Journal
NEUROSCIENCE RESEARCH
Volume 63, Issue 3, Pages 194-198Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neures.2008.12.003
Keywords
Indoleamine 2; 3-Dioxygenase; IFN-gamma KO mouse; Immunohistochemistry
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Funding
- Grants-in-Aid for Scientific Research [20390167] Funding Source: KAKEN
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Indoleamine 2,3-dioxygenase (IDO), which catabolizes L-tryptophan (L-TRP) to L-kynurenine (L-KYN), is an immunoregulatory factor that is up-regulated via an interferon-gamma (IFN-gamma)-dependent and/or independent mechanism. In this study, we investigated the localization of IDO and whether induction of IDO expression is an IFN-gamma-dependent and/or -independent mechanism in the CNS after cerebral ischemia. The expressions of IDO protein and mRNA were investigated at different time points following cerebral ischemia using immunohistochemistry, immunofluorescence and RT-PCR. Hippocampal neuron IDO mRNA and immunohistochemical staining were significantly up-regulated 72 h after transient global ischemia. Although IFN-gamma is a dominant inducer of IDO, hippocampal neuron IDO was clearly up-regulated in IFN-gamma KO mice. In summary, this is the first finding that up-regulation of IDO in hippocampal neurons after transient global ischemia occurs via INF-gamma-independent mechanisms. (C) 2009 Published by Elsevier Ireland Ltd and the Japan Neuroscience Society.
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