Journal
NEUROSCIENCE RESEARCH
Volume 70, Issue 1, Pages 118-123Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neures.2011.01.002
Keywords
Mouse hippocampal neuron; Primary culture; Dissociated neuron culture
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Funding
- National Science Council of Taiwan [NSC 98-2311-B-009-001-MY2]
- Ministry of Education Aim for the Top University Plan
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Dissociated hippocampal neuron culture has long been the model system of choice for many neuroscientists. The ability to culture dissociated hippocampal neurons from genetically modified mice provides an invaluable tool for studying many neuronal processes. In this study, we established a novel method to culture dissociated hippocampal neurons from embryonic and neonatal mice. Dissociated neurons were cultured in a microchamber between the glass coverslip and the plastic cell container without the use of glial feeder cells. Our method significantly simplifies the preparation while produces healthy and long-lived neuronal cultures that are difficult to achieve without the use of feeder cells. (C) 2011 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
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