RNAi screening comes of age: improved techniques and complementary approaches

Online GESS: prediction of miRNA-like off-target effects in large-scale RNAi screen data by seed region analysis

(2014) Bahar Yilmazel et al.   BMC BIOINFORMATICS

To Switch or Not to Switch: At the Origin of Repeat Expansion Disease

(2014) Ekaterina V. Mirkin et al.   MOLECULAR CELL

Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

(2014) Xuebing Wu et al.   NATURE BIOTECHNOLOGY

Functional epigenetics approach identifies BRM/SMARCA2 as a critical synthetic lethal target in BRG1-deficient cancers

(2014) G. R. Hoffman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CRISPR-based technologies: prokaryotic defense weapons repurposed

(2014) Rebecca M. Terns et al.   TRENDS IN GENETICS

In Vivo RNAi Screening Identifies a Leukemia-Specific Dependence on Integrin Beta 3 Signaling

(2013) Peter G. Miller et al.   CANCER CELL

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

A Direct In Vivo RNAi Screen Identifies MKK4 as a Key Regulator of Liver Regeneration

(2013) Torsten Wuestefeld et al.   CELL

Integration of Chemical and RNAi Multiparametric Profiles Identifies Triggers of Intracellular Mycobacterial Killing

(2013) Varadharajan Sundaramurthy et al.   Cell Host & Microbe

Systematic Analysis of RNAi Reports Identifies Dismal Commonality at Gene-Level and Reveals an Unprecedented Enrichment in Pooled shRNA Screens

(2013) Bhavneet Bhinder et al.   COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING

Identification of miRNA modulators to PARP inhibitor response

(2013) Sari Neijenhuis et al.   DNA REPAIR

UP-TORR: Online Tool for Accurate and Up-to-Date Annotation of RNAi Reagents

(2013) Y. Hu et al.   GENETICS

Long Noncoding RNAs: Past, Present, and Future

(2013) J. T. Y. Kung et al.   GENETICS

A genome-wide RNA interference screen identifies new regulators of androgen receptor function in prostate cancer cells

(2013) K. Imberg-Kazdan et al.   GENOME RESEARCH

High-throughput screens identify microRNAs essential for HER2 positive breast cancer cell growth

(2013) Suvi-Katri Leivonen et al.   Molecular Oncology

Genome-wide dissection of the quorum sensing signalling pathway in Trypanosoma brucei

(2013) Binny M. Mony et al.   NATURE

RNAi screens in mice identify physiological regulators of oncogenic growth

(2013) Slobodan Beronja et al.   NATURE

Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library

(2013) Hiroko Koike-Yusa et al.   NATURE BIOTECHNOLOGY

A screen for morphological complexity identifies regulators of switch-like transitions between discrete cell shapes

(2013) Zheng Yin et al.   NATURE CELL BIOLOGY

Mapping genetic interactions in human cancer cells with RNAi and multiparametric phenotyping

(2013) Christina Laufer et al.   NATURE METHODS

A reversible gene trap collection empowers haploid genetics in human cells

(2013) Tilmann Bürckstümmer et al.   NATURE METHODS

A high-throughput screen identifies miRNA inhibitors regulating lung cancer cell survival and response to paclitaxel

(2013) Liqin Du et al.   RNA Biology

The Hippo Signaling Pathway Interactome

(2013) Y. Kwon et al.   SCIENCE

Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

(2013) Ophir Shalem et al.   SCIENCE

Genetic Screens in Human Cells Using the CRISPR-Cas9 System

(2013) Tim Wang et al.   SCIENCE

Protein Complex-Based Analysis Framework for High-Throughput Data Sets

(2013) A. Vinayagam et al.   Science Signaling

Conserved Regulators of Nucleolar Size Revealed by Global Phenotypic Analyses

(2013) R. A. Neumuller et al.   Science Signaling

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

Neuron-Specific Feeding RNAi in C. elegans and Its Use in a Screen for Essential Genes Required for GABA Neuron Function

(2013) Christopher Firnhaber et al.   PLoS Genetics

A CRISPR CASe for high-throughput silencing

(2013) Jacob Heintze et al.   Frontiers in Genetics

Functional Repurposing Revealed by Comparing S. pombe and S. cerevisiae Genetic Interactions

(2012) Adam Frost et al.   CELL

Determination of synthetic lethal interactions in KRAS oncogene-dependent cancer cells reveals novel therapeutic targeting strategies

(2012) Michael Steckel et al.   CELL RESEARCH

Genetic Regulators of a Pluripotent Adult Stem Cell System in Planarians Identified by RNAi and Clonal Analysis

(2012) Daniel E. Wagner et al.   Cell Stem Cell

Genome-Wide RNAi Longevity Screens in Caenorhabditis elegans

(2012) Melana E. Yanos et al.   CURRENT GENOMICS

An RNAi Screen Reveals Intestinal Regulators of Branching Morphogenesis, Differentiation, and Stem Cell Proliferation in Planarians

(2012) David J. Forsthoefel et al.   DEVELOPMENTAL CELL

Single-cell analysis of population context advances RNAi screening at multiple levels

(2012) Berend Snijder et al.   Molecular Systems Biology

On target: A public repository for large-scale RNAi experiments

(2012) Caroline E. Shamu et al.   NATURE CELL BIOLOGY

A bioinformatics method identifies prominent off-targeted transcripts in RNAi screens

(2012) Frederic D Sigoillot et al.   NATURE METHODS

GenomeRNAi: a database for cell-based and in vivo RNAi phenotypes, 2013 update

(2012) Esther E. Schmidt et al.   NUCLEIC ACIDS RESEARCH

Validation of the Cancer BioChip System as a 3D siRNA Screening Tool for Breast Cancer Targets

(2012) Joie N. Marhefka et al.   PLoS One

C911: A Bench-Level Control for Sequence Specific siRNA Off-Target Effects

(2012) Eugen Buehler et al.   PLoS One

Use and Abuse of RNAi to Study Mammalian Gene Function

(2012) W. G. Kaelin   SCIENCE

Cell-based genomic screening: elucidating virus–host interactions

(2012) Debasis Panda et al.   Current Opinion in Virology

Identification of Genes That Promote or Antagonize Somatic Homolog Pairing Using a High-Throughput FISH–Based Screen

(2012) Eric F. Joyce et al.   PLoS Genetics

The maintenance and regeneration of the planarian excretory system are regulated by EGFR signaling

(2011) J. C. Rink et al.   DEVELOPMENT

Shaping Cells and Organs in Drosophila by Opposing Roles of Fat Body-Secreted Collagen IV and Perlecan

(2011) José Carlos Pastor-Pareja et al.   DEVELOPMENTAL CELL

An integrated approach to dissecting oncogene addiction implicates a Myb-coordinated self-renewal program as essential for leukemia maintenance

(2011) J. Zuber et al.   GENES & DEVELOPMENT

Stringent Analysis of Gene Function and Protein-Protein Interactions Using Fluorescently Tagged Genes

(2011) R. A. Neumuller et al.   GENETICS

Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype

(2011) Jennifer L. Rohn et al.   JOURNAL OF CELL BIOLOGY

Functional Identification of Optimized RNAi Triggers Using a Massively Parallel Sensor Assay

(2011) Christof Fellmann et al.   MOLECULAR CELL

RNAi screen identifies Brd4 as a therapeutic target in acute myeloid leukaemia

(2011) Johannes Zuber et al.   NATURE

Ebola virus entry requires the cholesterol transporter Niemann–Pick C1

(2011) Jan E. Carette et al.   NATURE

Mapping of signaling networks through synthetic genetic interaction analysis by RNAi

(2011) Thomas Horn et al.   NATURE METHODS

MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes

(2011) Koen J T Venken et al.   NATURE METHODS

COLT-Cancer: functional genetic screening resource for essential genes in human cancer cell lines

(2011) J. L. Y. Koh et al.   NUCLEIC ACIDS RESEARCH

MicroRNA-520/373 family functions as a tumor suppressor in estrogen receptor negative breast cancer by targeting NF-κB and TGF-β signaling pathways

(2011) I Keklikoglou et al.   ONCOGENE

RNA interference in Caenorhabditis elegans: Uptake, mechanism, and regulation

(2011) JIMMY J. ZHUANG et al.   PARASITOLOGY

Systematic investigation of genetic vulnerabilities across cancer cell lines reveals lineage-specific dependencies in ovarian cancer

(2011) H. W. Cheung et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Essential Gene Profiles in Breast, Pancreatic, and Ovarian Cancer Cells

(2011) Richard Marcotte et al.   Cancer Discovery

RNAi screening: new approaches, understandings, and organisms

(2011) Stephanie E. Mohr et al.   Wiley Interdisciplinary Reviews-RNA

Vigilance and Validation: Keys to Success in RNAi Screening

(2010) Frederic D. Sigoillot et al.   ACS Chemical Biology

Genomic Screening with RNAi: Results and Challenges

(2010) Stephanie Mohr et al.   Annual Review of Biochemistry

A Genome-wide Drosophila Screen for Heat Nociception Identifies α2δ3 as an Evolutionarily Conserved Pain Gene

(2010) G. Gregory Neely et al.   CELL

Systems survey of endocytosis by multiparametric image analysis

(2010) Claudio Collinet et al.   NATURE

Candidate exome capture identifies mutation of SDCCAG8 as the cause of a retinal-renal ciliopathy

(2010) Edgar A Otto et al.   NATURE GENETICS

In Vivo RNAi Rescue in Drosophila melanogaster with Genomic Transgenes from Drosophila pseudoobscura

(2010) Christoph C. H. Langer et al.   PLoS One

Combined agonist-antagonist genome-wide functional screening identifies broadly active antiviral microRNAs

(2010) D. Santhakumar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In vivo RNAi: Today and Tomorrow

(2010) N. Perrimon et al.   Cold Spring Harbor Perspectives in Biology

A Genome-wide RNAi Screen Identifies Multiple Synthetic Lethal Interactions with the Ras Oncogene

(2009) Ji Luo et al.   CELL

What have RNAi screens taught us about viral–host interactions?

(2009) Sara Cherry   CURRENT OPINION IN MICROBIOLOGY

Cross-Species RNAi Rescue Platform in Drosophila melanogaster

(2009) S. Kondo et al.   GENETICS

Discovery of insect and human dengue virus host factors

(2009) October M. Sessions et al.   NATURE

Rapid creation and quantitative monitoring of high coverage shRNA libraries

(2009) Michael C Bassik et al.   NATURE METHODS

Statistical methods for analysis of high-throughput RNA interference screens

(2009) Amanda Birmingham et al.   NATURE METHODS

Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources

(2009) Da Wei Huang et al.   Nature Protocols

Knocking down barriers: advances in siRNA delivery

(2009) Kathryn A. Whitehead et al.   NATURE REVIEWS DRUG DISCOVERY

Haploid Genetic Screens in Human Cells Identify Host Factors Used by Pathogens

(2009) J. E. Carette et al.   SCIENCE

Host Cell Factors in HIV Replication: Meta-Analysis of Genome-Wide Studies

(2009) Frederic D. Bushman et al.   PLoS Pathogens

An Oncogenomics-Based In Vivo RNAi Screen Identifies Tumor Suppressors in Liver Cancer

(2008) Lars Zender et al.   CELL

Genomic RNAi screening in Drosophila S2 cells: what have we learned about host–pathogen interactions?

(2008) Sara Cherry   CURRENT OPINION IN MICROBIOLOGY

The art and design of genetic screens: RNA interference

(2008) Michael Boutros et al.   NATURE REVIEWS GENETICS

Chemical Modification of siRNAs for In Vivo Use

(2008) Mark A. Behlke   OLIGONUCLEOTIDES