Review
Oncology
Robert Wieder
Summary: Cancer cells metastasize to the bone marrow and remain dormant before recurring as incurable disease. Studying the interactions between cancer cells and bone marrow cells in the laboratory can provide insight into controlling this process.
Article
Biology
Zengdi Zhang, Zan Huang, Mohamed Awad, Mohammed Elsalanty, James Cray, Lauren E. Ball, Jason C. Maynard, Alma L. Burlingame, Hu Zeng, Kim C. Mansky, Hai-Bin Ruan
Summary: In mammals, the interaction between bone marrow stroma and hematopoietic progenitors contributes to bone-BM homeostasis, but the mechanisms and interactions between the development of skeletal and hematopoietic systems are largely unknown. This study reveals that the intracellular O-GlcNAc modification plays a role in dictating the fate and function of early BM stromal cells (BMSCs). O-GlcNAcylation promotes osteogenic differentiation of BMSCs and supports lymphopoiesis, while inhibiting adipogenesis and myelopoiesis. Impaired bone formation and defective hematopoiesis were observed when O-GlcNAc transferase (OGT) was ablated in BMSCs.
Article
Health Care Sciences & Services
Suncica Kapor, Milica Vukotic, Tijana Suboticki, Dragoslava Dikic, Olivera Mitrovic Ajtic, Milica Radojkovic, Vladan P. Cokic, Juan F. Santibanez
Summary: The study found that HU inhibits the growth of BMMSC, causing senescence-like changes and affecting cellular differentiation and immunoregulatory functions.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Chiara Valsecchi, Stefania Croce, Alice Maltese, Lorenza Montagna, Elisa Lenta, Alice Nevone, Maria Girelli, Paolo Milani, Tiziana Bosoni, Margherita Massa, Carlotta Abba, Rita Campanelli, Jessica Ripepi, Annalisa De Silvestri, Adriana Carolei, Giovanni Palladini, Marco Zecca, Mario Nuvolone, Maria Antonietta Avanzini
Summary: Immunoglobulin light-chain amyloidosis (AL) is characterized by misfolded light chains, and this study aimed to characterize bone marrow (BM)-MSCs from AL patients. The results showed that AL-MSCs were comparable to those from healthy donors in morphology and differentiation capacities, but had a reduced proliferation rate compared to healthy donor MSCs. Additionally, the modulatory effect of AL-MSCs on plasma cells was similar to that of healthy donor MSCs.
Article
Cell & Tissue Engineering
Feng Zhi, Yi Ding, Rong Wang, Yujiao Yang, Kaiming Luo, Fei Hua
Summary: Exosomal hsa_circ_0006859 is a potential biomarker for postmenopausal osteoporosis and regulates the balance between osteogenesis and adipogenesis in hBMSCs by sponging miR-431-5p.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Immunology
Mingjun Li, Xiangwen Shi, Yipeng Wu, Baochuang Qi, Chaoqun Zhang, Bin Wang, Bihuan Zhang, Yongqing Xu
Summary: This study explores the mechanisms of BMSCs injury and osteogenic differentiation inhibition in osteomyelitis, and identifies a potential therapeutic target. The results show that knocking down the Pmepa1 gene can alleviate infection-induced cell injury and promote osteoblast differentiation. The findings are supported by results from a rat model.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hannah L. L. Smith, Juliet C. C. Gray, Stephen A. A. Beers, Janos M. M. Kanczler
Summary: Osteosarcoma, a bone cancer typically found in adolescents, is closely related to the conversion of bone marrow during adolescence. This study compared the differentiation potential of bone marrow cells from different regions with osteosarcoma cell lines. The results showed distinct differences in the differentiation abilities of hematopoietic and adipocyte-rich bone marrow, which correlated with specific characteristics of the osteosarcoma cell lines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Victor Gustavo Balera Brito, Mariana Sousa Patrocinio, Ayna Emanuelli Alves Barreto, Sabrina Cruz Tfaile Frasnelli, Vanessa Soares Lara, Carlos Ferreira Santos, Sandra Helena Penha Oliveira
Summary: The study demonstrated that Telmisartan (TELM) inhibited osteogenic differentiation and mineralization of SHR BMSC, favoring an adipogenic phenotype due to Pparg upregulation.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Materials Science, Biomaterials
Ziyan Wang, Feixue Mi, Jinchen Li, Dandan Chen, Minmin Lin, Xinyu Wang, Siying Wu, Changfeng Wu, Chao Liu
Summary: Bone marrow stromal cells (BMSCs) have great potential in bone defect repair. By using semiconducting polymer dots (Pdots) as markers, we successfully selected BMSCs and evaluated their therapeutic efficacy in a bone defect model.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Cell & Tissue Engineering
Jeroen van de Peppel, Gerben J. Schaaf, Adriana Arruda Matos, Yuan Guo, Tanja Strini, Wenda Verschoor, Amel Dudakovic, Andre J. van Wijnen, Johannes P. T. M. van Leeuwen
Summary: This research suggests that the cell surface glycoprotein CD24 could serve as a selective biomarker for identifying subpopulations of BMSCs with varying osteogenic potential. CD24(pos) cells have increased expression of genes related to cell adhesion, motility, and extracellular matrix, while also showing reduced proliferation compared to CD24(neg) cells.
STEM CELLS AND DEVELOPMENT
(2021)
Article
Cell Biology
Angelica Giuliani, Maria Giulia Bacalini, Deborah Ramini, Emanuela Mensa, Chiara Giordani, Luciano Xumerle, Paolo Garagnani, Fabiola Olivieri, Antonio Domenico Procopio, Maria Rita Rippo, Jacopo Sabbatinelli
Summary: Bone marrow mesenchymal stromal cells (BMSCs) have the ability to self-renew and differentiate into adipocytes and osteoblasts, but the role of DNA methylation in their function and replicative senescence is still unclear. A genome-wide DNA methylation analysis revealed genome-wide hypomethylation and overlapping differentially methylated positions (DMPs) in replicative senescent BMSCs and HUVECs. Pathway analysis identified common pathways associated with senescence, insulin resistance, molecule adhesion, and Wnt/beta-catenin signaling. This study provides valuable insights into the mechanisms of replicative senescence and BMSC differentiation.
Article
Biotechnology & Applied Microbiology
Madhan Jeyaraman, Shiva Kumar Bingi, Sathish Muthu, Naveen Jeyaraman, Rathinavelpandian Perunchezhian Packkyarathinam, Rajni Ranjan, Shilpa Sharma, Saurabh Kumar Jha, Manish Khanna, Sree Naga Sowndary Rajendran, Ramya Lakshmi Rajendran, Prakash Gangadaran
Summary: Human bone marrow is a promising source of mesenchymal stromal cells (MSCs) for regenerative procedures involving cartilage and bone. However, the low proportion of MSCs in the bone marrow requires careful harvesting and processing techniques. There is currently a lack of consensus regarding the variables in the harvesting and processing technique of bone marrow aspiration concentrate (BMAC) and their impact on MSC yield.
BIOENGINEERING-BASEL
(2022)
Article
Endocrinology & Metabolism
Viktorija Avilkina, Damien Leterme, Guillaume Falgayrac, Jerome Delattre, Flore Miellot, Veronique Gauthier, Christophe Chauveau, Olfa Ghali Mhenni
Summary: This study demonstrates, for the first time, that the duration and severity of energy deficits are critical for changes in bone parameters, BMSC differentiation, and Sirt1 expression.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Haotian Qin, Jian Weng, Bo Zhou, Weifei Zhang, Guoqing Li, Yingqi Chen, Tiantian Qi, Yuanchao Zhu, Fei Yu, Hui Zeng
Summary: This study found that the concentration of 5 mM magnesium ions is the most conducive for angiogenesis and differentiation. By activating the Notch signaling pathway, magnesium ions up-regulate the expression levels of downstream genes such as Hes1 and Hes5, as well as angiogenesis-related genes including Hif-1 alpha and eNOS, thereby promoting the angiogenesis differentiation of BMSCs. Additionally, magnesium ion-induced differentiation enhances the migration and chemotaxis of BMSCs.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2023)
Article
Biophysics
I Garcia-Arnaez, F. Romero-Gavilan, A. Cerqueira, F. Elortza, M. Azkargorta, F. Munoz, M. Mata, J. J. Martin de Llano, J. Suay, M. Gurruchaga, I Goni
Summary: This study used proteomic and histological techniques to analyze the effects of Ca-doped biomaterials in vitro and in vivo and verify the correlation between the two systems. The results showed that the proteomic analysis of biomaterials in vitro is a useful tool for predicting their impact in vivo.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)