Journal
NEUROSCIENCE LETTERS
Volume 440, Issue 2, Pages 170-175Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2008.05.045
Keywords
neurite outgrowth; RhoA; small hairpin RNA; RNA interference; PC12 cells
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In order to promote neurite outgrowth, we constructed a plasmid producing RhoA-specific small hairpin RNA (shRNA) to block RhoA expression and tested its actions in PC12 cells. Our results show that the shRNA-mediated RNA interference (RNAi) successfully suppressed RhoA expression. As a consequence of RhoA knockdown, F-actin levels were significantly reduced and processes were markedly induced. These processes express two neurite markers, neurofilament and Pill tubulin. This study demonstrates that plasmid-producing shRNA specific for RhoA can act as an effective tool to induce neurite outgrowth and further confirms the neurite growth-inhibitory role of RhoA. This shRNA may thus be a useful tool in promoting neurite outgrowth and could be applicable in neural repair after CNS injury. (c) 2008 Elsevier Ireland Ltd. All rights reserved.
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