Editorial Material
Multidisciplinary Sciences
Martha W. Bagnall
Summary: Evidence from turtles and computer models suggests that rotational dynamics, a pattern of neuronal activity, determines locomotion, which challenges traditional models of motor control.
Review
Neurosciences
Gonzalo Forno, Albert Llado, Michael Hornberger
Summary: The hippocampus is considered crucial for Alzheimer's disease pathophysiology, but other Papez circuit regions may also be affected, with potential diagnostic value for early AD. Spatial navigation processes within the Papez circuit are gaining recognition for their contribution to AD symptomology.
EUROPEAN JOURNAL OF NEUROSCIENCE
(2021)
Review
Microbiology
Liam J. Worrall, Dorothy D. Majewski, Natalie C. J. Strynadka
Summary: This article describes two fascinating bacterial nanomachines - the widely distributed rotary flagellum and the eukaryotic cell-puncturing injectisome. Both nanomachines utilize the type III secretion system (T3SS) to secrete components and translocate proteins. Advances in structural and functional understanding, facilitated by cryoelectron microscopy technologies, have provided insights into these nanomachines since 2015.
ANNUAL REVIEW OF MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Emily Z. Guo, Jorge E. Galan
Summary: Type III secretion systems are multiprotein molecular machines essential for the virulence of bacterial pathogens. The injectisome mediates the delivery of bacterially encoded proteins into eukaryotic target cells. The structure of the needle tip complex and its interaction with the needle filament are crucial for the translocation of effector proteins.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Biao Yuan, Athina G. Portaliou, Rinky Parakra, Jochem H. Smit, Jiri Wald, Yichen Li, Bindu Srinivasu, Maria S. Loos, Harveer Singh Dhupar, Dirk Fahrenkamp, Charalampos G. Kalodimos, Franck Duong van Hoa, Thorben Cordes, Spyridoula Karamanou, Thomas C. Marlovits, Anastassios Economou
Summary: Type III protein secretion is common in Gram-negative pathogens, with SctV forming peripheral oligomeric clusters in EPEC and serving as a key receptor for different chaperone/exported protein pairs. The dynamic motions of SctV protomers, modulated by chaperones and ATPase, may allosterically affect the secretion process.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Eduardo Soto, Jorge E. Galan, Marfa Lara-Tejero
Summary: In this study, an extensive in vivo cross-linking strategy aided by structure modeling was used to investigate the sorting platform complex of type III secretion systems. The assembly process of this bacterial structure was mapped using identified cross-links as signatures for pairwise intersubunit interactions and systematic genetic deletions. Insights generated by this study could be used for the rational development of antivirulence strategies against medically important bacterial pathogens.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biotechnology & Applied Microbiology
Emel Sen-Kilic, Annalisa B. Huckaby, F. Heath Damron, Mariette Barbier
Summary: This study used RNA-seq analysis to investigate the transcriptome response of type II pneumocytes during P. aeruginosa infection, revealing that P. aeruginosa modulates the proinflammatory response of epithelial cells through T3SS and T6SS, promoting infection progression.
Article
Biochemistry & Molecular Biology
Bailey Milne-Davies, Stephan Wimmi, Andreas Diepold
Summary: The type III secretion system is the common core of two bacterial molecular machines: the flagellum and the injectisome. It is characterized by flexibility, adaptivity, and a dynamic nature.
MOLECULAR MICROBIOLOGY
(2021)
Article
Microbiology
Rory Hennell James, Justin C. Deme, Alicia Hunter, Ben C. Berks, Susan M. Lea
Summary: Gliding motility and protein secretion through the type IX secretion system (T9SS) are two specific features of the Bacteroidetes. These processes are energized by the GldLM motor complex, which converts the proton motive force into mechanical work. The structures of the GldLM motor complex from different Bacteroidetes species reveal a conserved architecture, but with species-specific differences at the N terminus of GldL. This study enhances our understanding of bacterial ion-driven motors and their role in T9SS and gliding motility.
Article
Microbiology
Antoine Zboralski, Adrien Biessy, Martin Filion
Summary: The distribution and functions of Type III secretion systems (T3SSs) in two important bacterial groups, nitrogen-fixing rhizobia and plant-beneficial Pseudomonas spp., are discussed. T3SSs and their effectors play important roles in legume-rhizobia symbiosis and in plant-beneficial Pseudomonas spp. The diversity of T3SSs in plant-beneficial bacteria suggests their significant roles in interkingdom interactions in the rhizosphere.
Article
Fisheries
Zhen Tao, Haoda Ye, Chaozheng Zhang, Suming Zhou, Kequan Wang, Mingming Zhang, Jianjun Xie, Hetron Mweemba Munang'andu, Cheng Xu, Pengcheng Wang, Xiaojun Yan
Summary: In this study, a novel two-component system PvgAS has been identified in P. plecoglossicida, which regulates the expression of T6SS-1 and plays a crucial role in bacterial pathogenicity. This finding provides important insights into the understanding of the pathogenic mechanisms of P. plecoglossicida and potential therapeutic interventions against this bacterium.
Review
Microbiology
Elizabeth A. Rucks
Summary: Type III secretion systems (T3SS) are utilized by Gram-negative pathogens to deliver effector proteins into target eukaryotic cells for manipulating cell functions and enhancing pathogenesis. This review will discuss the history, biochemical characterization, and function of chlamydial T3SS, as well as the use of heterologous/surrogate models for studying it. The review will also cover the history of chlamydial effectors and recent advances in the field.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2023)
Article
Biotechnology & Applied Microbiology
Yushan Xia, Congjuan Xu, Dan Wang, Yuding Weng, Yongxin Jin, Fang Bai, Zhihui Cheng, Oscar P. Kuipers, Weihui Wu
Summary: YbeY is a highly conserved RNase in bacteria that plays essential roles in rRNA processing, small RNA regulation, and bacterial responses to environmental stresses. Mutation of the ybeY gene in Pseudomonas aeruginosa leads to dysregulation of secretion system gene expression and biofilm formation, indicating the pleiotropic functions of YbeY in bacterial regulation.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Dustin E. Bosch, Romina Abbasian, Bishal Parajuli, S. Brook Peterson, Joseph D. Mougous
Summary: Bacteroidales are associated with the severity and progression of inflammatory bowel disease. We have identified the type VI secretion system nuclease effectors (Tde) that are enriched in ulcerative colitis and transferred through mobile genetic elements. Tde-encoding T6SSs mediate interbacterial competition. The orphan and cognate immunity proteins (Tdi) prevent intoxication by multiple Tde through a unique mechanism. This mechanism allows for broad neutralization of Tde by orphan Tdi and may contribute to Bacteroidales diversity in the context of ulcerative colitis.
Article
Plant Sciences
Naama Wagner, Michael Alburquerque, Noa Ecker, Edo Dotan, Ben Zerah, Michelle Mendonca Pena, Neha Potnis, Tal Pupko
Summary: In this study, novel machine-learning algorithms were developed to characterize the secretion signal of Type III effectors in both plant and animal pathogens. A benchmark dataset was curated and the results showed that the novel approach had substantially better classification accuracy compared to previous methodologies. The study also revealed that plant and animal pathogen effectors may have different secretion signals.
FRONTIERS IN PLANT SCIENCE
(2022)
