Journal
NEURAL REGENERATION RESEARCH
Volume 9, Issue 24, Pages 2142-2150Publisher
SHENYANG EDITORIAL DEPT NEURAL REGENERATION RES
DOI: 10.4103/1673-5374.147948
Keywords
nerve regeneration; Achyranthes bidentata polypeptides; neuroactive component; dorsal root ganglion; neurite outgrowth; crush injury; sciatic nerve; peripheral nerve regeneration; neural regeneration
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Funding
- National Key Basic Research Program of China (973 Program) [2014CB542202]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) in China
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We have previously shown that Achyranthes bidentata polypeptides (ABPP), isolated from Achyranthes bidentata Blume (a medicinal herb), exhibit neurotrophic and neuroprotective effects on the nervous system. To identify the major active component of ABPP, and thus optimize the use of ABPP, we used reverse-phase high performance liquid chromatography to separate ABPP. We obtained 12 fractions, among which the fraction of ABPPk demonstrated the strongest neuroactivity. Immunocytochemistry and western blot analysis showed that ABPPk promoted neurite growth in cultured dorsal root ganglion explant and dorsal root ganglion neurons, which might be associated with activation of Erk1/2. A combination of behavioral tests, electrophysiological assessment, and histomorphometric analysis indicated that ABPPk enhanced nerve regeneration and function restoration in a mouse model of crushed sciatic nerve. All the results suggest that ABPPk, as the key component of ABPP, can be used for peripheral nerve repair to yield better outcomes than ABPP.
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