Article
Chemistry, Multidisciplinary
Deogil Kim, Byung-Hyun Cha, Jinsung Ahn, Yoshie Arai, Bogyu Choi, Soo-Hong Lee
Summary: Understanding the biophysical relationships between stem cells and applied biomaterials is crucial in controlling stem cell functions. This study highlights the significant role of 3D microenvironment, particularly methacrylated hyaluronic acid (HA) hydrogel, in improving cellular reprogramming into iPSCs. The incorporation of cues from the 3D microenvironment accelerates the reprogramming process, offering potential advantages for translational applications.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Multidisciplinary Sciences
Md Emon Cevallos, Ricardo Raul Cevallos, Ruowen Zhang, Kejin Hu
Summary: Research has found that the generation of induced pluripotent stem cells (iPSCs) is inefficient and stochastic. The reasons for these deficiencies are not yet clear. It has been shown that the reprogramming factors, OCT4, SOX2, and KLF4, induce significant reprogramming stress, including transcriptional turbulence, stress-response gene deregulation, cell cycle impairment, downregulation of mitotic genes, illegitimate reprogramming, and cytotoxicity. However, the dominant-negative peptides of BET proteins enhance iPSC reprogramming and alleviate these reprogramming stresses. These peptides target a similar set of genes as BET chemical inhibitors, suggesting a distinct approach to targeting BET proteins.
Article
Cell & Tissue Engineering
Salma Alasmar, Jez Huang, Karishma Chopra, Ewa Baumann, Amy Aylsworth, Melissa Hewitt, Jagdeep K. Sandhu, Joseph S. Tauskela, Robert N. Ben, Anna Jezierski
Summary: This study investigated the role of ice recrystallization inhibitors (IRIs) as cryoprotectants for iPSCs and iPSC-derived neurons (iPSC-Ns). The results showed that IRIs, specifically 2FA, increased the post-thaw viability and recovery of iPSCs without affecting pluripotency. Although 2FA supplementation did not significantly improve iPSC-N cell post-thaw viability, 2FA cryopreserved iPSC-Ns exhibited better restoration of neuronal network activity and synaptic function compared to controls. This study highlights the importance of optimizing cryopreservation media formulations with IRIs to improve functional cryopreservation of iPSCs and post-mitotic iPSC-Ns.
Article
Neurosciences
Christian Schinke, Valeria Fernandez Vallone, Andranik Ivanov, Yangfan Peng, Peter Koertvelyessy, Luca Nolte, Petra Huehnchen, Dieter Beule, Harald Stachelscheid, Wolfgang Boehmerle, Matthias Endres
Summary: Chemotherapy-induced peripheral neuropathy (CIPN) is a common adverse effect with no predictive biomarkers or preventive treatments available. Using sensory neurons derived from induced pluripotent stem cells as an experimental model for CIPN, researchers found differential responses to neurotoxic drugs between cell lines from different healthy donors, showcasing potential for studying CIPN pathogenesis and neuroprotective treatments.
NEUROBIOLOGY OF DISEASE
(2021)
Review
Biochemistry & Molecular Biology
Li-Jie Hsu, Chao-Lin Liu, Ming-Ling Kuo, Chia-Ning Shen, Chia-Rui Shen
Summary: Cell therapy involves using cells selected, manipulated, and treated outside the body to treat or prevent human disease. iPSCs are ideal cell sources due to their unique characteristics. NK cells are effective tumor killers without causing GVHD, making them excellent candidates for cell therapy.
Article
Biochemistry & Molecular Biology
Chih-Hsien Wu, Hsuan-Hwai Lin, Yi-Ying Wu, Yi-Lin Chiu, Li-Yen Huang, Cheng-Chung Cheng, Chung-Chi Yang, Tsung-Neng Tsai
Summary: Coronary artery disease is a major global issue. Patients with coronary artery disease often develop peptic ulcers when receiving aspirin treatment, making panendoscopy with biopsy necessary for diagnosis and treatment. A protocol for generating iPSCs from gastric fibroblasts via biopsy was established, with successful differentiation into functional cardiomyocytes demonstrated. This study represents the first in vitro generation of iPSCs from gastric fibroblasts.
Article
Biochemistry & Molecular Biology
Martha E. Floy, Sophie E. Givens, Oriane B. Matthys, Taylor D. Mateyka, Charles M. Kerr, Alexandra B. Steinberg, Ana C. Silva, Jianhua Zhang, Ying Mei, Brenda M. Ogle, Todd C. McDevitt, Timothy J. Kamp, Sean P. Palecek
Summary: The study explores the developmental lineage and functional properties of cardiac fibroblasts derived from human pluripotent stem cells, highlighting their potential in modeling fibrotic diseases and regenerative therapies in the context of heart function. The differences in characteristics between epicardial and second heart field cardiac fibroblasts provide insights for in vitro model selection and therapeutic applications.
Article
Cell & Tissue Engineering
Sandhya Sriram, Nam-Young Kang, Subha Subramanian, Tannistha Nandi, Samydurai Sudhagar, Qiaorui Xing, Gerine Jin-Ling Tong, Allen Kuan-Liang Chen, Thekkeparambil Chandrabose Srijaya, Patrick Tan, Yuin-Han Loh, Young-Tae Chang, Shigeki Sugii
Summary: In this study, a novel BODIPY-derived fluorescent probe, BDL-E5, was identified to detect live human iPS cells at the early reprogramming stage, enabling early identification of reprogramming cells and improving the efficiency of iPS cell generation. RNA sequencing analysis revealed early reprogramming gene expression patterns, particularly involving CREB1, which was found to significantly impact reprogramming efficiency when overexpressed or knocked down. Overall, BDL-E5 provides a valuable tool for studying the early reprogramming pathway and enhancing the production of human iPS cells for clinical applications.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Endocrinology & Metabolism
Ila Tewari Jasra, Nerea Cuesta-Gomez, Kevin Verhoeff, Braulio A. Marfil-Garza, Nidheesh Dadheech, A. M. James Shapiro
Summary: This review summarizes the roles and mechanisms of mitochondria in somatic cell reprogramming to iPSCs and the metabolic shift associated with directed differentiation into pancreatic beta-like cells.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Daniel F. Kaemena, Masahito Yoshihara, Meryam Beniazza, James Ashmore, Suling Zhao, Marten Bertenstam, Victor Olariu, Shintaro Katayama, Keisuke Okita, Simon R. Tomlinson, Kosuke Yusa, Keisuke Kaji
Summary: CRISPR/Cas9-mediated genome-wide knockout screening identified 24 reprogramming roadblock genes, among which depletion of the predicted KRAB zinc finger protein Zfp266 consistently enhanced murine iPSC generation in various reprogramming settings. ZFP266 was found to bind to Short Interspersed Nuclear Elements (SINEs) near the binding sites of OCT4, SOX2, and KLF4, inhibiting chromatin opening. Replacement of the KRAB co-suppressor with co-activator domains transformed ZFP266 from an inhibitor to a potent facilitator of iPSC reprogramming. The interaction between SINE and KRAB-ZFP is proposed to be a critical regulator of chromatin accessibility for efficient cellular identity changes.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Maki Takeda, Emiko Ito, Kazumasa Minami, Akima Harada, Noriko Mochizuki-Oda, Yoshiki Sawa, Shigeru Miyagawa
Summary: Irradiation of hiPSC-CMs can reduce the number of undifferentiated hiPSCs and tumor formation risk, without affecting the growth and morphology of cardiomyocytes. Tumorigenicity tests in mice showed lower tumor formation frequency in the irradiation group compared to the control group.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Shoji Seki, Mami Iwasaki, Hiroto Makino, Yasuhito Yahara, Yoshitaka Miyazaki, Katsuhiko Kamei, Hayato Futakawa, Makiko Nogami, Nguyen Tran Canh Tung, Tatsuro Hirokawa, Mamiko Tsuji, Yoshiharu Kawaguchi
Summary: This study demonstrates the successful induction of nucleus pulposus (NP)-like cells using normal human dermal fibroblasts (NHDFs) through ectopic expression of specific genes in a three-dimensional culture system. These findings may contribute significantly to the development of effective strategies for treating intervertebral disc (IVD) diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Ana Belen Alvarez-Palomo, Jordi Requena-Osete, Raul Delgado-Morales, Victoria Moreno-Manzano, Carme Grau-Bove, Agueda M. Tejera, Manel Juan Otero, Carme Barrot, Irene Santos-Barriopedro, Alejandro Vaquero, Jovita Mezquita-Pla, Sebastian Moran, Carlos Hobeich Naya, Iris Garcia-Martinez, Francisco Vidal Perez, Maria A. Blasco, Manel Esteller, Michael J. Edel
Summary: The study demonstrates that utilizing synthetic mRNA transfection of CYCLIN D1 during reprogramming repairs DNA and significantly improves the genetic stability of human iPSC. This method reduces various genetic instabilities, decreases DNA damage, promotes correct protein expression, and reduces single-nucleotide polymorphism changes per chromosome.
Article
Medicine, General & Internal
Mehwish Zehravi, Mohsin Wahid, Junaid Ashraf
Summary: This study successfully generated DMD patient specific iPSCs from CD3(+)T cells using episomal reprogramming and confirmed their pluripotent state through immunofluorescence. Feeder-free and xeno-free culture conditions were utilized to obtain these iPSCs.
PAKISTAN JOURNAL OF MEDICAL SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Hyun Kyu Kim, Tae Won Ha, Man Ryul Lee
Summary: Cells are the basic units of organisms, with over 30 trillion cells generated from a single-cell fertilized egg through division and differentiation. Establishing the Human Cell Atlas aims to understand the mechanisms underlying diseases and cellular activities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Hematology
Marco Busnelli, Stefano Manzini, Matteo Chiara, Alice Colombo, Fabrizio Fontana, Roberto Oleari, Francesco Poti, David Horner, Stefano Bellosta, Giulia Chiesa
Summary: The study found that overexpression of ApoA-I in EKO mice aorta modulates the development of atherosclerosis, affecting pathways beyond those associated with inflammation and immune response.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Letter
Hematology
Marco Busnelli, Stefano Manzini, Alice Colombo, Fabrizia Bonacina, Giuseppe D. Norata, Elsa Franchi, Silvia Castiglioni, Christos Andronis, Eftychia Lekka, Eugenio Scanziani, Giulia Chiesa
THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Multidisciplinary Sciences
Stefano Manzini, Marco Busnelli, Alice Colombo, Elsa Franchi, Pasquale Grossano, Giulia Chiesa
Summary: Functional enrichment analysis is a method to extract biological insights from gene expression data by placing gene expression changes in a broader biological context using reference knowledge bases. reString is a cross-platform software that helps users retrieve functional enrichment results and generate customizable charts without requiring specific bioinformatics skills.
SCIENTIFIC REPORTS
(2021)
Article
Hematology
Marco Busnelli, Stefano Manzini, Alice Colombo, Elsa Franchi, Fabrizia Bonacina, Matteo Chiara, Francesca Arnaboldi, Elena Donetti, Federico Ambrogi, Roberto Oleari, Antonella Lettieri, David Horner, Eugenio Scanziani, Giuseppe Danilo Norata, Giulia Chiesa
Summary: This study found that apoA-I deficiency, even under normal lipid conditions, leads to HDL deficiency, causing skin morphological changes, atherosclerosis, and inflammation.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Article
Food Science & Technology
Marco Busnelli, Stefano Manzini, Alice Colombo, Elsa Franchi, Mitja Laaperi, Reijo Laaksonen, Giulia Chiesa
Summary: This study evaluates the effects of different diets on plasma and aorta lipidome in an athero-prone mouse model. Results show that the cholesterol and fatty acid content of the diets are the main factors affecting lipid accumulation in plasma and aorta. Furthermore, specific lipids such as lactosylceramide, glucosyligalactosylceramide, and individual ceramide species are found to accumulate in diseased aortic segments, serving as markers of atherosclerosis.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
M. Busnelli, E. Franchi, A. Colombo, S. Manzini, M. A. Garcia-Rivera, J. Kirwan, G. Chiesa
Meeting Abstract
Cardiac & Cardiovascular Systems
G. Chiesa, A. Colombo, E. Franchi, S. Manzini, M. A. Garcia-Rivera, J. Kirwan, M. Busnelli
Meeting Abstract
Cardiac & Cardiovascular Systems
A. Colombo, S. Manzini, M. Busnelli, E. Franchi, G. Chiesa
Meeting Abstract
Cardiac & Cardiovascular Systems
E. Franchi, M. Busnelli, S. Manzini, A. Colombo, G. Chiesa
Meeting Abstract
Cardiac & Cardiovascular Systems
S. Manzini, M. Busnelli, A. Colombo, E. Franchi, P. Grossano, G. Chiesa
Meeting Abstract
Biochemistry & Molecular Biology
Roberto Oleari, Antonella Lettieri, Stefano Manzini, Alyssa J. J. Paganoni, Paolo Grazioli, Marco Busnelli, Helen L. Storr, Valentina Massa, Sasha R. Howard, Anna Cariboni
EUROPEAN JOURNAL OF HUMAN GENETICS
(2022)
Meeting Abstract
Cardiac & Cardiovascular Systems
M. Busnelli, S. Manzini, A. Colombo, F. Fontana, R. Oleari, F. Poti, S. Castiglioni, S. Bellosta, G. Chiesa
Meeting Abstract
Cardiac & Cardiovascular Systems
G. Chiesa, S. Manzini, A. Colombo, E. Donetti, E. Scanziani, D. G. Norata, M. Busnelli
Meeting Abstract
Cardiac & Cardiovascular Systems
A. Colombo, S. Manzini, M. Busnelli, E. Scanziani, G. Chiesa
Meeting Abstract
Cardiac & Cardiovascular Systems
S. Manzini, M. Busnelli, A. Colombo, M. Kiamehr, G. Chiesa