Journal
NEUROTOXICITY RESEARCH
Volume 18, Issue 2, Pages 187-199Publisher
SPRINGER
DOI: 10.1007/s12640-009-9120-3
Keywords
3,4-Methylenedioxymethamphetamine; Neurotoxicity; Minocycline; Interleukin-1 beta; Microglia; Nuclear factor kappaB
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Funding
- MCYT [SAF2007-65175]
- Plan Nacional sobre Drogas [PR75/06-15077]
- Ministerio de Sanidad [RTA-RD06/0001/006]
- UCM-CAM [CCG07-UCM/SAL-2588]
- Ministerio de Educacion y Ciencia
- Comunidad de Madrid
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3,4-Methylenedioxymethamphetamine (MDMA, 'ecstasy') is a selective 5-HT neurotoxin in rat brain which has been shown to produce acute neuroinflammation characterized by activation of microglia and release of interleukin-1beta (IL-1 beta). We aimed to determine whether or not minocycline, a semi-synthetic tetracycline antibiotic capable of inhibiting microglial activation, could prevent the inflammatory response and reduce the toxicity induced by MDMA. Adult male Dark Agouti rats were given minocycline twice a day for 2 days (45 mg/kg on the first day and 90 mg/kg on the second day; 12-h apart, i.p.). MDMA (12.5 mg/kg; i.p.) was given after the third minocycline injection and animals were killed either 1 h later for the determination of NF kappa B binding activity, 3 h later for the determination of IL-1 beta, 24 h later for the determination of microglial activation or 7 days later for the determination of [H-3]-paroxetine binding as a measure of 5-HT neurotoxicity. MDMA increased NF kappa B activation, IL-1 beta release and microglial activation both in the frontal cortex and in the hypothalamus and 7 days later produced a reduction in the density of 5-HT uptake sites in both these brain areas. Minocycline prevented the MDMA-induced increase in NF kappa B activation, IL-1 beta release and microglial activation in the frontal cortex and prevented the 5-HT neurotoxicity 7 days later. However, in the hypothalamus, in spite of preventing MDMA-induced microglial activation, minocycline failed to prevent MDMA-induced NF kappa B activation, IL-1 beta release and neurotoxicity. This suggests that the protective mechanism of minocycline against MDMA-induced neurotoxicity in frontal cortex involves inhibition of MDMA-induced NF kappa B activation possibly through a reduction in IL-1 beta signalling.
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