Article
Multidisciplinary Sciences
Bing Feng, Hesong Liu, Ila Mishra, Clemens Duerrschmid, Peiyu Gao, Pingwen Xu, Chunmei Wang, Yanlin He
Summary: Asprosin, a recently discovered adipokine, activates AgRP neurons in the ARH through binding to Ptprd, and the SK channel plays a crucial role in mediating this effect. Deficiency or elevation of circulating asprosin affects the SK current in AgRPARH neurons, and deletion of SK3 blocks asprosin-induced activation of AgRPARH neurons and overeating.
Article
Multidisciplinary Sciences
Willy Carrasquel-Ursulaez, Ignacio Segura, Ignacio Diaz-Franulic, Felipe Echeverria, Yenisleidy Lorenzo-Ceballos, Nicolas Espinoza, Maximiliano Rojas, Jose Antonio Garate, Eduardo Perozo, Osvaldo Alvarez, Fernando D. Gonzalez-Nilo, Ramon Latorre
Summary: This study demonstrates that two arginines in the transmembrane segment S4 function as the gating charges of BK channels, and the energy landscape of gating particles is tuned by a network of salt bridges in the voltage sensor domain (VSD). Unlike Kv channels, the charge movement in BK seems limited to a small displacement of the guanidinium moieties of R210 and R213.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Clinical Neurology
Donald Iain MacDonald, Ana P. Luiz, Federico Iseppon, Queensta Millet, Edward C. Emery, John N. Wood
Summary: Neuropathic pain leads to innocuous cooling being perceived as excruciating pain, yet the cell and molecular basis of this cold allodynia remains poorly understood. Research has found that in neuropathic pain states, normally silent large diameter cold-sensing neurons become sensitive to cooling, with many of them responding to noxious mechanical stimuli.
Review
Pharmacology & Pharmacy
Chiara Capitani, Ginevra Chioccioli Altadonna, Michele Santillo, Elena Lastraioli
Summary: Despite improvements in treatment, lung cancer continues to be a major global health problem. Adenocarcinoma is the most common subtype, while Small Cell Lung Cancer is the most challenging and difficult to treat. The standard care for these patients involves surgery, radiation therapy, and chemotherapy. The search for novel biomarkers that can aid in diagnosis and therapy is crucial.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Environmental Sciences
Zhenjie Sun, Wenhui Gu, Zezhong Feng, Yaqin Fan, Jianfeng Niu, Guangce Wang
Summary: This study elucidated the expression regulation mechanism of the heat shock protein 70 gene in Neopyropia yezoensis under high temperature stress. The findings showed the involvement of cAMP-dependent protein kinase and activating transcription factors element in the transcription regulation of the gene. In addition, AMPK was also found to play a role in the induction of the gene. Furthermore, posttranscriptional mechanisms were proposed to be involved in the synthesis regulation of the heat shock protein 70-2 protein.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Sahar Aminzai, Tingfei Hu, Renate B. Pilz, Darren E. Casteel
Summary: This study found that purified PKGI alpha can be oxidized and activated during storage, indicating that oxidation and trace metals present during storage play a role in PKGI alpha activation. Previous research suggested that certain amino acids in PKGI alpha are key for oxidation-induced activation. However, there is currently no direct evidence of oxidation-induced PKGI alpha activation in vivo.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Karolina Nowicka-Bauer, Monika Szymczak-Cendlak
Summary: Sperm motility is closely linked to the sensitivity and specificity of ion channels, with different ion channels interacting to ensure timely and efficient fertilization. The activation and modulation of these channels play key roles in regulating sperm motility in animals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Victor N. Ierusalimsky, Pavel M. Balaban, Evgeny S. Nikitin
Summary: Electrophysiological and genetic studies have identified two major subclasses of L5 neocortical pyramidal neurons, with one subclass expressing KCa3.1 channels and the other exhibiting weak afterhyperpolarization. This study demonstrates that Nav1.6 channels, rather than KCa3.1 channels, have an impact on the threshold, dynamics, and coding abilities of these neurons. The findings suggest a link between Nav1.6 function and the ability of L5 neurons to encode high frequencies.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Sara R. Roig, Silvia Cassinelli, Maria Navarro-Perez, Mireia Perez-Verdaguer, Irene Estadella, Jesusa Capera, Antonio Felipe
Summary: The Kv beta family is a group of modulators that regulate Kv channels, with Kv beta 2.1 clustering into immunological synapses during leukocyte activation. The localization of Kv beta 2.1 is mainly affected by S-acylation and a pair of C-terminal cysteines, and its targeting to lipid raft microdomains is influenced by various stimuli.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Hitesh Soni, Ravi Kumar, Praghalathan Kanthakumar, Adebowale Adebiyi
Summary: IL-1 beta induces Ca2+ entry via TRPA1 channels, leading to MAPK-dependent mesangial cell proliferation without stimulating fibronectin production.
Article
Neurosciences
Elva Martin-Batista, Laura E. Maglio, Natalia Armas-Capote, Guadalberto Hernandez, Diego Alvarez de la Rosa, Teresa Giraldez
Summary: SGK1.1 activation was found to significantly reduce levels of neuronal death and reactive glial activation induced by status epilepticus, synergistically with the regulation of cellular excitability, resulting in a significant reduction of seizure-induced brain damage in relevant areas.
NEUROBIOLOGY OF DISEASE
(2021)
Article
Cell Biology
Armando Jesus Perez-Diaz, Beatriz Vazquez-Marin, Jero Vicente-Soler, Francisco Prieto-Ruiz, Teresa Soto, Alejandro Franco, Jose Cansado, Marisa Madrid
Summary: The autophagy pathway in yeast is regulated differently in response to glucose limitation, indicating evolutionary variations in the signaling pathways that govern autophagy during glucose shortage or starvation.
Article
Multidisciplinary Sciences
D. Kent Arrell, Sungjo Park, Satsuki Yamada, Alexey E. Alekseev, Armin Garmany, Ryounghoon Jeon, Ivan Vuckovic, Jelena Zlatkovic Lindor, Andre Terzic
Summary: This study used multiomics surveillance and systems interrogation to study the cellular impact of Kir6.2 deficiency in the heart. The results showed that despite normal organ performance, the knockout hearts exhibited altered proteome and metabolome profiles, indicating that Kir6.2 deficiency can lead to metabolic reorganization and disease susceptibility.
SCIENTIFIC REPORTS
(2022)
Article
Neurosciences
Marina Arribas-Blazquez, Dolores Piniella, Luis A. Olivos-Ore, David Bartolome-Martin, Cristiana Leite, Cecilio Gimenez, Antonio R. Artalejo, Francisco Zafra
Summary: The voltage-sensitive sodium channel Na(V)1.1 is regulated by multiple protein kinases, with AKT1 identified as a novel regulator through direct phosphorylation. AKT1 activation leads to decreased Na+ currents and altered inactivation properties, mimicked by its specific activator SC79 and reverted by a selective inhibitor triciribine. This novel mechanism proposes AKT1 as a key regulator in modulating neuronal excitability under physiological and pathological conditions, including epileptogenesis.
Article
Biochemistry & Molecular Biology
Vitalyi Senyuk, Najmeh Eskandari, Ying Jiang, Rebeca Garcia-Varela, Rachel Sundstrom, Luigi Leanza, Roberta Peruzzo, Mark Burkard, Richard D. Minshall, Saverio Gentile
Summary: Stimulation of the Kv11.1 potassium channel activity in breast cancer cells leads to mitochondrial ROS production and gene expression alterations related to oxidative stress and ER stress. The adaptive response to increased ROS involves Nrf2-dependent transcription of antioxidant genes which promotes cell survival. Inhibition of the Nrf2-mediated antioxidant mechanism sensitizes breast cancer cells to the lethal effects of Kv11.1 activation.
Correction
Neurosciences
Lucia Privitera, Ellen L. Hogg, Matthias Gaestel, Mark J. Wall, Sonia A. L. Correa
Article
Neurosciences
Li-Ya Jiang, Guan-Hao Wang, Jing-Jiao Xu, Xiao-Li Li, Xiao-Yan Lin, Xiang Fang, Hong-Xu Zhang, Mei Feng, Chun-Ming Jiang
Summary: This study reveals the importance of LINC00473 in regulating temozolomide (TMZ) resistance in glioblastoma (GB) and its potential mechanism. By regulating the expression of CEBP alpha and MGMT, LINC00473 promotes the formation of chemoresistance. Furthermore, LINC00473 can transfer chemoresistance to adjacent sensitive cells through exosomes.
Article
Neurosciences
Olga Kopach, Tetyana Pivneva, Nataliya Fedirko, Nana Voitenko
Summary: This study found that diabetic animals exhibit severe xerostomia characterized by reduced saliva flow rate, diminished total protein content, and decreased amylase activity. The impaired saliva production in diabetes is associated with reduced and delayed intracellular Ca2+ signals in submandibular acinar cells, caused by malfunctioning mitochondria. Targeting malfunctioning mitochondria may be a potential strategy for the treatment of diabetic xerostomia.
Article
Neurosciences
Nicholas M. Timme, Cherish E. Ardinger, Seth D. C. Weir, Rachel Zelaya-Escobar, Rachel Kruger, Christopher C. Lapish
Summary: This study aimed to assess aversion-resistant drinking behavior in head-fixed mice and explore the relationship between non-consummatory behaviors and aversion-resistant drinking. The results showed that head-fixed mice exhibited heterogenous levels of aversion-resistant drinking and non-consummatory behaviors were related to the intensity of this behavior.
Article
Neurosciences
David R. Maguire, Charles P. France
Summary: Methocinnamox (MCAM) is a novel, long-acting opioid receptor antagonist that effectively decreases fentanyl self-administration and prevents opioid overdose in monkeys. The study demonstrates the potential therapeutic utility of MCAM in the treatment of opioid use disorder.
Article
Neurosciences
Xiang Li, Dan Feng, Shenglu Ma, Mingxing Li, Shulei Zhao, Man Tang
Summary: This study investigated the effects of fluoxetine on neurochemical, neurobiological, and neurobehavioral changes in different subregions of the hippocampus. The results showed that fluoxetine increased dialysate 5-HT, decreased membrane 5-HTT protein, and increased cytoplasmic fraction. Additionally, fluoxetine reduced immobility times in behavioral tests, with greater effects observed in the ventral subregion compared to the dorsal subregion.
Article
Neurosciences
Alexander V. Zholos, Mariia I. Melnyk, Dariia O. Dryn
Summary: Acetylcholine is an important neurotransmitter in visceral smooth muscles, activating M2 and M3 muscarinic receptors to cause smooth muscle excitation and contraction. This review focuses on the cellular and molecular mechanisms underlying acetylcholine-induced depolarisation and smooth muscle contraction, as well as the effects of anticholinergic drugs on gastrointestinal motility. The knowledge gained from recent studies has greatly expanded our understanding of these processes.
Article
Neurosciences
Zhenlong Li, Hsien-Yu Peng, Chau-Shoun Lee, Tzer-Bin Lin, Ming-Chun Hsieh, Cheng-Yuan Lai, Han-Fang Wu, Lih-Chyang Chen, Mei-Ci Chen, Dylan Chou
Summary: Methylone shows significant efficacy in treating depression and social deficits, making it an ideal candidate for anti-depressant medication.
Article
Neurosciences
Aline Freyssin, Allison Carles, Sarra Guehairia, Gilles Rubinstenn, Tangui Maurice
Summary: This study explores the potential of combining FENM and S1R agonists in the treatment of Alzheimer's disease. The results showed that most FENM-based combinations can protect against learning deficits caused by A beta 25-35, with better efficacy in short-term memory.
Article
Neurosciences
J. D. Lorente, J. Cuitavi, L. Rullo, S. Candeletti, P. Romualdi, L. Hipolito
Summary: This study analyzed the effects of pain on negative affect in different sexes and time courses, as well as the involvement of the dynorphinergic and corticotropin releasing factor systems in these pain-related behaviors. The results showed sex and time-dependent anxiety- and anhedonia-like behaviors induced by pain in female rats. The recruitment of KOR/DYN in the NAc was identified as a key neurological substrate mediating pain-induced behavioral alterations.
Article
Neurosciences
Rongjun Liu, Daofan Sun, Xiuzhong Xing, Qingge Chen, Bo Lu, Bo Meng, Hui Yuan, Lan Mo, Liufang Sheng, Jinwei Zheng, Qiusheng Wang, Junping Chen, Xiaowei Chen
Summary: The coexistence of pain and depression is frequently observed in patients with chronic pain and depression. Oxytocin, a neuropeptide, has been reported to relieve chronic pain and depressive symptoms. This study investigated the effect of intranasal oxytocin on neuropathic pain and comorbid depressive symptoms, and found that oxytocin attenuated depression-like behavior but did not alleviate mechanical hyperalgesia. The results suggest that intranasal oxytocin may have the potential to treat depressive symptoms in neuropathic pain patients.