Journal
NEUROSCIENCE LETTERS
Volume 504, Issue 3, Pages 265-270Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2011.09.044
Keywords
Anxiety; Two-way avoidance acquisition; cDNA microarray; RT-PCR; Gene expression; Roman rats
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Funding
- Junta de Andalucia [BIO-302]
- Ministerio de Ciencia y Tecnologia, Spanish [SEJ2004-03231/PSIC, PSI2010-15787, PSI2009-10532]
- Fundacio la MARATO TV3 [092630/31]
- DGR [2009 SGR-51]
- EURATRANS European project [HEALTH-F4-2010-241504]
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Microarray technology was used to explore differences in brain gene expression under basal conditions in two strains of psychogenetically selected rats which differ in anxiety/stress responses, the inbred Roman High-(RHA-I) and Roman Low-(RLA-I) Avoidance rats. Microarray analysis detected 14 up-regulated and 24 down-regulated genes in RLA-I vs. RHA-I rats functionally related to neurobiological processes. The differentially expressed genes CAMKK2, CRHBP, EPHX2, HOMER3, NDN, PRL and RPL6 were selected for microarray validation using qRT-PCR. EPHX2, CAMKK2 (both up-regulated in RLA-I vs. RHA-I rats) and HOMER3 (down-regulated in RLA-I vs. RHA-I rats) showed a similar tendency and fold-change both in microarray and RT-PCR analyses: PRL (up-regulated in RLA-I vs. RHA-I rats), CRHBP and RPL6 (both down-regulated in RLA-I vs. RHA-I animals) showed a similar tendency but a different order of magnitude of change among experiments: finally, NDN was validated neither in tendency nor in magnitude of change. Published by Elsevier Ireland Ltd.
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