Journal
NEUROSCIENCE LETTERS
Volume 473, Issue 2, Pages 107-109Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2010.02.029
Keywords
Curcumin; Muscarinic M-1 cholinoceptors; Radioligand binding; Uninary bladder; Wistar rat
Categories
Funding
- Food Safety Commission, Japan [0807]
Ask authors/readers for more resources
Curcumin is an active principle contained in rhizome of Curcuma longa, and it has been recently mentioned to show affinity to muscarinic M-1 cholinoceptors (M-1-mAChR). In the present study, we found that curcumin caused a concentration-dependent increase of muscle tone in urinary bladder isolated from Wistar rats. This action was inhibited by pirenzepine at concentration enough to block M-1-mAChR. In radioligand-binding assay, specific binding of [H-3]-oxotremorine (OXO-M) in the rat bladder homogenates was also displaced by curcumin in a concentration-dependent manner. In the presence of inhibitors for PLC-PKC pathway, either U73122 (phospholipase C inhibitor) or chelerythrine (protein kinase C inhibitor), curcumin-stimulated contraction in urinary bladder was markedly reduced. In conclusion, the obtained results suggest that curcumin can activate M-1-mAChR at concentrations lower than to scavenge free radicals to increase of muscle tone in urinary bladder through PLC-PKC pathway. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available