Article
Cell Biology
Daniel Hellenbrand, Clayton L. Haldeman, Jae-Sung Lee, Angela G. Gableman, Elena K. Dai, Stephen D. Ortmann, Jerrod C. Gotchy, Kierra K. Miller, Adrianna M. Doucas, Nicole C. Nowak, William L. Murphy, Amgad S. Hanna
Summary: The gold standard for treating peripheral nerve injuries with large nerve gaps is autologous nerve grafts. However, incorporating mineral coated microparticles (MCMs) to release growth factors like NGF and GDNF can significantly enhance axon growth and improve limb function in rats with nerve grafts.
NEURAL REGENERATION RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Donghee Lee, Navatha Shree Sharma, S. M. Shatil Shahriar, Kai Yang, Zheng Yan, Jingwei Xie
Summary: Electrospun nanofibers have found wide applications in neurobiology studies due to their biomimetic properties. However, current in vitro culture systems lack control of cell-nanofiber interactions and monitoring of single cell behavior. To address these challenges, researchers have developed a unique nanofiber embedded microfluidic device that combines microfluidic technology with electrospun nanofibers to study interactions between neurons and nanofiber materials. The device allows testing of how different topographies affect axonal growth and conducting laser-based axotomy to investigate axonal regeneration. This device can be a valuable tool for investigating nerve injury mechanisms and high-throughput screening of biomaterials or drugs for nerve repair, and can be applied in the design of medical devices such as nerve conduits for effective nerve regeneration.
Article
Chemistry, Multidisciplinary
Yizhu Shan, Lingling Xu, Xi Cui, Engui Wang, Fengying Jiang, Jiaxuan Li, Han Ouyang, Tailang Yin, Hongqing Feng, Dan Luo, Yan Zhang, Zhou Li
Summary: A responsive cascade drug delivery scaffold (RCDDS) has been developed to dynamically adjust drug release kinetics based on the patient's condition, promoting the treatment of peripheral nerve injury.
MATERIALS HORIZONS
(2023)
Article
Cell Biology
Ryan A. Hough, Timothee Pale, Jessica A. Benes, Andrew D. McClellan
Summary: Following spinal cord injury, injured lamprey RS neurons showed significant changes in firing patterns and biophysical properties, which are expected to promote axonal regeneration. The excitability and spiking frequencies of injured neurons were reduced compared to uninjured neurons.
Review
Neurosciences
Xiang Cui, Kun Liu, Xinyan Gao, Bing Zhu
Summary: This review discusses the functional plasticity of acupoints in acupuncture treatment and the role of activated C nociceptors.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Multidisciplinary Sciences
Miguel A. Rubio, Mireia Herrando-Grabulosa, Nuria Gaja-Capdevila, Jorge J. Vilches, Xavier Navarro
Summary: The loss of cutaneous small fibers, characteristic of ALS patients, has also been observed in SOD1(G93A) mice. This study aims to characterize the involvement of different somatosensory neuron populations in the mice and analyze the temporal progression of the sensory involvement. The findings suggest that cutaneous sensory axons are affected in SOD1(G93A) mice, with non-peptidergic axons being more vulnerable than peptidergic axons.
SCIENTIFIC REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Rabia Akram, Haseeb Anwar, Muhammad Shahid Javed, Azhar Rasul, Ali Imran, Shoaib Ahmad Malik, Chand Raza, Ikram Ullah Khan, Faiqa Sajid, Tehreem Iman, Tao Sun, Hyung Soo Han, Ghulam Hussain
Summary: Axons in the peripheral nervous system have the ability to repair themselves after damage, while axons in the central nervous system cannot. Intrinsic growth factors, proteomic profile, microtubule stability, and signaling pathways play significant roles in axonal regeneration. However, glial scar development, myelin-associated inhibitor molecules, lack of neurotrophic factors, and inflammatory reactions restrict axonal regeneration.
Article
Multidisciplinary Sciences
Paula M. Calvo, Rosendo G. Hernandez, Rosa R. de la Cruz, Angel M. Pastor
Summary: VEGF is a substance that has both angiogenic and neurotrophic activities. This study found that VEGF deprivation through retrograde transport affected the physiology of intact abducens motoneurons, causing changes in firing pattern, eye position, and eye velocity sensitivity, as well as a reduction in the density of afferent synaptic terminals. These findings demonstrate the essential role of retrogradely delivered VEGF in motoneuronal synaptic drive and discharge activity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Food Science & Technology
Chun-Yu Lin, Yi-Ju Chen, Chih-Hsuan Hsu, Yu-Hsuan Lin, Peng-Tzu Chen, Ting-Han Kuo, Chris T. Ho, Hsin-Hua Chen, Sih-Jia Huang, Ho-Chieh Chiu, Chin-Chu Chen, Eric Hwang
Summary: Erinacines derived from Hericium erinaceus have been found to possess neuro-protective effects against neurodegenerative diseases. This study discovered a previously unknown effect of erinacine S on enhancing neurite outgrowth and promoting axon regeneration of peripheral nervous system neurons as well as overcoming regeneration obstacles of central nervous system neurons. The research also revealed that erinacine S can cause the accumulation of neurosteroids in neurons.
JOURNAL OF FOOD AND DRUG ANALYSIS
(2023)
Article
Biology
Laura J. Wagstaff, Jose A. Gomez-Sanchez, Shaline Fazal, Georg W. Otto, Alastair M. Kilpatrick, Kirolos Michael, Liam Y. N. Wong, Ki H. Ma, Mark Turmaine, John Svaren, Tessa Gordon, Peter Arthur-Farraj, Sergio Velasco-Aviles, Hugo Cabedo, Cristina Benito, Rhona Mirsky, Kristjan R. Jessen
Summary: After nerve injury, myelin and Remak Schwann cells reprogram to repair cells specialized for regeneration but fail in aging animals and during chronic denervation. Restoring Schwann cell c-Jun levels can restore regeneration to control levels, with potential gene candidates mediating this effect. This common mechanism of reduced c-Jun in Schwann cells regulates nerve repair success and failure during aging and chronic denervation.
Article
Anesthesiology
George Goodwin, Sheridan McMurray, Edward B. Stevens, Franziska Denk, Stephen B. McMahon
Summary: Na(v)1.7 is a promising drug target for pain treatment, but there is a mismatch between the analgesia produced by Na(v)1.7 loss-of-function and the peripherally restricted Na(v)1.7 inhibitors. The function of Na(v)1.7 in axonal propagation in nociceptors is not clearly defined.
Article
Multidisciplinary Sciences
Lili Xie, Yuqin Yin, Larry Benowitz
Summary: CNTF gene therapy indirectly promotes optic nerve regeneration by increasing the expression of CCL5, and its beneficial effects are independent of CNTFRα but are affected by neutrophil and monocyte infiltration. Modulating the expression of CCR5 can also influence the effects of CNTF gene therapy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Materials Science, Biomaterials
Donghee Lee, Kai Yang, Jingwei Xie
Summary: Regeneration and functional recovery of damaged nerves are challenging. Microfluidic devices in combination with injury techniques have been applied to test biological hypotheses in nerve injury and regeneration. In this article, current in vitro nerve injury models on a chip that mimic in vivo axonal injuries and the regeneration process of axons are summarized.
Article
Immunology
Aaron D. Talsma, Jon P. Niemi, Joel S. Pachter, Richard E. Zigmond
Summary: "The study suggests that CCL2 is not essential for macrophage accumulation, myelin clearance, and axon regeneration in the peripheral nervous system. Other CCR2 chemokines, resident macrophage proliferation, and CCR2-independent monocyte recruitment can compensate for the absence of CCL2."
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Biochemistry & Molecular Biology
Lilian do Amaral, Neife Aparecida Guinaim dos Santos, Flavia Malvestio Sisti, Elaine Del Bel, Antonio Cardozo dos Santos
Summary: This study demonstrates the neurotrophic potential of doxycycline by inducing neurite outgrowth through activation of the trkA receptor and downstream signaling pathways, without inducing the expression of NGF. Additionally, doxycycline increased the expression of proteins involved in axonal and synaptic plasticity, suggesting a potential role in restoring neuronal connectivity lost at the initial phase of neurodegeneration.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Article
Neurosciences
Nicole M. Wiedmann, Agnes W. Wong, Janet R. Keast, Peregrine B. Osborne
Summary: The study used c-Fos and EGR-1 (Zif268) activity mapping to investigate cystometry-induced micturition in awake female and male rats, revealing sex differences. Females showed activation in dorsal horn laminae II-IV, while males exhibited net inhibition. The findings extend understanding of the functional molecular anatomy of the lower urinary tract-related circuit in the spinal cord and identify sex differences not detected in previous studies on anesthetized rats.
JOURNAL OF COMPARATIVE NEUROLOGY
(2021)
Article
Anatomy & Morphology
Martin M. Bertrand, Nadja Korajkic, Peregrine B. Osborne, Janet R. Keast
JOURNAL OF ANATOMY
(2020)
Article
Neurosciences
Sophie C. Payne, Nicole M. Wiedmann, Calvin D. Eiber, Agnes W. Wong, Philipp Senn, Peregrine B. Osborne, Janet R. Keast, James B. Fallon
FRONTIERS IN NEUROSCIENCE
(2020)
Article
Developmental Biology
Casey J. A. Smith-Anttila, Victoria Morrison, Janet R. Keast
Summary: The primary function of the urinary bladder is to store urine until a suitable time for voiding, which is determined by the coordinated activation of sensory and motor components of the nervous system. Innervation of the bladder begins at E13 during development, progressing dorsoventrally, with urothelial innervation occurring more slowly than muscle innervation.
DEVELOPMENTAL BIOLOGY
(2021)
Letter
Obstetrics & Gynecology
E. M. Colgrave, S. Bittinger, M. Healey, U. P. Dior, P. A. W. Rogers, J. R. Keast, J. E. Girling, S. J. Holdsworth-Carson
HUMAN REPRODUCTION
(2021)
Article
Obstetrics & Gynecology
Eliza M. Colgrave, Janet R. Keast, Sophie Bittinger, Martin Healey, Peter A. W. Rogers, Sarah J. Holdsworth-Carson, Jane E. Girling
Summary: Endometriosis is a heterogeneous disease in terms of patient symptoms, treatment responsiveness, and lesion presentation. Further exploration of the histological features of endometriotic lesions may improve treatment options. However, developing a comprehensive stratification system for such a diverse disease may be challenging.
HUMAN REPRODUCTION
(2021)
Article
Engineering, Biomedical
Calvin D. Eiber, Sophie C. Payne, Natalia P. Biscola, Leif A. Havton, Janet R. Keast, Peregrine B. Osborne, James B. Fallon
Summary: Neuromodulation of visceral nerves is being studied for various conditions, and computational tool ViNERS was developed to predict the impact of neuroanatomical variation on stimulation and recording from visceral nerves. The study demonstrated the potential of ViNERS to simulate large-scale stimulation and recording, while indicating the need for further refinement to accurately model visceral nerve axons.
JOURNAL OF NEURAL ENGINEERING
(2021)
Editorial Material
Urology & Nephrology
Claire C. Yang, James A. Hokanson, Janet R. Keast
NEUROUROLOGY AND URODYNAMICS
(2022)
Article
Multidisciplinary Sciences
Emanuele Plebani, Natalia P. Biscola, Leif A. Havton, Bartek Rajwa, Abida Sanjana Shemonti, Deborah Jaffey, Terry Powley, Janet R. Keast, Kun-Han Lu, M. Murat Dundar
Summary: This paper presents a high-throughput processing pipeline prototype for automated segmentation of unmyelinated fibers. The authors used transmission electron microscopy images of vagus and pelvic nerves in rats and trained a deep instance segmentation network using individually annotated unmyelinated axons. The algorithm achieved good segmentation results in both stand-alone mode and expert-in-the-loop hybrid setting.
SCIENTIFIC REPORTS
(2022)
Article
Neurosciences
J. P. Fuller-Jackson, P. B. Osborne, J. R. Keast
Summary: The sensorimotor circuits of the lumbosacral spinal cord play a crucial role in regulating lower urinary tract and pelvic pain states. Studies have shown that the innervation patterns of bladder and urethra afferents in the spinal cord are remarkably similar, with no significant structural sex differences observed in male and female rats. This research provides valuable insights for future studies on plasticity, injury responses, and modeling of these pathways.
Article
Neurosciences
Xinhong Chen, Sripriya Ravindra Kumar, Cameron D. Adams, Daping Yang, Tongtong Wang, Damien A. Wolfe, Cynthia M. Arokiaraj, Victoria Ngo, Lillian J. Campos, Jessica A. Griffiths, Takako Ichiki, Sarkis K. Mazmanian, Peregrine B. Osborne, Janet R. Keast, Cory T. Miller, Andrew S. Fox, Isaac M. Chiu, Viviana Gradinaru
Summary: Gene therapy shows promise in treating neuropathologies of the central and peripheral nervous systems. Researchers have developed and validated two gene delivery vectors, AAV-MaCPNS1 and AAV-MaCPNS2, in rodents and non-human primates. These vectors efficiently transduced the nervous systems of these species and demonstrated their functional utility through experiments in mice.
Article
Engineering, Biomedical
Jing Yang Liu, David B. Grayden, Janet R. Keast, Sam E. John
Summary: This study investigates the feasibility of stimulating peripheral nerves with an endovascular stent-mounted electrode array through computational models. The results suggest that the stimulation thresholds of the tested configurations are comparable to ring electrodes and depend on the inter-electrode distance and device orientation. Arranging multiple electrodes along the longitudinal axis of the nerve reduces surface charge density without sacrificing axon recruitment, while arranging electrodes along the circumference of the blood vessel reduces misalignment risk but lowers axon recruitment.
JOURNAL OF NEURAL ENGINEERING
(2023)
Article
Neurosciences
Abida Sanjana Shemonti, Emanuele Plebani, Natalia P. Biscola, Deborah M. Jaffey, Leif A. Havton, Janet R. Keast, Alex Pothen, M. Murat Dundar, Terry L. Powley, Bartek Rajwa
Summary: A thorough understanding of the neuroanatomy of peripheral nerves is required for a better insight into their function and the development of neuromodulation tools and strategies. In this study, we utilized spatial statistics and point process models to describe the axonal arrangement in peripheral nerves and used Sinkhorn distances to compute the similarities between different nerve cross-sections. Our findings demonstrate a novel approach to quantifying similarities between spatial point patterns and provide a generalizable pipeline for quantitative analysis of peripheral nerve architecture. Additionally, our data reveal differences between male- and female-originating samples and similarities between the pelvic and abdominal vagus nerves.
FRONTIERS IN NEUROSCIENCE
(2023)
Article
Engineering, Biomedical
Sophie C. Payne, Peregrine B. Osborne, Alex Thompson, Calvin D. Eiber, Janet R. Keast, James B. Fallon
Summary: This study presents a minimally invasive recording and analysis technology that can extract low-to-negative signal to noise ratio neural activity from fragile peripheral visceral nerves. The technology was tested and validated using a rat model, showing its potential in developing closed-loop technology for bladder control.
APL BIOENGINEERING
(2023)
Proceedings Paper
Engineering, Biomedical
JingYang Liu, David B. Grayden, Janet R. Keast, Sam E. John
Summary: Implantable neuromodulation devices interfacing with the peripheral nervous system show promise in restoring lost functions due to nerve damage. This study demonstrates the feasibility of delivering electrical stimulation of a peripheral nerve from a blood vessel and highlights the importance of considering nerve-vessel distance and relative orientation when screening candidate vessels for electrode implantation.
2021 43RD ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE & BIOLOGY SOCIETY (EMBC)
(2021)
Article
Neurosciences
Yang He, Jun Tang, Meng Zhang, Junjie Ying, Dezhi Mu
Summary: This study investigated the protective effects and mechanisms of human placenta derived mesenchymal stem cells (hPMSCs) transplantation in a rat model of hypoxic-ischemic encephalopathy (HIE). The results showed that hPMSCs transplantation reduced apoptosis and improved long-term neurological prognosis. Furthermore, the downregulation of Sema 3A/NRP-1 expression and activation of the PI3K/Akt/mTOR signaling pathway played a key role in the protective effects of hPMSCs.
Article
Neurosciences
Emily L. Isenstein, Edward G. Freedman, Jiayi Xu, Ian A. DeAndrea-Lazarus, John J. Foxe
Summary: This study evaluated electrophysiological discrimination of parametric somatosensory stimuli in healthy young adults to understand how the brain processes the duration of tactile information. The results showed that participants did not electrophysiologically discriminate between 100 and 115 ms, but they exhibited distinct electrophysiological responses when the deviant stimuli were 130, 145, and 160 ms. These findings contribute to a better understanding of tactile sensitivity in different clinical conditions.
Article
Neurosciences
Juliana R. Souza, Ludmila Lima-Silveira, Daniela Accorsi-Mendonca, Benedito H. Machado
Summary: This study demonstrates that A2A receptors play a crucial role in modulating synaptic transmission in the NTS neurons and are required for the enhancement of glutamatergic transmission observed under short-term sustained hypoxia conditions.
Article
Neurosciences
Miki Hashizume, Rina Ito, Rie Suge, Yasushi Hojo, Gen Murakami, Takayuki Murakoshi
Summary: The basolateral amygdaloid complex (BLA) is closely involved in the formation of emotional memories, including both aversive memory and contextual fear memory. Acute sleep deprivation (SD) disrupts the acquisition of tone-associated fear memory in juvenile rats, but has no significant effect on contextual fear memory. Slow network oscillation in the amygdala contributes to the formation of amygdala-dependent fear memory in relation to sleep.
Article
Neurosciences
Qunxian Wang, Shipeng Guo, Dongjie Hu, Xiangjun Dong, Zijun Meng, Yanshuang Jiang, Zijuan Feng, Weihui Zhou, Weihong Song
Summary: GSDME plays a crucial role in the pathogenesis of Alzheimer's disease by regulating the switch from apoptosis to pyroptosis and participating in neuroinflammatory response. Knockdown of GSDME has been shown to improve cognitive impairments, indicating that GSDME could be a therapeutic target for Alzheimer's disease.
