Summary: We report a scalable benchtop method to fabricate sensitive and flexible fructose dehydrogenase amperometric biosensors using 0D-nanomaterials dispersed in water. The electrochemical platform was made using Stencil-Printing and insulated with xurography. Carbon black and mesoporous carbon were used as nanomaterials to enable efficient direct electron transfer between the enzyme and the transducer. The biosensors exhibited high sensitivity and selectivity for detecting D-fructose in various samples.

BIOSENSORS & BIOELECTRONICS (2023)

Add to Collection

Article Chemistry, Multidisciplinary

How Oxygen Binding Enhances Long-Range Electron Transfer: Lessons From Reduction of Lytic Polysaccharide Monooxygenases by Cellobiose Dehydrogenase

Zhanfeng Wang, Shishi Feng, Carme Rovira, Binju Wang

Summary: Long-range electron transfer between cellobiose dehydrogenase (CDH) and lytic polysaccharide monooxygenases (LPMOs) is mediated by the heme propionates of CDH and solvent waters. Oxygen binding to the copper center of LPMO is coupled with the long-range interprotein electron transfer, leading directly to the formation of LPMO-Cu-II-O-2(-) species. This newly uncovered electron transfer mechanism rationalizes experimental observations and may have implications for catalysis in LPMOs and other metalloenzymes with O-2- or CO-binding-enhanced long-range electron transfer processes.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

Add to Collection

Article Chemistry, Multidisciplinary

Engineering the Turnover Stability of Cellobiose Dehydrogenase toward Long-Term Bioelectronic Applications

Andreas F. Geiss, Thomas M. B. Reichhart, Barbara Pejker, Esther Plattner, Peter L. Herzog, Christopher Schulz, Roland Ludwig, Alfons K. G. Felice, Dietmar Haltrich

Summary: The study aimed to improve the turnover stability of Cellobiose dehydrogenase (CDH) through high-throughput enzyme engineering, identifying two mechanisms contributing to stability and selecting 11 improved CDH variants. These engineered CDH variants hold promise for application in continuous biosensors or biofuel cells.

ACS SUSTAINABLE CHEMISTRY & ENGINEERING (2021)

Add to Collection

Article Biotechnology & Applied Microbiology

Versatile Oxidase and Dehydrogenase Activities of Bacterial Pyranose 2-Oxidase Facilitate Redox Cycling with Manganese Peroxidase In Vitro

Peter L. Herzog, Leander Suetzl, Beate Eisenhut, Daniel Maresch, Dietmar Haltrich, Christian Obinger, Clemens K. Peterbauer

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2019)

Add to Collection

Article Biotechnology & Applied Microbiology

The GMC superfamily of oxidoreductases revisited: analysis and evolution of fungal GMC oxidoreductases

Leander Suetzl, Gabriel Foley, Elizabeth M. J. Gillam, Mikael Boden, Dietmar Haltrich

BIOTECHNOLOGY FOR BIOFUELS (2019)

Add to Collection

Article Biotechnology & Applied Microbiology

Expression of a leptospiral leucine-rich repeat protein using a food-grade vector in Lactobacillus plantarum, as a strategy for vaccine delivery

Chattip Suphatpahirapol, Thu-Ha Nguyen, Yada Tansiri, Yodying Yingchutrakul, Sittiruk Roytrakul, Supachai Nitipan, Worawidh Wajjwalku, Dietmar Haltrich, Siriwan Prapong, Suttipun Keawsompong

3 BIOTECH (2019)

Add to Collection

Article Agriculture, Multidisciplinary

Structural Comparison of Different Galacto-oligosaccharide Mixtures Formed by β-Galactosidases from Lactic Acid Bacteria and Bifidobacteria

Suwapat Kittibunchakul, Sander S. van Leeuwen, Lubbert Dijkhuizen, Dietmar Haltrich, Thu-Ha Nguyen

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2020)

Add to Collection

Review Microbiology

Glutamate Decarboxylase from Lactic Acid Bacteria-A Key Enzyme in GABA Synthesis

Ida Bagus Agung Yogeswara, Suppasil Maneerat, Dietmar Haltrich

MICROORGANISMS (2020)

Add to Collection

Article Microbiology

Genomic Comparison of Lactobacillus casei AP and Lactobacillus plantarum DR131 with Emphasis on the Butyric Acid Biosynthetic Pathways

Widodo Widodo, Aditya Lutfe Ariani, Donny Widianto, Dietmar Haltrich

Summary: Butyric acid is known for its anticarcinogenic and antioxidative properties. Local LAB strains Lactobacillus casei AP and Lactobacillus plantarum DR131 isolated from Indonesian infants' digestive tract and indigenous fermented buffalo milk, respectively, can produce butyric acid in vitro. However, the specific genes and metabolic pathways involved in this process are still unknown. Genomic sequencing of these strains revealed similarities with other strains, but also differences in the enzymes responsible for butyric acid synthesis. These findings provide valuable insights into the use of these LAB strains as probiotics.

MICROORGANISMS (2021)

Add to Collection

Article Chemistry, Multidisciplinary

Engineering the Turnover Stability of Cellobiose Dehydrogenase toward Long-Term Bioelectronic Applications

Andreas F. Geiss, Thomas M. B. Reichhart, Barbara Pejker, Esther Plattner, Peter L. Herzog, Christopher Schulz, Roland Ludwig, Alfons K. G. Felice, Dietmar Haltrich

Summary: The study aimed to improve the turnover stability of Cellobiose dehydrogenase (CDH) through high-throughput enzyme engineering, identifying two mechanisms contributing to stability and selecting 11 improved CDH variants. These engineered CDH variants hold promise for application in continuous biosensors or biofuel cells.

ACS SUSTAINABLE CHEMISTRY & ENGINEERING (2021)

Add to Collection

Article Microbiology

Analysis and Reconstitution of the Menaquinone Biosynthesis Pathway in Lactiplantibacillus plantarum and Lentilactibacillus buchneri

Nisit Watthanasakphuban, Ludovika Jessica Virginia, Dietmar Haltrich, Clemens Peterbauer

Summary: Respiratory metabolism has been reported in some lactic acid bacteria upon supplementation with only heme, resulting in enhanced biomass formation, reduced acidification, increased resistance to oxygen, and improved long-term storage. However, incomplete menaquinone biosynthesis pathway has been found in Lactobacillaceae, which needs further investigation and validation.

MICROORGANISMS (2021)

Add to Collection

Article Microbiology

Characterization of Fungal FAD-Dependent AA3_2 Glucose Oxidoreductases from Hitherto Unexplored Phylogenetic Clades

Sudarma Dita Wijayanti, Leander Suetzl, Adele Duval, Dietmar Haltrich

Summary: The CAZy auxiliary activity family 3 (AA3) comprises FAD-dependent enzymes belonging to the superfamily of GMC oxidoreductases, including glucose oxidase and glucose dehydrogenase. Recent phylogenetic analysis revealed new clades of AA3_2 glucose oxidoreductases with diverse substrate specificities and catalytic efficiencies. The newly studied AA3_2 glucose oxidoreductases showed a broader substrate spectrum than the archetypal GOx from Aspergillus niger.

JOURNAL OF FUNGI (2021)

Add to Collection

Article Chemistry, Multidisciplinary

Flavin Mononucleotide-Dependent L-Lactate Dehydrogenases: Expanding the Toolbox of Enzymes for L-Lactate Biosensors

Lidiia Tsvik, Beate Steiner, Peter Herzog, Dietmar Haltrich, Leander Suetzl

Summary: The development of L-lactate biosensors has been hindered by the lack of diversity in L-lactate-oxidizing enzymes used as bioelements. This study utilized sequence information from protein databases to identify a wider range of enzymes for L-lactate oxidation. Additionally, a new class of soluble and FMN-dependent L-lactate dehydrogenases was discovered. These findings open up new possibilities for the construction of second-generation biosensors and other biotechnological applications.

ACS OMEGA (2022)

Add to Collection

Article Biochemistry & Molecular Biology

Biochemical Characterization of Pyranose Oxidase from Streptomyces canus-Towards a Better Understanding of Pyranose Oxidase Homologues in Bacteria

Anja Kostelac, Leander Sutzl, Jolanta Puc, Valentina Furlanetto, Christina Divne, Dietmar Haltrich

Summary: This study reports the biochemical characterization of a pyranose oxidase (ScPOx) from Streptomyces canus. The results showed that ScPOx oxidizes monosaccharides and various C- and O-glycosides, and belongs to the same sequence space as the recently discovered FAD-dependent C-glycoside 3-oxidase.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

Add to Collection

Article Infectious Diseases

Leptospira borgpetersenii Leucine-Rich Repeat Proteins and Derived Peptides in an Indirect ELISA Development for the Diagnosis of Canine Leptospiral Infections

Sineenat Sripattanakul, Teerasak Prapong, Attapon Kamlangdee, Gerd Katzenmeier, Dietmar Haltrich, Ratchanee Hongprayoon, Siriwan Prapong

Summary: Researchers have developed an indirect ELISA assay for the diagnosis of canine leptospiral infections using peptides derived from a recombinant LRR protein of Leptospira. The study showed that four peptides had high specificity and sensitivity, making them suitable for detection of antibodies against Leptospira in Thai dogs.

TROPICAL MEDICINE AND INFECTIOUS DISEASE (2022)

Add to Collection

Article Infectious Diseases

Leptospiral Leucine-Rich Repeat Protein-Based Lateral Flow for Assessment of Canine Leptospiral Immunoglobulin G

Sineenat Sripattanakul, Kanpapat Boonchuay, Teerasak Prapong, Worawidh Wajjwalku, Gerd Katzenmeier, Dietmar Haltrich, Ratchanee Hongprayoon, Siriwan Prapong

Summary: The lateral flow strip test using the rhKU_Sej_LRR_2271 protein demonstrated good potential in detecting anti-leptospiral IgG in dogs, with a significant association with LipL32 ELISA. However, its sensitivity, specificity, and accuracy were relatively low.

TROPICAL MEDICINE AND INFECTIOUS DISEASE (2022)

Add to Collection

Article Biochemistry & Molecular Biology

Purification and Immobilization of Superoxide Dismutase Obtained from Saccharomyces cerevisiae TBRC657 on Bacterial Cellulose and Its Protective Effect against Oxidative Damage in Fibroblasts

Phitsanu Pinmanee, Kamonwan Sompinit, Angkana Jantimaporn, Mattaka Khongkow, Dietmar Haltrich, Thidarat Nimchua, Prakit Sukyai

Summary: This study purified SOD from Saccharomyces cerevisiae TBRC657, resulting in purified Mn-SOD with a molecular weight of 25 kDa. The purified SOD showed optimal activity at pH 7.0 and 35°C, but had poor stability under high temperatures. However, when immobilized on BC, the stability was enhanced. Immobilized SOD demonstrated the ability to regulate ROS levels inside cells, making it a promising candidate for antioxidant applications in various industries.

BIOMOLECULES (2023)

Add to Collection

Review Food Science & Technology

Recent Advances in Electrochemical Enzyme-Based Biosensors for Food and Beverage Analysis

Sudarma Dita Wijayanti, Lidiia Tsvik, Dietmar Haltrich

Summary: Food analysis and control are crucial for ensuring the quality and safety of food products. Electrochemical biosensors based on enzymes offer high selectivity, sensitivity, short analysis time, and cost-effectiveness, making them promising tools for food analysis.

FOODS (2023)

Add to Collection

No Data Available

© Peeref 2019-2024. All rights reserved.