Article
Cell Biology
Diana Szucs, Vanda Miklos, Tamas Monostori, Melinda Guba, Aniko Kun-Varga, Szilard Poliska, Erika Kis, Balazs Bende, Lajos Kemeny, Zoltan Vereb
Summary: Adipose-derived mesenchymal stem cells are used in regenerative medicine for burns and ulcers treatment. Inflammatory microenvironment can induce different biological pathways in these cells as shown by gene expression analysis. The secretion of cytokines and wound healing assay further support the accelerated wound healing potential of these cells under inflammation conditions.
Article
Cardiac & Cardiovascular Systems
Holger Loerchner, Laia Canes Esteve, Maria Elisa Goes, Roxanne Harzenetter, Nathalie Brachmann, Praveen Gajawada, Stefan Guenther, Nicolas Doll, Jochen Poeling, Thomas Braun
Summary: This study reveals that TNF alpha recruits VEGFA-expressing neutrophils for muscle tissue revascularization through the CCL20-CCR6 axis. This finding provides a promising approach for revascularization, especially in diabetic conditions.
CIRCULATION RESEARCH
(2023)
Article
Hematology
Tiantian Chu, Shuhong Hu, Jiaqian Qi, Xueqian Li, Xiang Zhang, Yaqiong Tang, Meng Yang, Yang Xu, Chang-Geng Ruan, Yue Han, De-Pei Wu
Summary: This study investigated the effects of TNF alpha on megakaryocyte and platelet generation and found that TNF alpha had differential effects on their production at different concentrations.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Chemistry, Multidisciplinary
Sarah Belperain, Zi Yae Kang, Andrew Dunphy, Brandon Priebe, Norman H. L. Chiu, Zhenquan Jia
Summary: CNDs, as a novel form of nanoparticles, have shown potential anti-inflammatory properties in reducing TNF-alpha induced endothelial cell inflammation. This finding may open up a new direction for the treatment of cardiovascular diseases.
Article
Biochemistry & Molecular Biology
Eman I. Sobeh, Gamal El-ghannam, Reda M. S. Korany, H. M. Saleh, Souad A. Elfeky
Summary: This study reports the preparation of CUR/HA nanocomposite and its application in protecting male Wistar rats from the carcinogenic consequences of gamma irradiation. TEM and XRD analysis provided information on the morphology and structure of the nanosized HA nanoparticles and CUR/HA nanocomposite. Biochemical analysis and histological examinations demonstrated that CUR/HA nanocomposite injection did not significantly affect non-irradiation rats, but controlled the changes in pro-inflammatory cytokines, kidney function, and liver function when injected pre-irradiation. This research introduces a novel effective protection modality for the gamma irradiation hazard.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Food Science & Technology
Jong Yeon Park, Bich Hang Do, Ju-Seung Lee, Hyun Cheol Yang, Anh Ngoc Nguyen, Martin Krupa, Chong Jai Kim, Yeon Jin Jang, Han Choe
Summary: The study found that recombinant crotamine has significant antinociceptive and anti-inflammatory effects, which are not related to the opioid receptor. Additionally, the effects of intraperitoneal or intraplantar recombinant crotamine are related to TNF-alpha.
Review
Biochemistry & Molecular Biology
Kun Yu, Chengxin Yu, Liping Jiao, Kun Miao, Li Ni, Xiaoquan Rao, Ling Zhou, Chunxia Zhao
Summary: Myeloid-derived suppressor cells (MDSCs) are immature and heterogeneous myeloid cells with immunosuppressive functions, and they play important roles in cancer, chronic inflammatory diseases, and autoimmune disorders. Tumor necrosis factor alpha (TNF-alpha) regulates the accumulation and activation of MDSCs. In this review, the roles of TNF-alpha in the recruitment, immunosuppressive functions, and chemotaxis of MDSCs are summarized, and the potential therapeutic effects of TNF-alpha on these cells in tumor growth and inflammatory disorders are discussed.
Article
Chemistry, Multidisciplinary
Shuyue Wang, Xiaojie Shi, Jie Li, Qianping Huang, Qun Ji, Ying Yao, Tao Wang, Lili Liu, Min Ye, Yun Deng, Peixiang Ma, Hongtao Xu, Guang Yang
Summary: TNF-alpha inhibitors have been successful in treating autoimmune diseases, but current approved drugs are limited to biological macromolecules. This study utilizes a natural product-enriched DNA-encoded library to identify small molecules that interfere with the protein-protein interaction between TNF-alpha and its receptor. Kaempferol, a natural product identified in the library, not only interacts with TNF-alpha directly but also blocks its interaction with TNFR1, thereby attenuating TNF-alpha signaling. This research provides a promising starting point for the development of small molecule TNF-alpha inhibitors for clinical use.
Article
Immunology
Lin Feng, Yinan Ma
Summary: The consumption of a high-fat diet increases TNF-alpha levels in the spleen of rats, along with upregulation of TLR4 and NF-kappa B mRNAs and downregulation of alpha 7nAChR mRNA expression in splenic macrophages. Exercise intervention can counteract the detrimental effects of HFD on TNF-alpha levels in the spleen and prevent the abnormal expression of these genes in rat spleen macrophages.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biology
Chih-Kai Liao, Kuan-Min Fang, Hui-Ting Huang, Wen-Ruei Chang, Chao-Chi Chuang, Shun-Fen Tzeng
Summary: This study investigates the impact of peripheral inflammation on glioma growth, focusing on the activation of microglia. Findings suggest that aggravation of microglia activation by peripheral inflammatory challenge and a loss of TNFR2 function might lead to the promotion of glioma growth.
Article
Medicine, Research & Experimental
Wei-juan Li, Wen-ping Zhou, Xu-yong Li, Xiao-li Jiang, Yun-chao Deng, Jie Shen, Han Xie, Gang Tan, Ling Huang, Hui Zhang
Summary: This study found that LITAF protein may play a promoting role in the formation of atherosclerosis through the NF-kappa B pathway.
CURRENT MEDICAL SCIENCE
(2023)
Article
Multidisciplinary Sciences
Chiara Griseta, Petronilla Battista, Fabio Castellana, Isabella Colonna, Sabrina Sciarra, Roberta Zupo, Ilaria Bortone, Luisa Lampignano, Sarah Tirelli, Giuseppe Berardino, Anita Mollica, Madia Lozupone, Francesco Panza, Pietro Fiore, Brigida Minafra, Rodolfo Sardone
Summary: Growing evidence shows the impact of inflammation on brain aging and neurodegeneration. This study examines the connection between global cognitive function and executive function, and the inflammatory markers IL-6, CRP, and TNF-alpha. The study, which involved a population-based sample of older adults in Southern Italy, measured blood levels of IL-6, CRP, and TNF-alpha, as well as administered two neuropsychological tests. The results indicate that higher levels of IL-6 were associated with cognitive impairment in this population.
Article
Toxicology
Ha J. Lim, Jae E. Park, Ji S. Han
Summary: This study investigated the effects of (E)-5-hydroxy-7-methoxy-3-(2'-hydroxybenzyl)-4-chromanone on inflammation and hyperglycemia in endotoxin-induced insulin resistant mice. The results showed that (E)-5-hydroxy-7-methoxy-3-(2'-hydroxybenzyl)-4-chromanone effectively regulated blood glucose levels and reduced the phosphorylation of specific proteins in the liver. It also suppressed the activity of nuclear factor-kappa B and activator protein-1, leading to a decrease in the expression of pro-inflammatory cytokines.
TOXICOLOGY RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Wenyu Chen
Summary: In this study, it was found that SNHG7 improves the osteogenic differentiation of hDPSCs by inhibiting miR-6512-3p expression in an inflammatory environment induced by 50 ng/mL TNF-alpha. This provides potential targets for the treatment of periodontitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Jinchao Li, Ying Tang, Ting-Chun Lin, Huawei Zeng, Joel B. Mason, Zhenhua Liu
Summary: Strong evidence suggests an association between body fatness and the development of colorectal cancer (CRC), but there is still limited understanding of the causality between obesity and CRC. This study investigates the role of TNF-alpha in obesity-associated CRC development. The research shows that the deletion of TNF-alpha reduces inflammation and Wnt signaling, thereby attenuating obesity-induced intestinal tumorigenesis. Targeting TNF-alpha signaling may be a potential strategy for reducing the risk of CRC associated with obesity.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2023)
Review
Pharmacology & Pharmacy
Paula Fernanda Kinoshita, Ana Maria Marques Orellana, Vinicius Watanabe Nakao, Natacha Medeiros de Souza Port's, Luis Eduardo Menezes Quintas, Elisa Mitiko Kawamoto, Cristoforo Scavone
Summary: Na+/K+-ATPase is crucial for maintaining the electrochemical gradient and plays a role in regulating calcium signaling and neuronal death. Understanding the interaction between Na+/K+-ATPase and calcium signaling may lead to discovering new targets for treating neurodegenerative diseases.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Endocrinology & Metabolism
Paula C. Bianchi, Lucas Gomes-de-Souza, Willian Costa-Ferreira, Paola Palombo, Paulo E. Carneiro de Oliveira, Sheila A. Engi, Rodrigo M. Leao, Cleopatra S. Planeta, Carlos C. Crestani, Fabio C. Cruz
Summary: The study found that prolonged ethanol vapor exposure increased cardiovascular reactivity to stress in male rats, but had no significant effect on female rats.
BIOLOGY OF SEX DIFFERENCES
(2021)
Review
Immunology
Marina Saade, Giovanna Araujo de Souza, Cristoforo Scavone, Paula Fernanda Kinoshita
Summary: GPNMB is an endogenous glycoprotein that has been recently associated with inflammation and neuroinflammation. It is highly expressed in macrophages and microglia, indicating a potential role in regulating inflammation resolution.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Pharmacology & Pharmacy
Jacqueline Alves Leite, Luiz Henrique Agra Cavalcante-Silva, Martina Raissa Ribeiro, Geovanni de Morais Lima, Cristoforo Scavone, Sandra Rodrigues-Mascarenhas
Summary: This review examines the potential regulatory role of the cardiac steroid ouabain in neutrophilic neuroinflammation.
FRONTIERS IN PHARMACOLOGY
(2022)
Editorial Material
Pharmacology & Pharmacy
Alexis Bailey, Daniel C. Berwick, Rosana Camarini, Cristoforo Scavone
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Review
Pharmacology & Pharmacy
Jacqueline Alves Leite, Elisa Pocas, Gisele Silva Maia, Leandro Barbosa, Luis Eduardo M. Quintas, Elisa Mitiko Kawamoto, Maria Luiza Correia da Silva, Cristoforo Scavone, Luciana E. Drumond de Carvalho
Summary: This article systematically reviews the in vitro effects of cardiotonic steroids on Ca2+ in the brain of rats and mice. The studies demonstrate that ouabain can increase cholinergic, noradrenergic and glutamatergic neurotransmission, significantly increase intracellular signaling molecules such as InsPs, IP3 and cAMP, and stimulate myelin basic protein synthesis. The ouabain-induced intracellular Ca2+ increase may play a crucial role in cellular homeostasis and function.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Vanessa O. Zambelli, Nat Prime Alia Gabriele Hosch, Sarah Farom, Bianca C. Zychar, Diva D. Spadacci-Morena, Luciana Vieira Carvalho, Rui Curi, Lucilia B. Lepsch, Cristoforo Scavone, Osvaldo Augusto Sant'Anna, Luis Roberto C. Goncalves, Yara Cury, Sandra C. Sampaio
Summary: Crotoxin, isolated from the venom of Crotalus durissus terrificus, displays immunomodulatory, anti-inflammatory, and anti-tumoral effects. It promotes leukocyte adherence to endothelial cells and reduces blood cell and lymphocyte numbers. The exact lipoxygenase-derived eicosanoid involved in the immunosuppressive effects of CTX is unknown. This study found that formyl peptide receptors mediate CTX-induced redistribution of lymphocytes and that 15-epi-LXA4 is a key mediator of CTX's immunosuppressive effects.
Article
Multidisciplinary Sciences
Vinicius Wanatable Nakao, Caio Henrique Yokowama Mazucanti, Larissa de Sa Lima, Paloma Segura de Mello, Natacha Medeiros de Souza Port's, Paula Fernanda Kinoshita, Jacqueline Alves Leite, Elisa Mitiko Kawamoto, Cristoforo Scavone
Summary: Alpha-Klotho is an anti-aging protein that has anti-inflammatory and protective effects on renal cells and in the central nervous system. It shows potential in inhibiting neuroinflammation and improving neuronal health.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Paula Fernanda Kinoshita, Ana Maria Orellana, Diana Zukas Andreotti, Giovanna Araujo de Souza, Natalia Prudente de Mello, Larissa Lima, Elisa Mitiko Kawamoto, Cristoforo Scavone
Summary: This study investigated the effects of ouabain on a TNFR1 knockout mouse model. The results showed that the hippocampus of TNFR1 knockout mice exhibited a compensatory mechanism, and ouabain activated TNF-alpha-converting enzyme/adam 17, decreased NR2A expression, and induced anxiety-like behavior in both genotype animals, independent of the presence of TNFR1. However, ouabain only increased IL-1 beta levels in the hippocampus, decreased IL-6 levels in the serum, and increased NR1 expression in wild-type mice, indicating that TNFR1 or TNFR2 expression may be important for some effects of ouabain.
Article
Biochemistry & Molecular Biology
Jacqueline Alves Leite, Ana Maria Orellana, Diana Zukas Andreotti, Amanda Midori Matumoto, Natacha Medeiros de Souza Ports, Larissa de Sa Lima, Elisa Mitiko Kawamoto, Carolina Demarchi Munhoz, Cristoforo Scavone
Summary: This study investigates the effects of endogenous substance ouabain (OUA) on the rat's central nervous system under chronic stress. The results suggest that intermittent administration of OUA can alleviate HPA axis abnormal activity caused by chronic stress, by reducing glucocorticoids levels, CRH-CRHR1 expression, and neuroinflammation via decreasing iNOS activity, without interfering with antioxidant enzymes expression. These changes may lead to the rapid extinction of aversive memory in the hypothalamus and hippocampus.
Article
Multidisciplinary Sciences
Alexandre Chagas Santana, Wellington Andraus, Dan Zimelewicz Oberman, Nicollas Nunes Rabelo, Filipe Miranda Oliveira Silva, Humberto Delle, Rafael Pepineli, Edvaldo Leal de Moraes, Cristoforo Scavone, Larissa de Sa Lima, Sabrina Degaspari, Sergio Brasil, Davi Jorge Fontoura Solla, Liliane Moreira Ruiz, Karina Andrighetti de Oliveira-Braga, Natalia Aparecida Nepomuceno, Paulo Manuel Pego-Fernandes, Stefan Gunther Tullius, Eberval Gadelha Figueiredo
Summary: Liver transplantation is the best treatment for end-stage liver failure, with most livers coming from brain-dead donors. Brain death triggers a broad inflammatory response characterized by increased cytokines, which makes the graft more immunogenic.
SCIENTIFIC REPORTS
(2023)