Article
Agriculture, Multidisciplinary
Zhong Qiang Jia, Su Gui Zhang, Ying Wang, Jun Heng Pan, Fei Fan Liu, En Ling Zhan, Eman Atef Fouad, Ya Li Fu, Qi Rui Pan, Chun Qing Zhao
Summary: SlRdl1 and SlRdl2 are essential for GABA-induced neural signal transmission in Spodoptera litura. Knockout of SlRdl1 and SlRdl2 resulted in increased mortality and prolonged larval and pupal duration. Only knockout of SlRdl1 affected flight capability, significantly increasing flight time and distance. The study provides new insights into lepidopteran insect ionotropic γ-aminobutyric acid receptors (iGABARs) research.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Neurosciences
Steffen Fricke, Magnus Harnau, Florian Hetsch, Haoran Liu, Julia Leonhard, Anna Eylmann, Pina Knauff, Han Sun, Marcus Semtner, Jochen C. Meier
Summary: High concentrations of cesium can have adverse effects on neuronal physiology, prompting the FDA to issue a warning. It has been found that cesium activates glycine receptors, and further investigation revealed the binding profiles of cesium and potassium to these receptors through experiments and simulations.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2023)
Article
Neurosciences
Braulio Munoz, Trinidad Mariqueo, Pablo Murath, Christian Peters, Gonzalo E. Yevenes, Gustavo Moraga-Cid, Robert W. Peoples, Luis G. Aguayo
Summary: The study identified a new modulatory role of beta subunits on ethanol sensitivity of alpha 2 GlyRs, as well as differential allosteric modulation in response to ethanol, GTP-γ-S, propofol, zinc ions, and trichloroethanol in homomeric and heteromeric conformations of alpha 2 GlyRs.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2021)
Article
Pharmacology & Pharmacy
Nawid Madjroh, Paul A. Davies, Joshua L. Smalley, Uffe Kristiansen, Pella C. Soderhielm, Anders A. Jensen
Summary: The Zinc-Activated Channel (ZAC), a member of the Cys-loop receptor (CLR) superfamily, exhibits distinct functional properties due to the Thr(128) Ala variation. While ZAC(Thr128) and ZAC(Ala128) show similar expression characteristics in transfected mammalian cells, their different functionalities may arise from the latter. The 1-hydroxyethyl sidechain of Thr(128) plays a subtle role in the functional importance of ZAC.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Developmental Biology
Andreas Heim, Marie L. Niedermeier, Florian Stengel, Thomas U. Mayer
Summary: This study elucidates the role of Zar1l in Xenopus oocytes, showing that it accelerates hormone-induced meiotic resumption. It is found that Zar1l forms a ribonucleoparticle complex with other proteins to regulate the translation of specific mRNAs, controlling key cell cycle regulators during oocyte maturation.
Review
Oncology
Ji-Long Liu, Joseph G. Gall
Summary: Novel spherical structures called C-bodies are induced by cold shock on lampbrush chromosomes of Xenopus laevis oocytes, showing symmetrical distribution on homologous LBCs similar to 5S rDNA pattern. Neither active transcription nor translation is required for their formation. The function of C-bodies remains unknown.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Alexander A. Tokmakov, Ken-Ichi Sato
Summary: This study found that intracellular ROS levels increase in aged frog oocytes and eggs, and that targeted inhibition of NADPH oxidase with apocynin significantly reduces ROS levels, maintaining normal cell function.
Article
Multidisciplinary Sciences
Bastien Le Guellec, France Rousseau, Marion Bied, Stephane Supplisson
Summary: ATB(0,+) is a member of the SLC6 family that functions as an amino acid transporter with broad substrate specificity. It exhibits a 3:1:1 Na+ :Cl- :Gly stoichiometry and behaves as a unidirectional transporter, with virtually no efflux of glycine at negative potentials. Its charge movement analysis reveals a higher affinity for sodium compared to GlyT2, and a gating mechanism that locks sodium into the transporter at depolarized potentials.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Richard Agren, Tomasz Maciej Stepniewski, Hugo Zeberg, Jana Selent, Kristoffer Sahlholm
Summary: The study used an ion channel activation assay to estimate the rate constants of dopamine D-2 receptor agonists, with results showing higher k(on) for (S)-5-OH-DPAT compared to (R)-5-OH-DPAT, and similar k(off) for both enantiomers at wild-type D2R. Through mutagenesis, the k(on) of (S)-5-OH-DPAT can be reduced, decreasing the potency difference between the two enantiomers. The derived kinetic K(d)s correlated well with EC50 values for all compounds, indicating the assay captured meaningful information about binding kinetics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
Sicong Yu, Lepeng Gao, Yang Song, Xin Ma, Shuang Liang, Hainan Lan, Xin Zheng, Suo Li
Summary: In this study, a mitochondrial damage model was induced in porcine oocytes with the Bcl-2-specific antagonist ABT-199, and the protective effects of glycine (Gly) against ABT-199-induced mitochondrial dysfunction were investigated. Gly was found to significantly ameliorate mitochondrial dysfunction, oxidative stress, and apoptosis, as well as regulate intracellular calcium levels and IP(3)R1 cellular distribution, thereby protecting oocyte maturation.
JOURNAL OF ANIMAL SCIENCE
(2021)
Article
Environmental Sciences
Lepeng Gao, Chang Zhang, Sicong Yu, Shuang Liu, Guoxia Wang, Hainan Lan, Xin Zheng, Suo Li
Summary: This study investigated the effects of mono-n-butyl phthalate (MBP) on the in vitro maturation (IVM) and development of porcine oocytes using SMART-seq technology. The results showed that differentially expressed genes were enriched in cytoskeleton, cell apoptosis, endoplasmic reticulum (ER), and mitochondria. Glycine (Gly) was found to improve oocyte developmental potential by regulating mitochondrial and ER function. Gly supplementation significantly reduced MBP-induced spindle abnormalities and mitochondria-associated ER membrane (MAM) interactions. Moreover, Gly treatment restored the distribution of certain proteins and genes, leading to decreased intracellular free calcium concentration, increased ER levels and mitochondrial membrane potential, reduced levels of reactive oxygen species, and increased levels of Glutathione.
ENVIRONMENTAL POLLUTION
(2022)
Article
Neurosciences
Kathryn R. Przybysz, Meredith E. Gamble, Marvin R. Diaz
Summary: The study reveals that adolescent alcohol exposure may result in long-term sex-dependent effects on BLA physiology and KOR function in adulthood, impacting the modulation of GABAergic and glutamatergic neurotransmission.
Article
Biochemistry & Molecular Biology
Nikhil D. Aggarwal, Steven L. Zeng, Ryan J. Lashgari, Leland C. Sudlow, Mikhail Y. Berezin
Summary: A 3D Cultrex-based storage media was found to prolong the storage lifespan of Xenopus laevis oocytes and maintain their integrity and protein expression.
Article
Physiology
Kazutaka Ushio, Erika Watanabe, Takehiro Kamiya, Ayumi Nagashima, Tadaomi Furuta, Genki Imaizumi, Toru Fujiwara, Michael F. Romero, Akira Kato
Summary: Boric acid is a toxic micronutrient at high concentrations in animals. This study identifies aquaporins as potential boric acid channels in human cells, providing insights into the transport mechanisms of boric acid.
PHYSIOLOGICAL REPORTS
(2022)
Article
Pharmacology & Pharmacy
Anibal Araya, Scarlet Gallegos, Rodrigo Viveros, Loreto San Martin, Braulio Munoz, Robert J. Harvey, Hanns U. Zeilhofer, Luis G. Aguayo
Summary: The study reveals that both VTA and PFC contain similar levels of α and β receptor subunits, with lower sensitivity to glycine in PFC compared to VTA. Ethanol potentiates glycine receptors in VTA neurons but not in PFC neurons. Both regions express heteromeric αβ receptors, with α1 glycine receptors predominantly expressed in VTA and α2/α3/α4 glycine receptor subunits suggested in PFC.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Correction
Neurosciences
Lucia Privitera, Ellen L. Hogg, Matthias Gaestel, Mark J. Wall, Sonia A. L. Correa
Article
Neurosciences
Li-Ya Jiang, Guan-Hao Wang, Jing-Jiao Xu, Xiao-Li Li, Xiao-Yan Lin, Xiang Fang, Hong-Xu Zhang, Mei Feng, Chun-Ming Jiang
Summary: This study reveals the importance of LINC00473 in regulating temozolomide (TMZ) resistance in glioblastoma (GB) and its potential mechanism. By regulating the expression of CEBP alpha and MGMT, LINC00473 promotes the formation of chemoresistance. Furthermore, LINC00473 can transfer chemoresistance to adjacent sensitive cells through exosomes.
Article
Neurosciences
Olga Kopach, Tetyana Pivneva, Nataliya Fedirko, Nana Voitenko
Summary: This study found that diabetic animals exhibit severe xerostomia characterized by reduced saliva flow rate, diminished total protein content, and decreased amylase activity. The impaired saliva production in diabetes is associated with reduced and delayed intracellular Ca2+ signals in submandibular acinar cells, caused by malfunctioning mitochondria. Targeting malfunctioning mitochondria may be a potential strategy for the treatment of diabetic xerostomia.
Article
Neurosciences
Nicholas M. Timme, Cherish E. Ardinger, Seth D. C. Weir, Rachel Zelaya-Escobar, Rachel Kruger, Christopher C. Lapish
Summary: This study aimed to assess aversion-resistant drinking behavior in head-fixed mice and explore the relationship between non-consummatory behaviors and aversion-resistant drinking. The results showed that head-fixed mice exhibited heterogenous levels of aversion-resistant drinking and non-consummatory behaviors were related to the intensity of this behavior.
Article
Neurosciences
David R. Maguire, Charles P. France
Summary: Methocinnamox (MCAM) is a novel, long-acting opioid receptor antagonist that effectively decreases fentanyl self-administration and prevents opioid overdose in monkeys. The study demonstrates the potential therapeutic utility of MCAM in the treatment of opioid use disorder.
Article
Neurosciences
Xiang Li, Dan Feng, Shenglu Ma, Mingxing Li, Shulei Zhao, Man Tang
Summary: This study investigated the effects of fluoxetine on neurochemical, neurobiological, and neurobehavioral changes in different subregions of the hippocampus. The results showed that fluoxetine increased dialysate 5-HT, decreased membrane 5-HTT protein, and increased cytoplasmic fraction. Additionally, fluoxetine reduced immobility times in behavioral tests, with greater effects observed in the ventral subregion compared to the dorsal subregion.
Article
Neurosciences
Alexander V. Zholos, Mariia I. Melnyk, Dariia O. Dryn
Summary: Acetylcholine is an important neurotransmitter in visceral smooth muscles, activating M2 and M3 muscarinic receptors to cause smooth muscle excitation and contraction. This review focuses on the cellular and molecular mechanisms underlying acetylcholine-induced depolarisation and smooth muscle contraction, as well as the effects of anticholinergic drugs on gastrointestinal motility. The knowledge gained from recent studies has greatly expanded our understanding of these processes.
Article
Neurosciences
Zhenlong Li, Hsien-Yu Peng, Chau-Shoun Lee, Tzer-Bin Lin, Ming-Chun Hsieh, Cheng-Yuan Lai, Han-Fang Wu, Lih-Chyang Chen, Mei-Ci Chen, Dylan Chou
Summary: Methylone shows significant efficacy in treating depression and social deficits, making it an ideal candidate for anti-depressant medication.
Article
Neurosciences
Aline Freyssin, Allison Carles, Sarra Guehairia, Gilles Rubinstenn, Tangui Maurice
Summary: This study explores the potential of combining FENM and S1R agonists in the treatment of Alzheimer's disease. The results showed that most FENM-based combinations can protect against learning deficits caused by A beta 25-35, with better efficacy in short-term memory.
Article
Neurosciences
J. D. Lorente, J. Cuitavi, L. Rullo, S. Candeletti, P. Romualdi, L. Hipolito
Summary: This study analyzed the effects of pain on negative affect in different sexes and time courses, as well as the involvement of the dynorphinergic and corticotropin releasing factor systems in these pain-related behaviors. The results showed sex and time-dependent anxiety- and anhedonia-like behaviors induced by pain in female rats. The recruitment of KOR/DYN in the NAc was identified as a key neurological substrate mediating pain-induced behavioral alterations.
Article
Neurosciences
Rongjun Liu, Daofan Sun, Xiuzhong Xing, Qingge Chen, Bo Lu, Bo Meng, Hui Yuan, Lan Mo, Liufang Sheng, Jinwei Zheng, Qiusheng Wang, Junping Chen, Xiaowei Chen
Summary: The coexistence of pain and depression is frequently observed in patients with chronic pain and depression. Oxytocin, a neuropeptide, has been reported to relieve chronic pain and depressive symptoms. This study investigated the effect of intranasal oxytocin on neuropathic pain and comorbid depressive symptoms, and found that oxytocin attenuated depression-like behavior but did not alleviate mechanical hyperalgesia. The results suggest that intranasal oxytocin may have the potential to treat depressive symptoms in neuropathic pain patients.
