Article
Chemistry, Applied
Wen Zeng, Hua Hui, Zhongyang Liu, Zhen Chang, Mingbo Wang, Baorong He, Dingjun Hao
Summary: NGF-loaded chitosan/PLGA composite microspheres ionically cross-linked by tripolyphosphate (TPP/Chitosan/PLGA-NGF) were successfully prepared to control NGF release in injured peripheral nerves. Analysis of encapsulation efficiency, size, stability, and release rate confirmed the effectiveness of TPP/Chitosan/PLGA-NGF microspheres for neural tissue repair in both in vitro and in vivo experiments.
CARBOHYDRATE POLYMERS
(2021)
Review
Biochemistry & Molecular Biology
Trevor Poitras, Douglas W. Zochodne
Summary: Common mechanisms of peripheral axon regeneration are recruited following diverse forms of damage to peripheral nerve axons. Supporting peripheral axon regrowth could be achieved through direct axon support or manipulation of the inflammatory milieu. Understanding the molecular barriers to regrowth and exploiting the downstream signaling pathways that impact neuron growth are promising approaches for promoting adult peripheral axon regeneration. This review emphasizes intrinsic aspects of adult peripheral axon regeneration and highlights several molecular barriers to regrowth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Yuting Cai, Qun Huang, Penghui Wang, Kaichuang Ye, Zhen Zhao, Haomin Chen, Zhenjing Liu, Hongwei Liu, Hoilun Wong, Mohsen Tamtaji, Kenan Zhang, Feng Xu, Guorui Jin, Lun Zeng, Jianbo Xie, Yucong Du, Zhigang Hu, Dazhi Sun, Jinbao Qin, Xinwu Lu, Zhengtang Luo
Summary: This study develops a graphene-based nerve guidance conduit for diabetic patients with peripheral nerve injury. The conduit incorporates a natural double network hydrogel and a neurotrophic concentration gradient, providing good biocompatibility and sustained growth factor release. The graphene mesh improves the toughness and conductivity of the conduit, promoting regeneration.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Chemistry, Applied
Sergey O. Solomevich, Carlo M. Oranges, Daniel F. Kalbermatten, Anna Schwendeman, Srinivas Madduri
Summary: Peripheral nerve repair is a serious problem in neurosurgery, with poor clinical outcomes and significant socioeconomic burden. Biodegradable polysaccharides show great potential for improving nerve regeneration. This review focuses on the promising therapeutic strategies involving different types of polysaccharides and their composites for promoting nerve regeneration, highlighting their applications in nerve guidance conduits, hydrogels, nanofibers, and films. The ease of therapeutic implementation, drug release properties, therapeutic outcomes, and future research directions are also discussed.
CARBOHYDRATE POLYMERS
(2023)
Article
Engineering, Multidisciplinary
Haixing Xu, Yi Yu, Lingxi Zhang, Furong Zheng, Yixia Yin, Yuxiang Gao, Kebi Li, Jingyi Xu, Jing Wen, Hui Chen, Zhixin Lei, Zhijun Huang, Dan Yu, Nan Jiang, Peihu Xu
Summary: The study investigates the repair of injured peripheral nerves in rats using PDLLA/beta-TCP nerve conduits laden with CS-HA/NGF hydrogels, showing that the hydrogels promote adhesion, spreading, and differentiation of neuronal cells while sustaining NGF release. In vivo studies demonstrate a significant positive effect on axon regeneration and myelination compared to control groups, indicating the potential of CS-HA/NGF injectable hydrogel in promoting nerve regeneration in neural tissue engineering.
COMPOSITES PART B-ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Wanlin Xu, Zhen Zhang, Hao Lu, Yifan Wu, Jia Liu, Shengwen Liu, Wenjun Yang
Summary: The study created a PU/Gel/VEGF conduit which promotes proliferation, migration, and expression of neural markers in nerve cells, aiding in functional recovery of the injured side.
MACROMOLECULAR BIOSCIENCE
(2022)
Review
Biotechnology & Applied Microbiology
Ane Escobar, Rui Luis Reis, Joaquim Miguel Oliveira
Summary: Peripheral nerve injuries are a common cause of disabilities, and treatment with long nerve gap autografts is currently considered the best method. However, due to limited availability and potential issues at the donor site, research is focusing on developing regenerative strategies using artificial nerve guidance conduits (NGCs). These NGCs can be optimized through various methods to enhance nerve regeneration, and recent advancements in using different types of nanoparticles to introduce growth factors to NGCs are discussed in depth.
Article
Biochemistry & Molecular Biology
Poornima Ramburrun, Pradeep Kumar, Elias Ndobe, Yahya E. Choonara
Summary: This study evaluated a biosimulated nerve repair system providing three types of regenerative cues in a peripheral nerve injury model. The nanofibers supported PC12 proliferation, preserved muscle mass, and gradually improved functional recovery. The combination of physical guidance, chemical cues, and therapeutic cues offers a promising strategy for the regeneration of severed peripheral nerves.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Yi-jun Liu, Xiao-feng Chen, Li-ping Zhou, Feng Rao, Dian-ying Zhang, Yan-hua Wang
Summary: This study aimed to analyze the effect and mechanism of Wnt5a-loaded fibrin hydrogel on a 10-mm rat sciatic nerve defect. Results showed that Wnt5a could promote Schwann cell proliferation and the expression of various neurotrophic factors, and the Wnt5a/Fn group outperformed the control groups in terms of nerve regeneration and functional recovery 12 weeks after the operation. The Wnt5a/Fn nerve conduit may promote peripheral nerve defect regeneration by facilitating multiple neurotrophin secretion, combining vascularization and neurotrophic growth cues.
CNS NEUROSCIENCE & THERAPEUTICS
(2022)
Article
Veterinary Sciences
M. Krautmann, R. Walters, P. Cole, J. Tena, L. M. Bergeron, J. Messamore, D. Mwangi, S. Rai, P. Dominowski, K. Saad, Y. Zhu, M. Guillot, L. Chouinard
Summary: The experimental results demonstrate that Bedinvetmab has good safety in laboratory Beagle dogs, with no treatment-related adverse changes observed in clinical evaluations, joints, immune morphology or function. Short-term concurrent use of nonsteroidal anti-inflammatory drugs also had no significant effects.
VETERINARY JOURNAL
(2021)
Review
Biochemistry & Molecular Biology
Andrea Lavorato, Stefania Raimondo, Marina Boido, Luisa Muratori, Giorgia Durante, Fabio Cofano, Francesca Vincitorio, Salvatore Petrone, Paolo Titolo, Fulvio Tartara, Alessandro Vercelli, Diego Garbossa
Summary: The study discusses the features and applications of different types of mesenchymal stem cells in peripheral nerve regeneration after nerve injury. ADSCs are considered the most suitable MSCs for recovery from peripheral nerve lesions due to their excellent support and stimulation of axonal growth, remarkable paracrine activity, differentiation potential, low immunogenicity, and high survival rate.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Xiandong Zhu, Shangjing Xie, Jiawei Chen, Qiaohong Lu, Xiaowu Wang, Feixiang Duan, Sinian Xu, Yan Zhang, Hongjian Huang, Yongqiang Wang, Hongwei Wang, Bicheng Chen, Huanjie Huang
Summary: This study investigates the potential mechanism underlying the therapeutic effect of sildenafil in combination with islet transplantation for diabetic peripheral neuropathy. The results suggest that islet transplantation can reverse sciatic nerve injury in diabetic mice, and sildenafil enhances the therapeutic effect of islet transplantation.
INTERNATIONAL JOURNAL OF ENDOCRINOLOGY
(2023)
Article
Cell Biology
Daniel Hellenbrand, Clayton L. Haldeman, Jae-Sung Lee, Angela G. Gableman, Elena K. Dai, Stephen D. Ortmann, Jerrod C. Gotchy, Kierra K. Miller, Adrianna M. Doucas, Nicole C. Nowak, William L. Murphy, Amgad S. Hanna
Summary: The gold standard for treating peripheral nerve injuries with large nerve gaps is autologous nerve grafts. However, incorporating mineral coated microparticles (MCMs) to release growth factors like NGF and GDNF can significantly enhance axon growth and improve limb function in rats with nerve grafts.
NEURAL REGENERATION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Yuan Tian, Tomohiro Onodera, Mohamad Alaa Terkawi, Koji Iwasaki, Ryosuke Hishimura, Dawei Liang, Takuji Miyazaki, Norimasa Iwasaki
Summary: The study found that low-dose NGF antibody injections can alleviate pain in rats with OA, while local treatment does not worsen OA progression. Local administration may serve as an alternative treatment for patients with OA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Lorena R. Lizarraga-Valderrama, Giulia Ronchi, Rinat Nigmatullin, Federica Fregnan, Pooja Basnett, Alexandra Paxinou, Stefano Geuna, Ipsita Roy
Summary: The study demonstrated that PHA-NGCs are highly promising in promoting peripheral nerve regeneration, showing comparable outcomes to autografts in functional evaluation and nerve morphology.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
John Marcek, Carlin Okerberg, Chang-Ning Liu, David Potter, Paul Butler, Magalie Boucher, Mark Zorbas, Peter Mouton, Jens R. Nyengaard, Chris Somps
JOURNAL OF CHEMICAL NEUROANATOMY
(2016)
Article
Pharmacology & Pharmacy
Matthew R. Zahner, Chang-Ning Liu, Carlin V. Okerberg, Alan C. Opsahl, Walter F. Bobrowski, Chris J. Somps
JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS
(2016)
Article
Toxicology
Chang-Ning Liu, Edwin Berryman, David Zakur, Ahmed M. Shoieb, Ingrid D. Pardo, Magalie Boucher, Chris J. Somps, Chedo M. Bagi, Jon C. Cook
JOURNAL OF APPLIED TOXICOLOGY
(2018)
Article
Pharmacology & Pharmacy
Matthew R. Zahner, Chang-Ning Liu, Vincent Bernardo, Carrie Northcott, Cheryl Tyszkiewicz, Carlin Okerberg, Magalie Boucher, Ingrid Pardo, Chris J. Somps
JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS
(2017)
Article
Ophthalmology
Chang-Ning Liu, Betty Pettersen, Gary Seitis, Sarah Osgood, Chris Somps
CUTANEOUS AND OCULAR TOXICOLOGY
(2014)
Article
Toxicology
Chang-Ning Liu, Nagappan Mathialagan, Patrick Lappin, Jay Fortner, Chris Somps, Gary Seitis, Theodore R. Johnson, Wenyue Hu, Diane Matsumoto
TOXICOLOGICAL SCIENCES
(2015)
Article
Multidisciplinary Sciences
Qinghai Peng, Jordan Mechanic, Ahmed Shoieb, Ingrid D. Pardo, Laura Schaevitz, Judith Fenyk-Melody, Allison Vitsky, Magalie Boucher, Chris Somps, Jon C. Cook, Chang-Ning Liu
Article
Pharmacology & Pharmacy
Chang-Ning Liu, Jeffrey Morin, Melba Dokmanovich, Crystal T. Bluette, Richard Goldstein, Balasubramanian Manickam, Cedo M. Bagi
JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS
(2019)
Article
Pathology
Karen M. Walters, Magalie Boucher, Germaine G. Boucher, Alan C. Opsahl, Peter R. Mouton, Chang-Ning Liu, Casey R. Ritenour, Thomas T. Kawabe, Hayley N. Pryski, Christopher J. Somps
TOXICOLOGIC PATHOLOGY
(2020)
Retraction
Veterinary Sciences
Xian Chen, Chang-Ning Liu, Judith E. Fenyk-Melody
JOURNAL OF THE AMERICAN ASSOCIATION FOR LABORATORY ANIMAL SCIENCE
(2019)
Article
Multidisciplinary Sciences
Caroline B. Winn, Seo-Kyoung Hwang, Jeffrey Morin, Crystal T. Bluette, Balasubramanian Manickam, Ziyue K. Jiang, Anand Giddabasappa, Chang-Ning Liu, Kristin Matthews
Summary: This study aimed to identify objective non-invasive criteria for predicting imminent distress and mortality in metastatic lung tumor-bearing mice. The findings suggest that increases in respiratory rate can serve as an objective benchmark to signal the need for increased clinical observations or euthanasia. Adoption of this novel humane endpoint criterion would allow investigators time to collect tissue samples prior to spontaneous morbidity or death and significantly reduce the distress of mice in the terminal stages of these metastatic lung tumor models.
Article
Pharmacology & Pharmacy
Seo-Kyoung Hwang, Cheryl Tyszkiewicz, Jeffrey Morin, Gary R. Point, Chang-Ning Liu
Summary: A new in- and ex vivo hybrid method for measuring GFR using the in vivo imaging system (IVIS) was developed as an alternative to the traditional filtration marker method with fluorescence molecular tomography (FMT). The results showed that the IVIS imaging measurement of inulin clearance in untreated, vehicle-treated and cyclosporine A-treated mice yielded comparable GFR values to those obtained with FMT imaging, demonstrating the feasibility and reliability of this alternative technique for assessing renal function in pharmacological and toxicological studies.
JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS
(2021)
Article
Multidisciplinary Sciences
Seo-Kyoung Hwang, Cheryl Tyszkiewicz, Melissa Dragon, Kimberly Navetta, Rebecca Ferreira, Chang-Ning Liu
Summary: This study found that the introduction of a gloved hand into a cage can trigger adult male albino rats to emit 22-kHz ultrasonic vocalizations. However, no 22-kHz vocalizations were found in female rats tested. Visual function, serum corticosterone, and testosterone levels did not show significant differences between responders and non-responders.
Article
Medicine, Research & Experimental
Cheryl Tyszkiewicz, Ingrid D. Pardo, Hayley N. Ritenour, Chang-Ning Liu, Chris Somps
Summary: The study revealed minimal to mild reactive astrocytosis likely associated with Purkinje cell degeneration in the cerebellum of 24-week-old CBA/J mice, which may impact motor function.
LABORATORY ANIMAL RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Crystal T. Bluette, Ahmed M. Shoieb, Qinghai Peng, Balasubramanian Manickam, Wenhu Huang, Eyoung Shin, Wei Zhang, Young-Ho Song, Chang-Ning Liu
Summary: The study found that the use of nano-VCR may alleviate behavioral changes in CiPN, but does not improve structural changes in the peripheral nerve. Empty nanoparticles did not cause any behavioral, microRNA, or structural changes.
INTERNATIONAL JOURNAL OF TOXICOLOGY
(2021)
Article
Neurosciences
Yang He, Jun Tang, Meng Zhang, Junjie Ying, Dezhi Mu
Summary: This study investigated the protective effects and mechanisms of human placenta derived mesenchymal stem cells (hPMSCs) transplantation in a rat model of hypoxic-ischemic encephalopathy (HIE). The results showed that hPMSCs transplantation reduced apoptosis and improved long-term neurological prognosis. Furthermore, the downregulation of Sema 3A/NRP-1 expression and activation of the PI3K/Akt/mTOR signaling pathway played a key role in the protective effects of hPMSCs.
Article
Neurosciences
Emily L. Isenstein, Edward G. Freedman, Jiayi Xu, Ian A. DeAndrea-Lazarus, John J. Foxe
Summary: This study evaluated electrophysiological discrimination of parametric somatosensory stimuli in healthy young adults to understand how the brain processes the duration of tactile information. The results showed that participants did not electrophysiologically discriminate between 100 and 115 ms, but they exhibited distinct electrophysiological responses when the deviant stimuli were 130, 145, and 160 ms. These findings contribute to a better understanding of tactile sensitivity in different clinical conditions.
Article
Neurosciences
Juliana R. Souza, Ludmila Lima-Silveira, Daniela Accorsi-Mendonca, Benedito H. Machado
Summary: This study demonstrates that A2A receptors play a crucial role in modulating synaptic transmission in the NTS neurons and are required for the enhancement of glutamatergic transmission observed under short-term sustained hypoxia conditions.
Article
Neurosciences
Miki Hashizume, Rina Ito, Rie Suge, Yasushi Hojo, Gen Murakami, Takayuki Murakoshi
Summary: The basolateral amygdaloid complex (BLA) is closely involved in the formation of emotional memories, including both aversive memory and contextual fear memory. Acute sleep deprivation (SD) disrupts the acquisition of tone-associated fear memory in juvenile rats, but has no significant effect on contextual fear memory. Slow network oscillation in the amygdala contributes to the formation of amygdala-dependent fear memory in relation to sleep.
Article
Neurosciences
Qunxian Wang, Shipeng Guo, Dongjie Hu, Xiangjun Dong, Zijun Meng, Yanshuang Jiang, Zijuan Feng, Weihui Zhou, Weihong Song
Summary: GSDME plays a crucial role in the pathogenesis of Alzheimer's disease by regulating the switch from apoptosis to pyroptosis and participating in neuroinflammatory response. Knockdown of GSDME has been shown to improve cognitive impairments, indicating that GSDME could be a therapeutic target for Alzheimer's disease.