Article
Biochemical Research Methods
Peipei Zhu, Xiaofeng Wu, Ruo-Yu Zhang, Chuan-Chih Hsu, Zhong-Yin Zhang, W. Andy Tao
Summary: Protein phosphatases are important in cellular processes, but studying their substrates and mechanisms is challenging. This study introduces a novel strategy to identify substrates of SHP2 using quantitative proteomics methods, and discovers new substrates.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemical Research Methods
Sergio Alonso-Fernandez, Ignacio Arribas-Diez, Gemma Fernandez-Garcia, Nathaly Gonzalez-Quinonez, Ole N. Jensen, Angel Manteca
Summary: This article describes the expansion of the Streptomyces phosphoproteome using optimized immobilized zirconium (IV) affinity chromatography and mass spectrometry, resulting in the identification of many novel phosphoproteins. The study enhances our understanding of Streptomyces development and secondary metabolism regulation.
JOURNAL OF PROTEOMICS
(2022)
Article
Nanoscience & Nanotechnology
Danqing Wang, Junfeng Huang, Haoran Zhang, Ting-Jia Gu, Lingjun Li
Summary: Protein phosphorylation is an important PTM involved in many cellular functions. This study developed a novel platform for phosphopeptide enrichment using a phosphorylated cotton-fiber-based Ti(IV)-IMAC material. The material showed high efficiency and selectivity in enriching phosphopeptides and demonstrated excellent sensitivity and specificity in complex biological samples.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Gugu Hao, Rong Hu, Xiaoqin Wang, Pan Gao, Lin Wang, Manhua Jiang, Libo Xin, Guoying Tan, Yanping Zhao, Fuzhou Sun, Dexin Chu, Jinqian Lv, Jinsong You, Fangfang Huang, Xuezhi Song
Summary: In recent years, the presence of nitrosamines in drug products has become a regulatory concern. This manuscript provides an industry perspective on nitrosamines research with a focus on Nnitrosodimethylamine (NDMA) in metformin hydrochloride sustained-release tablets (MET). The manuscript identifies the key factors contributing to NDMA formation in MET and proposes strategies to mitigate the risk of nitrosamine formation in finished drug products, such as avoiding excipients with nitrite, nitrate, and peroxide impurities, and maintaining appropriate formulation conditions.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Review
Chemistry, Analytical
Khaja Moinuddin Shaik, Bhaskar Sarmah, Gaurav Suresh Wadekar, Pramod Kumar
Summary: This review discusses the recent detection of nitrosamine impurities in pharmaceutical products, including various detection methods, mechanisms of action, sample preparation techniques, and regulatory limits.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Mi Luo, Liyun Zheng, Suwei Wang, Jianghua Zhu, Zhuohong Tang, Pingming Gao
Summary: A 2D LC-QTOF/MS/MS method was developed to characterize four major degradation products of metformin under different conditions, with two impurities never reported before identified for the first time in this study.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Plant Sciences
Wasinee Pongprayoon, Atikorn Panya, Janthima Jaresitthikunchai, Narumon Phaonakrop, Sittiruk Roytrakul
Summary: This study aims to identify novel chitosan-responsive phosphoproteins in drought-sensitive rice cultivars LPT123 and KDML105 and compare their responses to chitosan treatment. The results showed that 22 proteins had different expression levels in response to chitosan treatment before osmotic stress.
Review
Biochemistry & Molecular Biology
Olga Shevchuk, Antonija Jurak Begonja, Stepan Gambaryan, Matthias Totzeck, Tienush Rassaf, Tobias B. Huber, Andreas Greinacher, Thomas Renne, Albert Sickmann
Summary: This review discusses the role of proteomics in understanding the molecular basics of traditional and emerging functions of platelets, as well as the application of proteomic technologies in platelet biogenesis, signaling, and storage. Additionally, the potential of newly appeared trapped ion mobility spectrometry (TIMS) in platelet research is highlighted, along with discussions on the implementation of proteomic methods in platelet transfusion medicine, diagnostics, and prognostics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Shereen M. Aleidi, Lina A. Dahabiyeh, Xinyun Gu, Mohammed Al Dubayee, Awad Alshahrani, Hicham Benabdelkamel, Muhammad Mujammami, Liang Li, Ahmad Aljada, Anas M. Abdel Rahman
Summary: Metformin has metabolic effects in T2DM and obesity, with 30 dysregulated metabolites in obese diabetic patients restored to control levels by metformin. Interestingly, 9 metabolites were significantly dysregulated between lean and obese cohorts due to T2DM and metformin regardless of obesity status, providing insight into potential molecular targets of metformin in T2DM and obesity.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Tigran Margaryan, Mackenna Elliott, Nader Sanai, Artak Tovmasyan
Summary: A sensitive and rapid LC-MS/MS method was developed for quantifying LY3214996, abemaciclib, and their metabolites in human samples. The method was successfully applied in a clinical trial.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2022)
Article
Chemistry, Analytical
Xuefei Zhong, Shruti Nayak, Lili Guo, Shivkumar Raidas, Yunlong Zhao, Rachel Weiss, Matthew Andisik, Chinnasamy Elango, Giane Sumner, Susan C. Irvin, Michael A. Partridge, Hong Yan, Sook E. Yen, Haibo Qiu, Yuan Mao, Albert Torri, Ning Li
Summary: REGEN-COV, a cocktail of two human monoclonal antibodies, has shown promising results in reducing SARS-CoV-2 viral load in COVID-19 patients. A liquid chromatography-mass spectrometry method was developed for the determination of REGEN-COV concentrations in serum samples from patients participating in clinical trials. The concentrations measured by this method were comparable to those measured by a validated immunoassay.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Berkley Luck, Thomas D. Horvath, Kristen A. Engevik, Wenly Ruan, Sigmund J. Haidacher, Kathleen M. Hoch, Numan Oezguen, Jennifer K. Spinler, Anthony M. Haag, James Versalovic, Melinda A. Engevik
Summary: The study revealed that Bifidobacterium dentium has the capacity to produce the important neurotransmitter gamma-aminobutyric acid (GABA) and can impact neurotransmitter concentrations in vivo, indicating a potential role of B. dentium in neurotransmitter regulation.
Article
Biochemical Research Methods
Christine Wegler, Aljona Saleh, Annika Lindqvist, Hedvig Nordeng, John Smeraglia, Pawel Baranczewski
Summary: The majority of women have health problems that require medication after giving birth. However, there is limited information on the transfer of drugs to breast milk and their potential effects on infants. Therefore, more research needs to be conducted in larger populations. Researchers have developed two rapid and sensitive analysis methods for quantifying drugs in human breast milk, and have validated the accuracy and precision of these methods.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Chemistry, Applied
A. Rego, I. Coelho, C. Motta, C. Cardoso, A. Gomes-Bispo, C. Afonso, N. M. Bandarra, J. A. L. Silva, I. Castanheira, J. A. M. Prates
Summary: Chub mackerel is an underutilised fish species that contains high levels of selenium and vitamin B12, which are beneficial for preventing cognitive decline and dementia. The content of these nutrients varies seasonally, emphasizing the importance of a year-round assessment to provide accurate dietary recommendations.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Biochemistry & Molecular Biology
Elisa Diaz-Riera, Maisa Garcia-Arguinzonis, Laura Lopez, Xavier Garcia-Moll, Lina Badimon, Teresa Padro
Summary: This study used untargeted mass spectrometry (MS) proteomic approach to identify differential urine protein signature in acute decompensated heart failure (ADHF) patients and examine its pathophysiological link to disease evolution. The study found a pattern of 26 differential proteins, mostly of hepatic origin, in the urine samples of ADHF patients. The study also identified the top four biological pathways associated with the ADHF proteome, including retinol metabolism and transport, immune response/inflammation, extracellular matrix organization, and platelet degranulation. The protein transthyretin (TTR) was widely represented among these pathways. Quantitative analysis validated the proteomic results and showed that combining urine levels of TTR and retinol-binding protein 4 (RBP4) can effectively discriminate ADHF patients with renal dysfunction and predict poor disease evolution over an 18-month follow-up.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
R. Khang, C. Park, J. -H. Shin
Article
Geriatrics & Gerontology
Yun-Il Lee, Hojin Kang, Young Wan Ha, Ki-Young Chang, Sung-Chun Cho, Sang Ok Song, Hyein Kim, Areum Jo, Rin Khang, Jeong-Yun Choi, Yunjong Lee, Sang Chul Park, Joo-Ho Shin
NEUROBIOLOGY OF AGING
(2016)
Article
Oncology
Hojin Kang, Rin Khang, Sangwoo Ham, Ga Ram Jeong, Hyojung Kim, Minkyung Jo, Byoung Dae Lee, Yun Il Lee, Areum Jo, ChiHu Park, Hyein Kim, Jeongkon Seo, Sun Ha Paek, Yun-Song Lee, Jeong-Yun Choi, Yunjong Lee, Joo-Ho Shin
Article
Biochemistry & Molecular Biology
Hojin Kang, Areum Jo, Hyein Kim, Rin Khang, Ji-Yeong Lee, Hanna Kim, Chi-Hu Park, Jeong-Yun Choi, Yunjong Lee, Joo-Ho Shin
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2018)
Article
Biochemistry & Molecular Biology
Ji-Yeong Lee, Hanna Kim, Areum Jo, Rin Khang, Chi-Hu Park, Soo-Jeong Park, Eunsang Kwag, Joo-Ho Shin
Summary: The interaction between alpha-syn A53T and TADA2a may lead to changes in histone modification, which are associated with neurotoxicity in the pathology of PD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Areum Jo, Yunjong Lee, Tae-In Kam, Sung-Ung Kang, Stewart Neifert, Senthilkumar S. Karuppagounder, Rin Khang, Hojin Kang, Hyejin Park, Shih-Ching Chou, Sungtaek Oh, Haisong Jiang, Deborah A. Swing, Sangwoo Ham, Sheila Pirooznia, George K. E. Umanah, Xiaobo Mao, Manoj Kumar, Han Seok Ko, Ho Chul Kang, Byoung Dae Lee, Yun-Il Lee, Shaida A. Andrabi, Chi-Hu Park, Ji-Yeong Lee, Hanna Kim, Hyein Kim, Hyojung Kim, Jin Whan Cho, Sun Ha Paek, Chan Hyun Na, Lino Tessarollo, Valina L. Dawson, Ted M. Dawson, Joo-Ho Shin
Summary: Accumulation of parkin-interacting substrate (PARIS) due to inactivation of parkin contributes to Parkinson's disease (PD) through repression of peroxisome proliferator-activated receptor-gamma coactivator-1 alpha (PGC-1 alpha) activity. Farnesol, identified as an inhibitor of PARIS, promotes farnesylation of PARIS and prevents its repression of PGC-1 alpha by reducing PARIS occupancy on the PPARGC1A promoter. Studies show that farnesol can prevent dopaminergic neuronal loss and behavioral deficits by enhancing PARIS farnesylation, suggesting a potential therapeutic role in PD treatment.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Rin Khang, Areum Jo, Hojin Kang, Hanna Kim, Eunsang Kwag, Ji-Yeong Lee, Okjae Koo, Jinsu Park, Hark Kyun Kim, Dong-Gyu Jo, Inwoo Hwang, Jee-Yin Ahn, Yunjong Lee, Jeong-Yun Choi, Yun-Song Lee, Joo-Ho Shin
Summary: Although the physiological functions of most K-ZNF proteins are still unknown, the Zfp212 protein, highly conserved in mammals and abundant in the brain, plays an important role in Purkinje cell survival through its relationship with genes such as Pld3.
SCIENTIFIC REPORTS
(2021)
Article
Ophthalmology
Nelson Chen, Hane Lee, Angela H. Kim, Pei-Kang Liu, Eugene Yu-Chuan Kang, Yun-Ju Tseng, Go Hun Seo, Rin Khang, Laura Liu, Kuan-Jen Chen, We-Chi Wu, Meng-Chang Hsiao, Nan-Kai Wang
Summary: This case report presents a novel homozygous variant in the PCDH15 gene in a patient with USH1F, leading to congenital hearing loss and syndromic RP. The identified pathogenic variant is expected to result in NMD of PCDH15 mRNA.
Article
Biochemistry & Molecular Biology
Hyein Kim, Hojin Kang, Yunjong Lee, Chi-Hu Park, Areum Jo, Rin Khang, Joo-Ho Shin
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2017)
Article
Neurosciences
Song Xue, Feng Kong, Yiying Song, Jia Liu
Summary: This study used resting-state functional magnetic resonance imaging to explore the relationship between individual's spontaneous neural activity and social interaction anxiety in a nonclinical population. The results showed that social interaction anxiety was correlated with the fractional amplitude of low-frequency fluctuations in several brain regions, and that emotional intelligence partially mediated this relationship. This study provides evidence for the neural basis of social interaction anxiety in the normal population and highlights the role of emotional intelligence in this anxiety.
NEUROSCIENCE LETTERS
(2024)
Article
Neurosciences
Katsuyuki Yamaguchi, Takuya Yazawa
Summary: This study provides morphometric data on the development of the human medullary arcuate nucleus (AN) by examining the brains of preterm and perinatal infants. The results show that AN morphology demonstrates asymmetry and individual variability during the fetal period. The volume and neuronal number of AN increase exponentially with age, while neuronal density decreases exponentially. The AN may undergo neuron death and neuroblasts production after mid-gestation.
NEUROSCIENCE LETTERS
(2024)
Article
Neurosciences
Zhan Zhou, Weixin Dai, Tianxiao Liu, Min Shi, Yi Wei, Lifei Chen, Yubo Xie
Summary: Studies have shown that propofol-induced neurotoxicity is caused by disruption of mitochondrial fission and fusion, leading to an energy supply imbalance for developing neurons. Healthy mitochondria released by astrocytes can migrate to compromised neurons to mitigate propofol-induced neurotoxicity, but the exact mechanisms involved still need further clarification.
NEUROSCIENCE LETTERS
(2024)
Article
Neurosciences
An Chen, Song Hao, Yongpeng Han, Yang Fang, Yibei Miao
Summary: This study explores the efficacy of two forms of BCI attention training games and finds that physical games may be more effective than video games. The research also offers valuable insights for future game design from a neuroscience perspective.
NEUROSCIENCE LETTERS
(2024)
Article
Neurosciences
Lina Liu, Luran Liu, Yunting Lu, Tianyuan Zhang, Wenting Zhao
Summary: This study reveals that GDI1 serves as a potential diagnostic biomarker for AD and inhibition of GDI1 can attenuate Aβ-induced neurotoxicity. The findings offer new insights for the treatment of AD.
NEUROSCIENCE LETTERS
(2024)
Article
Neurosciences
Zahra Gholami, Ava Soltani Hekmat, Ali Abbasi, Kazem Javanmardi
Summary: This study investigated the effects of alamandine on allodynia in a rat model and found the presence of MrgD receptors in the vlPAG and RVM regions. Microinjection of alamandine resulted in a significant increase in paw withdrawal threshold and could be blocked by an MrgD receptor antagonist. Upregulation of MrgD receptor expression following allodynia induction suggests a potential compensatory mechanism in response to pain.
NEUROSCIENCE LETTERS
(2024)
Article
Neurosciences
Mingliang Xu, Lei Xia, Junjie Li, Yehong Du, Zhifang Dong
Summary: This study found that DHF effectively alleviates sevoflurane-induced cognitive impairment in developing mice by restoring the balance between tau O-GlcNAcylation and phosphorylation. Therefore, DHF has the potential to be a therapeutic agent for treating cognitive impairment associated with anesthetics, such as sevoflurane.
NEUROSCIENCE LETTERS
(2024)
Article
Neurosciences
Tsubasa Mitsutake, Hisato Nakazono, Takanori Taniguchi, Hisayoshi Yoshizuka, Maiko Sakamoto
Summary: The posterior parietal cortex plays a crucial role in postural stability, and transcranial electrical stimulation of this region can modulate physical control responses. This study found that cathodal stimulation significantly decreased joint angular velocity in multiple directions, while there were no significant differences with transcranial random noise stimulation.
NEUROSCIENCE LETTERS
(2024)
Article
Neurosciences
Xishuai Yang, Wei Zhang, Xueli Chang, Zuopeng Li, Runquan Du, Junhong Guo
Summary: This study aims to evaluate the efficacy of low-dose rituximab (RTX) in patients with muscle-specific kinase antibody positive myasthenia gravis (MuSK-MG). The results showed that low-dose RTX treatment led to significant improvements in clinical symptoms and quality of life for patients with MuSK-MG.
NEUROSCIENCE LETTERS
(2024)
Article
Neurosciences
Jian Zhang, Shunyuan Guo, Rong Tao, Fan Wang, Yihong Xie, Huizi Wang, Lan Ding, Yuejian Shen, Xiaoli Zhou, Junli Feng, Qing Shen
Summary: This study established an Alzheimer's disease (AD) model of zebrafish induced by AlCl3 and found that marine-derived plasmalogens (Pls) could alleviate cognitive impairments of AD zebrafish by reversing athletic impairment and altering the expression levels of genes related to oxidative stress, ferroptosis, synaptic dysfunction, and apoptosis.
NEUROSCIENCE LETTERS
(2024)
Article
Neurosciences
Lu Li, Jiaqi Ren, Qi Fang, Liqiang Yu, Jintao Wang
Summary: ICU-AW is a common and severe neuromuscular complication in critically ill patients. Electrophysiological examination is essential for accurate diagnosis and early prediction of the disease. This study aimed to establish and validate an ICU-AW predictive model in SIRS patients, providing a practical tool for early clinical prediction.
NEUROSCIENCE LETTERS
(2024)
Article
Neurosciences
Ahmad Alipour, Roghayeh Mohammadi
Summary: The present study aimed to investigate the separate and combined effects of anodal transcranial direct current stimulation (tDCS) over the primary motor cortex (M1) and left dorsolateral prefrontal cortex (F3) regions on pain relief in patients with type-2 diabetes suffering from neuropathic pain (NP). The results showed that tDCS had the potential to induce pain relief in patients with type-2 diabetes suffering from NP. The mean perceived pain intensity in the posttest was lower in the M1 stimulation group than in the F3 stimulation group. However, more trials with larger sample sizes are necessary to define clinically relevant effects.
NEUROSCIENCE LETTERS
(2024)
Article
Neurosciences
Eduardo J. Fusse, Franciele F. Scarante, Maria A. Vicente, Mariana M. Marrubia, Flavia Turcato, Davi S. Scomparin, Melissa A. Ribeiro, Maria J. Figueiredo, Tamires A. V. Brigante, Francisco S. Guimaraes, Alline C. Campos
Summary: Repeated exposure to psychosocial stress alters the endocannabinoid system and affects brain regions associated with emotional distress. Enhancing the effects of endocannabinoids through pharmacological inhibition induces an anti-stress behavioral effect, possibly mediated by the mTOR signaling pathway.
NEUROSCIENCE LETTERS
(2024)
Article
Neurosciences
Giulia Agostoni, Luca Bischetti, Federica Repaci, Margherita Bechi, Marco Spangaro, Irene Ceccato, Elena Cavallini, Luca Fiorentino, Francesca Martini, Jacopo Sapienza, Mariachiara Buonocore, Michele Francesco D'Incalci, Federica Cocchi, Carmelo Guglielmino, Roberto Cavallaro, Marta Bosia, Valentina Bambini
Summary: This study found a general impairment in humor comprehension in individuals with schizophrenia, with mental jokes being more difficult for both patients and controls. Humor comprehension was closely associated with the patients' overall pragmatic and linguistic profile, while the association with Theory of Mind (ToM) was minimal. Another notable finding was the increased appreciation of humor in individuals with schizophrenia, who rated jokes as funnier than controls did, regardless of whether they were correctly or incorrectly completed. The funniness ratings were not predicted by any measure, suggesting a dimension of humor untied to cognition or psychopathology.
NEUROSCIENCE LETTERS
(2024)
Article
Neurosciences
Xiuping Gong, Qi Li, Yang Liu
Summary: This study demonstrates that Sev targets CREBBP to inhibit ALG13 transcription, leading to hippocampal damage and cognitive impairment.
NEUROSCIENCE LETTERS
(2024)
