Journal
NEMATOLOGY
Volume 14, Issue -, Pages 799-804Publisher
BRILL
DOI: 10.1163/156854112X627318
Keywords
adzuki bean; growth pouches; hyper-parasite; infection processes; Meloidogyne incognita
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Funding
- British Council
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The molecular interactions between hosts and parasites is an active area of research, and the parasitism of root-knot nematodes, obligate parasites of plants, by the hyper-parasitic bacterium Pasteuria penetrans offers a model by which to investigate aspects of innate immunity. Using a pouch system we were able to demonstrate by PCR, infection of Meloidogyne incognita 4 days prior to any microscopic observations of parasitism. The pouch system, although not strictly axenic, offered a relatively clean, flexible approach with a greatly reduced number of contaminating microbial species than in any soil-based system, whereby the early stages of nematode infection could be manipulated and controlled.
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