Article
Cell Biology
Juliana E. Gentile, Melissa G. Carrizales, Anthony J. Koleske
Summary: This article reviews the historical understanding of synaptic actin and highlights its key role in neuronal function. It discusses the organization and regulation of synaptic actin, as well as genetic variants related to neurodevelopmental and psychiatric disorders.
Review
Neurosciences
Kate L. Prichard, Nicholas S. O'Brien, Sari R. Murcia, Jennifer R. Baker, Adam McCluskey
Summary: Endocytosis is a crucial process for cell health and signaling, specifically clathrin mediated endocytosis (CME) plays a key role in synaptic vesicle recycling and intracellular communication in the brain. This review focuses on clathrin and dynamin, two proteins involved in CME, and discusses the potential therapeutic interventions and inhibitors for conditions involving defects in CME.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Daniele Lana, Elisabetta Gerace, Giada Magni, Francesca Cialdai, Monica Monici, Guido Mannaioni, Maria Grazia Giovannini
Summary: The study revealed that microglia undergo rapid changes in phenotype in response to oxygen-glucose deprivation in rat organotypic hippocampal slices, depending on factors such as location and time, and play different roles in damaged neurons.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Sarah M. Smith, Gabrielle Larocque, Katherine M. Wood, Kyle L. Morris, Alan M. Roseman, Richard B. Sessions, Stephen J. Royle, Corinne J. Smith
Summary: The interaction between Clathrin and the AP2 complex plays a crucial role in coated-pit assembly, with a beta 2 appendage able to bind in at least two positions in the clathrin cage. This multi-modal binding is shown to be a fundamental property of clathrin-AP2 interactions.
Article
Oncology
Lisa R. Decotret, Rocky Shi, Kiersten N. Thomas, Manchi Hsu, Catherine J. Pallen, Kevin L. Bennewith
Summary: We present an optimized ex vivo brain slice invasion assay for modeling glioblastoma multiforme (GBM) cell invasion into brain tissue. Our novel imaging technique allows for the visualization of invasive structures beneath GBM spheroids using confocal microscopy and an ImageJ macro for quantification. Compared to in vitro Matrigel invasion, GBM cells show distinct modes of motility when invading brain tissue, highlighting the importance of studying GBM invasion in the brain microenvironment.
FRONTIERS IN ONCOLOGY
(2023)
Article
Neurosciences
Agata Idziak, V. V. G. Krishna Inavalli, Stephane Bancelin, Misa Arizono, U. Valentin Nagerl
Summary: Chemical fixation is a standard step for preserving cells and tissues for microscopic analyses, but it may cause physical alterations in cellular proteins and membranes. Using super-resolution microscopy, this study investigated the acute effects of paraformaldehyde (PFA) on tissue integrity and found subtle changes in dendritic spine morphology and substantial damage to cell membranes.
Article
Biochemistry & Molecular Biology
Alexander Johnson, Walter A. Kaufmann, Christoph Sommer, Tommaso Costanzo, Dana A. Dahhan, Sebastian Y. Bednarek, Jiri Friml
Summary: Biological systems are made up of dynamic three-dimensional parts. This study introduces novel methods to analyze biological structures and focuses on Arabidopsis clathrin-coated vesicles (CCVs). The methods allow for visualization of CCVs in unroofed cells and biochemical isolated organelles at high resolution, and also provide a deep learning analysis method for screening CCVs in metal replicated samples.
Article
Cell Biology
Bo Shi, Ying-Hui Jin, Ling-Gang Wu
Summary: This study investigates the role of dynamin 1 in endocytosis and vesicle size regulation. The results show that knockout of dynamin 1 slows down endocytosis and increases the diameter of synaptic vesicles, suggesting an important function of dynamin 1 in controlling vesicle size during pit formation.
Article
Neurosciences
Pablo Vergara, Gabriela Pino, Jorge Vera, Felipe Arancibia, Magdalena Sanhueza
Summary: Prolonged changes in neural activity trigger homeostatic synaptic plasticity, allowing neuronal networks to operate within functional ranges. Deafferentation causes rapid and diverse compensations, which may contribute to understanding homeostatic or pathological events after brain injury.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Kyung Hee Lee, Un Jeng Kim, Myeounghoon Cha, Bae Hwan Lee
Summary: Increased oxidative damage in the brain with age causes abnormal brain function and disease. Ascorbic acid is an endogenous antioxidant that protects neurons, but its levels decrease with aging. Studies show that chronic ascorbate supplementation can reduce neuronal death and preserve the antioxidant system in the brain, protecting hippocampal neurons from age-related oxidative stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Janne Capra, Kai Harkonen, Heikki Kyykallio, Helena Vihinen, Eija Jokitalo, Kirsi Rilla
Summary: We used high-resolution imaging techniques to characterize the size and morphology of extracellular vesicles produced by breast cancer cells. The results showed diverse morphology of these vesicles and emphasized the importance of comparing different methods.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Darja Bozic, Matej Hocevar, Matic Kisovec, Manca Pajnic, Ljubisa Paden, Marko Jeran, Apolonija Bedina Zavec, Marjetka Podobnik, Ksenija Kogej, Ales Iglic, Veronika Kralj-Iglic
Summary: EVs have potential in diagnostics and therapy, but poor reproducibility in studies hinders practical application. Study on hbEVs stability reveals consistent morphology under specific conditions, making them suitable as EV reference material.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Maik Kahnt, Lukas Grote, Dennis Brueckner, Martin Seyrich, Felix Wittwer, Dorota Koziej, Christian G. Schroer
Summary: Ptychographic X-ray microscopy is ideal for observing chemical processes in situ, but the thickness of samples can limit lateral resolution in traditional reconstruction models. By adopting a multi-slice reconstruction approach, higher spatial resolution can be achieved.
SCIENTIFIC REPORTS
(2021)
Article
Neurosciences
Alp Paksoy, Simone Hoppe, Yvette Doerflinger, Heinz Horstmann, Kurt Satzler, Christoph Koerber
Summary: This study investigated the role of Clathrin in synaptic vesicle recycling by using a Clathrin inhibitor in the auditory brainstem. The results demonstrate that Clathrin is involved in the recycling of synaptic vesicles from both the plasma membrane and large endosomes.
FRONTIERS IN SYNAPTIC NEUROSCIENCE
(2022)
Article
Chemistry, Analytical
Amirus Saleheen, Debalina Acharyya, Rebecca A. Prosser, Christopher A. Baker
Summary: Ex vivo brain slice cultures are commonly used in neurophysiology research, with microfluidic perfusion systems showing promise for maintaining tissue viability. A novel microfluidic bubble perfusion device was developed to address the challenges of high oxygen demand in brain slices, successfully maintaining murine hypothalamic tissue slices for up to 10 hours.
ANALYTICAL METHODS
(2021)
Article
Cell Biology
Khuloud Jaqaman, James A. Galbraith, Michael W. Davidson, Catherine G. Galbraith
MOLECULAR BIOLOGY OF THE CELL
(2016)
Editorial Material
Cell Biology
Catherine G. Galbraith, James A. Galbraith
JOURNAL OF CELL SCIENCE
(2011)
Article
Biochemical Research Methods
Hari Shroff, Catherine G. Galbraith, James A. Galbraith, Eric Betzig
Article
Biochemical Research Methods
Thomas A. Planchon, Liang Gao, Daniel E. Milkie, Michael W. Davidson, James A. Galbraith, Catherine G. Galbraith, Eric Betzig
Article
Multidisciplinary Sciences
Xiaobing Chen, Christine Winters, Rita Azzam, Xiang Li, James A. Galbraith, Richard D. Leapman, Thomas S. Reese
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2008)
Article
Multidisciplinary Sciences
Gleb Shtengel, James A. Galbraith, Catherine G. Galbraith, Jennifer Lippincott-Schwartz, Jennifer M. Gillette, Suliana Manley, Rachid Sougrat, Clare M. Waterman, Pakorn Kanchanawong, Michael W. Davidson, Richard D. Fetter, Harald F. Hess
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2009)
Review
Nanoscience & Nanotechnology
James A. Galbraith, Catherine G. Galbraith
WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
(2011)
Article
Biology
Catherine G. Galbraith, Michael W. Davidson, James A. Galbraith
Article
Multidisciplinary Sciences
Hari Shroff, Catherine G. Galbraith, James A. Galbraith, Helen White, Jennifer Gillette, Scott Olenych, Michael W. Davidson, Eric Betzig
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2007)
Article
Multidisciplinary Sciences
Catherine G. Galbraith, Kenneth M. Yamada, James A. Galbraith
Article
Cell Biology
Joseph A. DeGiorgis, James A. Galbraith, Ayse Dosemeci, Xiaobing Chen, Thomas S. Reese
BRAIN CELL BIOLOGY
(2006)
Article
Cell Biology
JA Galbraith, M Terasaki
MOLECULAR BIOLOGY OF THE CELL
(2003)
Review
Cell Biology
JA Galbraith, PE Gallant
JOURNAL OF NEUROCYTOLOGY
(2000)
Article
Neurosciences
Yang He, Jun Tang, Meng Zhang, Junjie Ying, Dezhi Mu
Summary: This study investigated the protective effects and mechanisms of human placenta derived mesenchymal stem cells (hPMSCs) transplantation in a rat model of hypoxic-ischemic encephalopathy (HIE). The results showed that hPMSCs transplantation reduced apoptosis and improved long-term neurological prognosis. Furthermore, the downregulation of Sema 3A/NRP-1 expression and activation of the PI3K/Akt/mTOR signaling pathway played a key role in the protective effects of hPMSCs.
Article
Neurosciences
Emily L. Isenstein, Edward G. Freedman, Jiayi Xu, Ian A. DeAndrea-Lazarus, John J. Foxe
Summary: This study evaluated electrophysiological discrimination of parametric somatosensory stimuli in healthy young adults to understand how the brain processes the duration of tactile information. The results showed that participants did not electrophysiologically discriminate between 100 and 115 ms, but they exhibited distinct electrophysiological responses when the deviant stimuli were 130, 145, and 160 ms. These findings contribute to a better understanding of tactile sensitivity in different clinical conditions.
Article
Neurosciences
Juliana R. Souza, Ludmila Lima-Silveira, Daniela Accorsi-Mendonca, Benedito H. Machado
Summary: This study demonstrates that A2A receptors play a crucial role in modulating synaptic transmission in the NTS neurons and are required for the enhancement of glutamatergic transmission observed under short-term sustained hypoxia conditions.
Article
Neurosciences
Miki Hashizume, Rina Ito, Rie Suge, Yasushi Hojo, Gen Murakami, Takayuki Murakoshi
Summary: The basolateral amygdaloid complex (BLA) is closely involved in the formation of emotional memories, including both aversive memory and contextual fear memory. Acute sleep deprivation (SD) disrupts the acquisition of tone-associated fear memory in juvenile rats, but has no significant effect on contextual fear memory. Slow network oscillation in the amygdala contributes to the formation of amygdala-dependent fear memory in relation to sleep.
Article
Neurosciences
Qunxian Wang, Shipeng Guo, Dongjie Hu, Xiangjun Dong, Zijun Meng, Yanshuang Jiang, Zijuan Feng, Weihui Zhou, Weihong Song
Summary: GSDME plays a crucial role in the pathogenesis of Alzheimer's disease by regulating the switch from apoptosis to pyroptosis and participating in neuroinflammatory response. Knockdown of GSDME has been shown to improve cognitive impairments, indicating that GSDME could be a therapeutic target for Alzheimer's disease.