Journal
NEUROSCIENCE LETTERS
Volume 442, Issue 1, Pages 63-68Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2008.05.119
Keywords
huntingtin; polyglutamine; mouse neuroblastoma cell; neurite outgrowth; cell death; apoptosis
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Funding
- National Natural Science Foundation of China [30225024]
- Taishan Scholar construction Projected Shandong Province
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Huntington's disease (HD) is an autosomal dominant inheritable neurodegenerative disorder caused by expansion of a polyglutamine repeat in the amino-terminal region of huntingtin. Polyglutamine expansion causes mutant huntingtin to aggregate and accumulate in the nuclei and cytoplasm of neurons. The aggregated amino-terminal fragments of mutant huntingtin are toxic to neuronal cells and may be involved in the neurodegeneration in HD patient brains. Although nuclear mutant huntingtin has been found to affect gene expression, the effect of cytoplasmic mutant huntingtin remains to be investigated. We established stably transfected mouse neuroblastoma (N2a) cells that express soluble amino-terminal fragments of huntingtin containing 20 (20Q) or 150 (150Q) glutamine repeats. in these stable cell lines, both 20Q and 150Q are diffusely distributed in the cytoplasm without aggregate formation. However, the stable 150Q cells are deficient in neurite outgrowth. Compared with wild-type N2a cells and cells stably expressing 200, stable 150Q cells also have decreased viability and are more susceptible to apoptotic stimulation. These findings suggest that the cytoplasmic soluble mutant huntingtin is also toxic to cells. (c) 2008 Published by Elsevier Ireland Ltd.
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