Journal
NEUROREPORT
Volume 20, Issue 4, Pages 354-359Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/WNR.0b013e328323d74c
Keywords
coculture; differentiation; Schwann cell; umbilical cord blood
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Funding
- Natural Science Found of China [U0632008, 30270491, 30772232, 30500526]
- Guangdong Province [(2004) 08, (2007) 05/06-7005206, (2003)209, (2008)258-2008A030201019]
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In this study, we examined the phenotypic and bioassay characteristics of human umbilical cord blood-derived mesenchymal stromal cells (UCB-MSCs) differentiated along a Schwann cells lineage. Initially, we induced human UCB-MSCs into floating neurospheres, and then, neurospheres were induced to differentiate into Schwann-like cells using glia growth factors. Differentiated UCB-MSCs showed morphological changes similar to those of Schwann cells. Expression of the Schwann cell markers was determined by immunocytochemical staining and western blotting. Furthermore, differentiated UCB-MSCs could promote neurite outgrowth in coculture with dorsal root ganglia neurons. These results show that UCB-MSCs can be differentiated into cells that are Schwann-like in terms of morphology, phenotype, and function. NeuroReport 20:354-359 (C) 2009 Wolters Kluwer Health | Lippincott Williams & Wilkins.
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