Journal
NEUROCHEMICAL RESEARCH
Volume 35, Issue 12, Pages 2135-2143Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s11064-010-0294-0
Keywords
Climbing fiber; Astrocyte; Bergmann glia; CGRP receptor; Neuropeptide; Purkinje cells
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Funding
- Italian Ministry of University and Research
- CNR
- Italian Ministry of Research [2006054051]
- Italian Telethon Foundation [GGP05141]
- Oasi Maria SS, Troina
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Previous reports described the transient expression during development of Calcitonin Gene-Related Peptide (CGRP) in rodent cerebellar climbing fibers and CGRP receptor in astrocytes. Here, mixed cerebellar cultures were used to analyze the effects of CGRP on Purkinje cells growth. Our results show that CGRP stimulated Purkinje cell dendrite growth under cell culture conditions mimicking Purkinje cell development in vivo. The stimulation was not blocked by CGRP8-37, a specific antagonist, suggesting the activation of other related receptors. CGRP did not affect survival of Purkinje cells, granule cells or astrocytes. The selective expression of Receptor Component Protein (RCP) (a component of CGRP receptor family) in astrocytes points to a role of these cells as mediators of CGRP effect. Finally, in pure cerebellar astrocyte cultures CGRP induced a transient morphological differentiation from flat, polygonal to stellate form. It is concluded that CGRP influences Purkinje cell dendrite growth in vitro, most likely through the involvement of astrocytes.
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